California?

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Tito Jankowski

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Sep 10, 2008, 7:36:48 PM9/10/08
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Are there any DYI-bio members in California? I've relocated to
Sacramento, from Providence, RI and want to stay connected.

Since so many attended biobar camp, who is local (San Fran,
Sacramento)? I would love to show off the "extract DNA from anything
living" protocol.

Tito

On Wed, Sep 10, 2008 at 4:27 PM, Bryan Bishop <kan...@gmail.com> wrote:
>
> On Wednesday 10 September 2008, Mackenzie Cowell wrote:
>> What are some other useful biological parts that the world could do
>> with an open-source version of?
>
> Women? More seriously: ...
>
>> Also, what other projects would everyone like to work on? I've heard
>> some talk of trying to set up some kind of global, distributed
>> biology experiment, something that DIYbio could help invent and test
>> and then distribute - like taking soil samples from all over the
>> world to assay microbial diversity. I think Jason Bobe has more
>> thoughts on this topic.
>
> Another idea is the do-it-yourself bioreactor projects -- to
> package "diybio" into some (somewhat) replicable project for home
> experiments to play with - maybe a first project to start with it would
> be algal insulin production, or the classic rice/tobacco (ok, not
> tobacco) insulin production experiments. Rice is really easy to grow,
> so you could capture some of the home gardening enthusiasts at the same
> time to help out.
>
> http://heybryan.org/~bbishop/docs/ellingtonia/bioreactor/
> http://heybryan.org/~bbishop/docs/ellingtonia/genetic-circuits/
> http://heybryan.org/biotech.git
>
> BTW, there might be some funding from this side of the world for "long
> distance collaboration" - I just learned about it recently, but it
> turns out that there's the possibility of active support. So. :-)
>
> - Bryan
> ________________________________________
> http://heybryan.org/
> Engineers: http://heybryan.org/exp.html
> irc.freenode.net #hplusroadmap
>
> >
>

William Heath

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Sep 10, 2008, 7:47:53 PM9/10/08
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I am in Palo Alto, CA and would love to meet up with you to learn anything you can show me dealing with synthetic biology.

-Tim

Tito Jankowski

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Sep 10, 2008, 8:01:00 PM9/10/08
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Cool man! I've got a good friend down in Woodside who I go visit
pretty frequently. I just finished up at Brown University and really
loved the iGEM team there. Grab me on AIM sometime, titok16.

Also, I think John Cumbers is in your area, too.

Tito

William Heath

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Sep 10, 2008, 8:11:40 PM9/10/08
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I would love it tell me when you coming up next please!!!

slug

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Sep 15, 2008, 12:55:35 PM9/15/08
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Hello
I live in Sacramento and am very interested. Would love to see the
"extract DNA from anything living" protocol and get to know more about
DIYbio.
Scott

William Heath

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Sep 15, 2008, 1:17:05 PM9/15/08
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When are the Californians getting together man?!!!  I am excited to start!

-Tim

nate g

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Sep 15, 2008, 6:21:54 PM9/15/08
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I'm in San Francisco and I'd love to talk science.
I'm interested in bacteriology and oceanography.
The Make zine culture here has been pretty amazing to watch in this
area, and I see a lot of potential for biotech.
Where can I read more about this movement?
-Nate

Bryan Bishop

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Sep 15, 2008, 8:13:41 PM9/15/08
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On Monday 15 September 2008, nate g wrote:
> I'm in San Francisco and I'd love to talk science.

Did you guys miss BioBarCamp or something?

William Heath

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Sep 15, 2008, 8:16:22 PM9/15/08
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I did :(

slug

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Sep 17, 2008, 3:06:44 PM9/17/08
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On Sep 15, 10:17 am, "William Heath" <wghe...@gmail.com> wrote:
> When are the Californians getting together man?!!!  I am excited to start!
> -Tim
>

Please let me know when a gathering might happen. I live in Sac but
also go to the bay area often.
-Scott

William Heath

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Sep 17, 2008, 3:18:51 PM9/17/08
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Hello, can someone take a leadership role in organizing a meeting of Californians?  Its ok, I know we can do it if we try!

-Tim

P.S.

I live in Palo Alto, I will meet anyone anytime, I live for synthetic biology.  Drew Endy is now permanent faculty at Stanford in Palo Alto.  Perhaps someone could arrange for him to speak at a meeting etc...?

Nate Gallagher

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Sep 17, 2008, 3:38:04 PM9/17/08
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There is an event in san francisco called dorkbot that talks about robotics, mechanical engineering, etc. from a DIY artistic perspective
i think they'd be open to anything scientific though ("dork-clone")
there are other make zine events to and some biotech articles were featured in make zine
these seem like good events to start piggybacking DIY bio onto locally, and as attention grows it could spin off into its own semi-monthly event
-Nate

William Heath

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Sep 17, 2008, 3:51:55 PM9/17/08
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Not to send negative, but I really want serious meetings that are literally like linux users group meetings where select people speak on topics and encourage participation/experimentation.  Is there a better venue for that approach?

