Description:
Flow Cytometry: Critical Assessment of Population Identification Methods
The goal of FlowCAP is to advance the development of computational methods for the identification of cell populations of interest in flow cytometry data. FlowCAP will provide the means to objectively test these methods.
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Preliminary agenda: FlowCAP-2 September 22-23, Bethesda, MD
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Please find attached the preliminary agenda for FlowCAP-2 meeting, next week (September 22-23) on the NIH Campus in Bethesda. We intend to provide participants preliminary results for those challenges we have been able to analyze before the meeting. We will be providing full analysis to all other challenges at a later stage. Please note that evaluation and results are subject to change. To facilitate community interaction and cooperation towards a common goal of understanding, we will only release results to those participants who have provided either pseudocode (i.e., mathematical notation, mixed with the control structures of a conventional programming language, and possibly natural language descriptions), or the actual algorithms involved, or source code, or some combination of all three in sufficient detail so that their results can be reproduced through either re-implementation of their methodology, or (preferably) running the provided source code.... more »
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FlowCAP2 Deadline Extended
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-My apologies if you receive multiple copies of the announcement - Dear participants, The submission deadline (for both results and abstracts) has been extended to 04.Sept 11:59pm (pacific time). Cheers, Nima.
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FlowCAP2 Corrections
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Dear participants, Here are a new set of corrections and clarifications: Challenge 2: Tube 8 is unstained (not an isotype control) and tube 1 is the isotype control. Challenge 3: There are two negative controls per sample. You may choose to use any (or both) of them. Challenge 5: You can ignore CD56-CD23-DUMP-CD11c and FI-1-Fl-2-FL-3-FL-4 folders and... more »
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Challenge 3 raw data
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Dear FlowCAP2 organizers, For challenge 3, would it be possible to provide us with raw (ungated, untransformed) data with a compensation matrix for all tubes from a single patient (i.e. ENV, GAG, negctrl_1, negctrl_2, and sebctrl tubes for patient 049-001)? Cheers, Rob
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FlowCAP-II meeting Sept 22-23 @ NIH Campus
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Free registration<[link]> now open for the FlowCAP-II Summit to be held Sept 22-23 at the NIH campus in Bethesda (Natcher Auditorium). The FlowCAP<[link]> project was established to provide a mechanism to compare and contrast the utility of these novel computational approaches as applied to a common set of reference datasets. The FlowCAP-II Summit will assemble the key stakeholders in field to present and discuss the results from the FlowCAP-II competition, and to discuss how automated methods are being used to address biological questions.... more »
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question about Challenge 7 data
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Hi there, I have a question about the data for challenge 7. In each of the 7 FCS files in this challenge, the total number of markers/channels is 38. Except for FSC and SSC, all other channels appear to be duplicated, according to the name of each channel. I also looked at the data in one of the 7 FCS files. It appears that if two channels share the same name, the raw data for the two channels are highly correlated but not identical.... more »
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FlowCAP2 Correction
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Dear FlowCAP2 participants, For challenge 5, we have decided to limit the analysis to visit 5 - shortly after treatment. Please see attached for more information. Cheers, Nima.
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FlowCAP 2 data
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Greetings, I noticed that the data for the FlowCAP-2 challenges are available as either raw FCS files or pre-transformed and pre-compensated CSV files. I looked in the header of several of the data files for challenge #1 and didn't find a spillover matrix. Will you provide the appropriate compensation matrix(ces) so that we can compensate the FCS files ourselves? For those people using the transformed/compensated CSV files, can you provide details on what transformations you used (and any parameters you set for those transformations)?... more »
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data provider or participant
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HI, I am currently using 12 color flow cytometry to analyze immune cells in the CNS of mice. My analysis is overwhelming and I am looking for solutions. How I can get involved in this project? What data sets would I need to provide etc? Petra Cravens, Ph.D. Dept. of Neurology UTSouthwestern.edu ______________________________ __... more »
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