[maker-devel] adding a new gene predictor to maker 2

[Dieter Best]

Hello,

is there a way to plugin another gene predictor into the maker2
pipeline or can we only choose between snap, augustus etc

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[Carson Holt]

mRNA-seq data can be passed to MAKER by first processing the data and then putting it into GFF3 format, i.e. process with something like tophat and cufflinks and then convert the output into GFF3.  MAKER comes with tophat2gff3 and cufflinks2gff3 conversion scripts.  Supply the results to the est_gff option in the maker_opt.ctl file.  For ab initio gene predictors, do the same.  Convert the output into GFF3 format then supply the data to pred_gff in the maker_opt.ctl file.  Using GFF3 pass-through you can supply MAKER with evidence from virtually any external source of your choice.  You just have to convert it into GFF3 so MAKER understands how to use it.

Thanks,
Carson