-Tim

P.S.

I went to the make fair and didn't feel like I learned very much at all :(  I like hands on learning etc...

Nate Gallagher

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Sep 17, 2008, 4:44:06 PM9/17/08
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i agree with you on make faire, but that's also a way to spread the idea and get people interested in getting serious about it.

but also what you're describing is exactly what dorkbot is.
http://dorkbot.org/dorkbotsf/
maybe not so much for public experimentation or direct participation, but there's no problem interrupting to ask questions.
everybody gets a chance to interact with the speaker.

as far as public experimentation, I do think that requires something like a "TechShop" approach as described here by the DIYbio guys:
http://vimeo.com/1087631
you need a safe shop to do this in.
you can supplement your costs by charging for safety classes, project classes, and equipment rentals.
generating the word-of-mouth interest and customer-base for that is what the speaker events are about.

Bryan Bishop

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Sep 17, 2008, 4:57:26 PM9/17/08
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On Wednesday 17 September 2008, William Heath wrote:
> Not to send negative, but I really want serious meetings that are
> literally like linux users group meetings where select people speak
> on topics and encourage participation/experimentation.  Is there a
> better venue for that approach?

I've heard that the perl user groups are legendary for their lightning
talks. You get five minutes and a good sized audience to dump as much
information on them as possible, including a slideshow behind you - if
it fails within the first 1 second you just move on; after you're done
the next guy comes up and dumps information on you too. These guys have
to be very specific and actually giving something of value.

Who's our Larry Wall of biology?

http://bioperl.org/ but it's bioinformatics / programming :)

morethangray

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Sep 17, 2008, 4:57:23 PM9/17/08
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Heya,
I'm happy to take a lead on organizing a meeting for Californians. It
sounds like many of us are in the Bay Area, so I'd throw out SF as a
general meeting location.

Before I pick a place, I need to know how many will attend and what
we're going to do there (i.e. roundtable, presentations, etc). I can
take that info, find a place and send out the invite to a contact
list.

-Morgan

On Sep 17, 12:18 pm, "William Heath" <wghe...@gmail.com> wrote:
> Hello, can someone take a leadership role in organizing a meeting of
> Californians?  Its ok, I know we can do it if we try!
> -Tim
>
> P.S.
>
> I live in Palo Alto, I will meet anyone anytime, I live for synthetic
> biology.  Drew Endy is now permanent faculty at Stanford in Palo Alto.
>  Perhaps someone could arrange for him to speak at a meeting etc...?
>

William Heath

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Sep 17, 2008, 4:59:13 PM9/17/08
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Bryan,

As usual you are way ahead of your time and age.  How do you continue to blow my mind with your maturity and innovation?! :)  As a software developer I want to focus on software problems that solve biology issues.  For instance I went to a transhumanist meeting hoping to meet like minded people but did not succeed.  For instance there are new exciting algorithms that are being developed to isolate sequences of DNA that do certain things that are public domain.  My kingdom to understand and use them and augment them.  My current idea is to create the software to "infer" the dna for a 3d type biological object.  Have you heard of the new opensource LIMS projects?  I was talking to my friend in the bio tech sector and she is using like calculus to correlate dna to protein type measurements etc...  Anyway, I think you get where I am going with it.

-Tim

P.S.

Let the bio games begin!

Tito Jankowski

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Sep 17, 2008, 5:02:35 PM9/17/08
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Count me in! I'm on AIM during the evenings (titok16)
Tito

William Heath

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Sep 17, 2008, 5:03:21 PM9/17/08
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I would like to start an irc channel on the freenode server called diybio.  Anyone into that idea?

-Tim

Tito Jankowski

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Sep 17, 2008, 5:05:21 PM9/17/08
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I <3 freenode
Tito

William Heath

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Sep 17, 2008, 5:10:02 PM9/17/08
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Wow one already exists!  I am in #diybio on freenode now, join me one and all!

-Tim

Tito Jankowski

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Sep 17, 2008, 5:40:53 PM9/17/08
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I'll be there after I get off work....probably around 8 or 9
Tito

Bryan Bishop

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Sep 17, 2008, 8:36:58 PM9/17/08
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On Wednesday 17 September 2008, William Heath wrote:
> My current idea is to create the software to "infer" the dna for a 3d
> type biological object.

It would be useful to have a data set that correlates genome to
resultant 3D objects. That would be a good start.

> Have you heard of the new opensource LIMS projects?

No, but it sounds like EXPO.
http://expo.sf.net/

mlp

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Sep 17, 2008, 11:39:07 PM9/17/08
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On Sep 10, 4:36 pm, "Tito Jankowski" <titojankow...@gmail.com> wrote:
> Are there any DYI-bio members in California? I've relocated to
> Sacramento, from Providence, RI and want to stay connected.
>
> Since so many attended biobar camp, who is local (San Fran,
> Sacramento)? I would love to show off the "extract DNA from anything
> living" protocol.

