Iron Chelation and Septic Arthritis
ironjustice
arthritis caused by Candida albicans.
Lee JH, Park JH, Kim YS, Han Y
Int Immunopharmacol 2008 Aug 26.
There has been an increasing number of studies concerning the multiple
biological activities of polyphenolic compounds.
In this present study, we determined the effect of chlorogenic acid
(CRA), a polyphenolic compound, on septic arthritis caused by Candida
albicans, a major etiological agent that causes fungal arthritis.
To induce septic arthritis, an emulsified mixture of C. albicans cell
wall and Complete Freund's Adjuvant (CACW/CFA) was injected into BALB/
c mice via hind footpad route once a day for 3 days.
Twenty-four hours after the final injection, in order to determine CRA
effect, mice having the swollen footpad were given CRA (1 mg/dose/
mouse) intraperitoneally every other day three times.
The footpad edema was measured for 15 days. Results showed that the
CRA treatment reduced approximately 40% of the edema at the peak of
septic arthritis (P<0.05).
This anti-arthritic activity appeared to be mediated by a complete
inhibition of nitric oxide (NO) production from macrophages and
suppression of T-cells proliferation. Furthermore, CRA also inhibited
growth of C. albicans yeast cells (P<0.01) and caused no hemolysis.
These data indicate that CRA, which has antifungal and anti-arthritic
effects, can safely be administered into the blood circulation for
treatment of septic arthritis due to C. albicans.
In addition, in respect to antiseptic arthritis, it can be suggested
that the anti-candidal effect of CRA may be helpful as an all-in-one
treatment of the candidal arthritis.
International immunopharmacology [Int Immunopharmacol]
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Iron Chelation by Chlorogenic Acid as a Natural Antioxidant
Yasuhisa KONO1), Sakiko KASHINE1), Takushi YONEYAMA1), Yuji
SAKAMOTO1),
Yoshihisa MATSUI1) and Hitoshi SHIBATA1)
1) Department of Life Science and Biotechnology Faculty of Life and
Environmental Science Shimane University
(Received April 28, 1997)
Chlorogenic acid, a dietary antioxidant, effectively inhibited the
iron-induced lipid peroxidation of bovine liver microsomes in a
concentration-dependent manner. In the Fenton-type reaction,
chlorogenic acid inhibited the production of the hydroxyl radical by
iron-EDTA or iron-ADP, while iron plus chlorogenic acid did not
generate the hydroxyl radical. The formation of an iron complex with
chlorogenic acid was demonstrated by UV/vis absorbance spectroscopic,
ESR and 1H-NMR studies. The ferric complex with chlorogenic acid was
in
the ferric high-spin state near rhombicity, and had no radical
scavenging activity. The results indicate that chlorogenic acid
prevented the formation of the hydroxyl radical by forming a chelate
with iron whose complex cannot catalyze the Fenton-type reaction.
Key words: antioxidant; free radical; chelation; chlorogenic acid;
iron
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