Selenium stimulates stemness
Kofi
IFATS collection: Selenium induces improvement of stem cell behaviors in
human adipose-tissue stromal cells via SAPK/JNK and stemness acting
signals.
Kim JH, Lee MR, Kim JH, Jee MK, Kang SK.
Department of Physiology, College of Medicine, Pusan National
University, Busan, South Korea.
In the present study, the potential of selenium to enhance stem cell
behavior through improvement of human adipose tissue-derived stromal
cells (ATSCs) and the associated molecular mechanism was evaluated.
Selenium-induced improvement in stem cell behavior of human ATSCs caused
expression of several genes, indicating downregulated mature cell marker
proteins coupled with increased cell growth and telomerase activities
after the overexpression of Rex1, Nanog, OCT4, SOX2, KLF4, and c-Myc.
Also, selenium-treated ATSCs significantly downregulated p53 and p21
tumor suppressor gene products. Selenium induced active growth and
growth enhanced by the activation of signal proteins in ATSCs via the
inhibition of reactive oxygen species-mediated phospho-stress-activated
protein kinase/c-Jun N-terminal protein kinase activation. The
selenium-induced activation of extracellular regulated kinases 1/2 and
Akt in ATSCs resulted in a subsequent induction of the expression of
stemness transcription factors, particularly Rex1, Nanog, and Oct4,
along with definitive demethylation on regulatory regions of Rex-1,
Nanog, and Oct4. The results of our small interfering RNA knockdown
experiment showed that Rex1 plays a major role in the proliferation of
selenium-induced ATSCs. Selenium-treated ATSCs also exhibited more
profound differentiation into mesodermal and neural lineages. We
performed a direct comparison of gene expression profiles in control
ATSCs and selenium-treated ATSCs and delineated specific members of
important growth factor, signaling, cell adhesion, and transcription
factor families. The observations of improved life span and multipotency
of selenium-treated ATSCs clearly indicate that selenium-treated ATSCs
represent an extraordinarily useful candidate cell source for tissue
regeneration. Disclosure of potential conflicts of interest is found at
the end of this article.
Publication Types:
* Research Support, Non-U.S. Gov't
PMID: 18583539
Paul Antonik Wakfer
The free full text is at:
http://www3.interscience.wiley.com/cgi-bin/fulltext/121628374/HTMLSTART
The results of this study suggest to me that selenium should be life
extending. The cell culture selenium concentration (from sodium
selenite) deemed to be optimum was 5ng/ml which would correspond to a
human serum total of only 25mg, a reasonable amount that should be
attainable from supplementation. How about someone here checking out
studies on supplemental dosages versus serum levels for sodium
selenite, so that readers can know how much supplementation is
required to achieve the necessary serum concentration. It would also
be nice to know whether or not other forms of selenium supplements
(particularly sodium selenate) would have the same effects.
--Paul Wakfer
MoreLife for the rational - http://morelife.org
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Paul Antonik Wakfer
Oops! That should have been 25 mcg - with everything else the same.
--Paul Wakfer
MoreLife for the rational -http://morelife.org
Reality based tools for more life in quantity and quality
The Self-Sovereign Individual Project -http://selfsip.org