Can Diybio be my core lab? I am willing to "share"...

[rafael.me...@gmail.com]

When I had funding to do research (not much, and not for long) I was fortunate to have access to a well equipped core lab with all the instruments I needed but couldn't buy. I have the following equipment in my garage-lab that I hope to use and share(meaning you'd be welcome in rural northern Wisconsin if you wanted to run your experiments), 2x 5700 GeneAmp sequence detection systems, an Eppendorf mastercycler PCR machine with a hybridization adaptor, a microtome to cut paraffin embedded tissue blocks, and ribbons of tissue to extract DNA, assorted gel rigs for westerns and for DNA and RNA, a Dynex MRX II microplate reader for Elisas, and Bradford protein quantification, etc. My next project is growing rat glioma tumor cells and doing PCR based single nucleotide mutation using melting curve analysis of my gene of interest, I also want to do chromosome immunoprecipitation to learn the DNA-histone-protein interactions of my gene of interest comparing rat glioma cells versus primary rat astrocytes. For this I'll need to set up a mini-cell culture facility!! I am looking to buy a water jacketed incubator, a Nitrogen cryogenic tank and a cheap biosafety - BSL2- hood /cabinet and an inverted cell culture scope. I'll do anything in my garage that doesn't need IRB approval, no patient samples, that is why I am sticking to rat, human is off limits for the time being. My wife has threatened to divorce me if she catches me getting on ebay to buy stuff, but I just can't help it. I am a brain pathologist and surgical pathologist with a ton of experience with immunohistochemistry, I am happy to help anyone out there with "tissue issues" Cheers.
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