Short bibliography of DIY-friendly DNA synthesis techniques
Bryan Bishop
I put some thought into a small bibliography for DIY DNA synthesis
tonight, after an anonymous fellow and I discussed various methods.
Nothing working yet, and it's not exactly on the top of the list of
things to do in terms of lab components that need to be implemented
(*cough* PCR would be a good first step- Jonathan has some good ideas
on this, though).
Anyway, hope this helps some others thinking about these methods-
http://heybryan.org/books/papers/bibliographies/DIY-DNA-synth.bib
I think I'm missing a lot of different techniques though. For
instance, a while back I found a paper where somebody (foolishly?)
performed benchwork for many, many hours hand-pipetting different
nucleotides around in an attempt to synthesize some 20-mer, which
technically is "DIY friendly", although maybe it's better called
unfriendly- something like that might not be a good idea if you're
looking into synthesizing massive genomes, or something.
