I want to demonstrate Chop for my class.
I have input the sequence of the human src protein,
which I believe was correctly split into domains in
the past, but I got no division into domains on this run (with
default parameters).
Here is the sequence:
>SRC_HUMAN ID SRC_HUMAN STANDARD; PRT; 535 AA.
GSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEP
KLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDW
WLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESE
TTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLC
HRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLK
PGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYL
RLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTA
RQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERG
YRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
And here is the excerpt from the predictProtein server.
CHOP Proteins into structural domain-like fragments (Liu J.,Rost B)
Result of CHOP prediction (Jinfeng Liu & Burkhard Rost)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Jinfeng Liu & Burkhard Rost
Proteins. (2004) in press
________________________________________________________
Query : predict_h30976
Length: 535
END of results for file predict_h30976
Please advise.
Thanks and best wishes,
Rich
------------------------------------------------------------
Richard A. Friedman, PhD
Associate Research Scientist
Herbert Irving Comprehensive Cancer Center
Oncoinformatics Core
Lecturer
Department of Biomedical Informatics
Box 95, Room 130BB or P&S 1-420C
Columbia University Medical Center
630 W. 168th St.
New York, NY 10032
(212)305-6901 (5-6901) (voice)
frie...@cancercenter.columbia.edu
http://cancercenter.columbia.edu/~friedman/
In memoriam, John Stewart Williamson
The problem has now been fixed. Please resubmit your sequence through
http://rostlab.org/services/chop/submit.html
and let me know if you experience further problems.
Best,
Jinfeng
On Apr 8, 11:32 am, Richard Friedman
> fried...@cancercenter.columbia.eduhttp://cancercenter.columbia.edu/~friedman/
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