New Diagnostic Method Based on the Sound Science of OspA-Induced Diseases
Mort Zuckerman
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Subject: New Diagnostic Method Based on the Sound Science of OspA-
Induced Diseases
Date: Jul 16, 2010 5:38 AM
The seriousness of OspA-Disease or
the diseases-set induced by OspA
blebbing or real vaccination (sometimes
misnamed FibroFemino-itis, but formerly
http://www.actionlyme.org/Pam3Cys_Version15.htm
called the Great and New Great Imitator)
sees first major validation/application:
"Furthermore, it provides some indication of a subacute bacterial
infection, such as borreliosis or tuberculosis. This flow cytometric
method is suitable for clinical diagnostic laboratories, and may help
in the assessment of patients with uncharacteristic inflammatory
symptoms."
http://www.actionlyme.org
http://www.relapsingfever.org
=========================
http://www.ncbi.nlm.nih.gov/pubmed/20626864
MC Infect Dis. 2010 Jul 13;10(1):205. [Epub ahead of print]
Lymphocyte and monocyte flow cytometry immunophenotyping as a
diagnostic tool in uncharacteristic inflammatory disorders.
Janols H, Bredberg A, Thuvesson I, Janciauskiene S, Grip O, Wullt M.
Abstract
ABSTRACT: BACKGROUND: Patients with uncharacteristic inflammatory
symptoms such as long-standing fatigue or pain, or a prolonged fever,
constitute a diagnostic and therapeutic challenge. The aim of the
present study was to determine if an extended immunophenotyping of
lymphocytes and monocytes including activation markers can define
disease-specific patterns, and thus provide valuable diagnostic
information for these patients. METHODS: Whole blood from patients
with gram-negative bacteraemia, neuroborreliosis, tuberculosis, acute
mononucleosis, influenza or a systemic autoimmune disease, as
diagnosed by routine culture and serology techniques was analysed for
lymphocyte and monocyte cell surface markers using a no-wash, no-lyse
protocol for multi-colour flow cytometry method. The immunophenotyping
included the activation markers HLA-DR and CD40. Plasma levels of
soluble TNF alpha receptors were analysed by ELISA. RESULTS: An
informative pattern was obtained by combining two of the analysed
parameters: (i), the fractions of HLA-DR-expressing CD4+ T cells and
CD8+ T cells, respectively, and (ii), the level of CD40 on CD14+ CD16-
monocytes. Patients infected with gram-negative bacteria or EBV showed
a marked increase in monocyte CD40, while this effect was less
pronounced for tuberculosis, borrelia and influenza. The bacterial
agents could be distinguished from the viral agents by the T cell
result; CD4+ T cells reacting in bacterial infection, and the CD8+ T
cells dominating for the viruses. Patients with systemic autoimmunity
also showed a increased activation, but with similar engagement of
CD4+ and CD8+ T cells. Analysis of soluble TNF alpha receptors was
less informative due to a large inter-individual variation.
CONCLUSION: Immunophenotyping including the combination of the
fractions of HLA-DR expressing T cell subpopulations with the level of
CD40 on monocytes produces an informative pattern, differentiating
between bacterial origin, viral origin and systemic autoimmunity.
Furthermore, it provides some indication of a subacute bacterial
infection, such as borreliosis or tuberculosis. This flow cytometric
method is suitable for clinical diagnostic laboratories, and may help
in the assessment of patients with uncharacteristic inflammatory
symptoms.
KMDickson