Question on DNA length detection via electrical methods

[Nathan McCorkle]

Do you think some electrical method could sense single nucleotide DNA
length differences in a femtoliter scale channel? (1micron * 1micron *
2microns = 2 femtoliters)... 1 micron electrodes are easily made with
standard microfab today.

I know we've talked about a few methods on here before, capacitance
comes to mind... or maybe impedance

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[mad_casual]

Depends on what you mean by electrical method (label-free?), but yes.
The problem isn't sensing single nucleotide length differences, the
problem is sensing relevant single nucleotide length differences in
hundreds if not thousands of bases.

[Nathan McCorkle]

Actually I do mean single nucleotide differences in 0-1000 total nucleotides

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[Pat]

If you were able to isolate the strand in question you could probably
run it through something very similar to the nano pore technology in
the new sequencers. Instead of reading the electrical signature and
correlating it with the chemical base you would just read from the
first electrical signal to the last.

http://www.gizmag.com/minion-disposable-dna-sequencer/21513/

On Feb 29, 4:47 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
> Actually I do mean single nucleotide differences in 0-1000 total nucleotides