How can I check a plant is UV resistance or not what are the experiments i can do

[debadutta bhoi]

i want to to know the protocols to check whether a plant is UV resistance. If i want to know the mechanism behind the UV resistance what experiments i should follow. 
Please suggest me some methods and protocols.

Thank you 

Junior Research Fellow 

Sambalpur University, Odisha 
India

[Mega]

Depends on your lab equipment. 

You could irradiate with UV, and then do a RNA -> cDNA to see which genes are turned on then. Maybe some kind of heat shock proteins. 

Or do a HPLC afterwards. If you have access to one. Maybe there are new substances in the leaves then... 

[Jay Woods]

If you want to check if the plant has got a functional UV protection system,

raise it in a growth chamber where you can switch bulbs from visible to UV. In the late 70's we were running tests to determine the dose response curves of apple coloring, post harvest, by light frequency from near IR into UV (280 nm). We learned the hard way to wear UV protecting goggles after we healed up. None of the apples we checked were very resistant to UV.

[Debadutta Bhoi]

Thank You guys for suggestions
I m also trying to design the whole experiment i will expose the plants with different time period than will do comparative study of different pigments and phenoloic compounds as these plays major protective role against the UV

[Debadutta Bhoi]

I need anthrocynin quantification procedure by pH differential method. If any body have please reply or send me links. 

On Fri, Feb 15, 2013 at 10:33 PM, Debadutta Bhoi <debadu...@gmail.com> wrote:

Thank You guys for suggestions
I m also trying to design the whole experiment i will expose the plants with different time period than will do comparative study of different pigments and phenoloic compounds as these plays major protective role against the UV

--
Debadutta Bhoi

Msc 2nd Yr Life Science 

National Institute Of Technology, Rourkela

Odisha

[Debadutta Bhoi]

Thank you Mass Spectrum

On Sat, Feb 23, 2013 at 1:36 AM, Mass Spectrum <massspectru...@gmail.com> wrote:

 

UV is 'converted into' oxidants such as hydrogen peroxide (H(2)O(2)), superoxide anion (O(2)(-)) accumulation, lipid peroxidation (malondialdehyde--MDA), lipoxygenase (LOX) and is handled by antioxidants such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). You can boost those natural plant antixodants by using nitrophenolates:

 

You can read about it here:

Nitrophenolates spray can alter boll abscission rate in cotton through enhanced peroxidase activity and increased ascorbate and phenolics levels

http://www.ncbi.nlm.nih.gov/pubmed/19647335

 

You can get Atonik (cited above) here:

http://massspectrumbotanicals.com/shop/atonik/

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