I just joined the group, and am in San Francisco.

I used to work for Integrated DNA Technologies in their bioinformatics
group, and gave a talk at CodeCon 2005 about SciTools (IDT's web-based
primer design suite). At the beginning of the talk I isolated DNA from
chickpeas using common household items, including a salad spinner for
a centrifuge.

These days I'm a programmer in UC Berkeley's Phylogenomics Research
Group.

Cheers,
--mlp

John Cumbers

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Sep 17, 2008, 11:59:33 PM9/17/08
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Mac/Boston DIYB'ers,
Did you guys solve the centrifuge problem, it just occurred to me that a swing ball might be able to give you those last few extra G's that the salad spinner fails on... it would be fun to try  :)  http://www.toystoreinc.com/catalog/swingball.jpg

I'm also in Bay Area, but quite busy until the new year.  I'd suggest building it and people will come (i.e find a venue, find a speaker and advertise, asking people to sign up to help you gauge numbers along the way).  Maybe Techshop in Menlopark...

John


John Cumbers, Graduate Student
NASA Ames Research Center
Mail Stop 239-20, Bldg N239 Rm 373 Moffett Field, CA 94035, USA.
cell +1 (401) 523 8190, fax +1 (650) 604-1088

Graduate Program in Molecular Biology, Cell Biology, and Biochemistry
Brown University, Box G-W Providence, RI, 02912, USA
swingball.jpg

Bryan Bishop

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Sep 18, 2008, 1:03:56 AM9/18/08
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On Wednesday 17 September 2008, John Cumbers wrote:
> I'm also in Bay Area, but quite busy until the new year.  I'd suggest
> building it and people will come (i.e find a venue, find a speaker
> and advertise, asking people to sign up to help you gauge numbers
> along the way).  Maybe Techshop in Menlopark...

Going with dorkbot is sure to be a good start.

Mackenzie Cowell

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Sep 18, 2008, 2:24:45 PM9/18/08
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Hmm, a swing-ball centrifuge?  I think air resistance becomes one of the main problems preventing high-Gs for simple-machine centrifuges.  Has anyone done the calculations to see how many Gs a salad spinner generates?  I think Jason Morrision and I got up to about 11Gs by swinging a 10 mL falcon tube on the end of a long string over our heads.

I think that one viable strategy for getting lab equipment is to band together with a group of other local diybio enthusiasts, pool some money, (like $500) and talk to the local biotech industry about hand-me-down equipment and places like craigslist.  Many organizations are willing to drop their price by an order of magnitude if you ask and explain it is for an educational, public lab.
My friends here at NUBlabs in boston have been having a lot of success with this.  I'm going to make a post on the diybio blog about the inventory of tools and total pricetag soon.

Cheers,
mac

Tito Jankowski

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Sep 27, 2008, 8:31:35 PM9/27/08
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Hi Morgan,
SF sounds like a great meeting location, I'm more than willing to
drive down (and would welcome any Sacramentians to carpool with me).


Tito

On Wed, Sep 17, 2008 at 1:57 PM, morethangray <hey.mor...@gmail.com> wrote:
>

Meredith L. Patterson

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Sep 27, 2008, 9:01:33 PM9/27/08
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How about an informal get-together to start with, so that we can chat
informally about what sorts of projects we're interested in or working
on and get an idea of whose interests align well? It'd be fun just to
meet everyone in person and boot ideas around.

What's everyone doing next Saturday? From 2pm-11pm at Google San
Francisco, 345 Spear St., there's a hacker party called
SuperHappyDevHouse (http://superhappydevhouse.org/SuperHappyDevHouse27
for more details). It's a big get-together of mostly software geeks,
some hardware geeks, but biogeeks are certainly welcome. I'll be
attending, and we could easily commandeer some tables or couches for a
few hours to throw an informal roundtable.

--mlp

Meredith L. Patterson

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Sep 27, 2008, 9:12:16 PM9/27/08
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Wow, I'm far too fond of the word "informal" today.

Anyway, I threw in a line-item for DIYbio stuff on the
SuperHappyDevHouse wiki -- if anyone wants to drop by and brainstorm,
I'm looking forward to meeting you!

--mlp

William Heath

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Sep 27, 2008, 9:45:18 PM9/27/08
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I am sorry I missed out on this.  I just read this today :(

-Tim

Meredith L. Patterson

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Sep 27, 2008, 10:14:24 PM9/27/08
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It's not today -- it's next Saturday, October 4th. Sorry for the confusion!

--mlp

Tito Jankowski

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Sep 27, 2008, 10:14:37 PM9/27/08
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It didn't happen yet....it's on October 4th. I'm going!
Tito

On Sat, Sep 27, 2008 at 6:45 PM, William Heath <wgh...@gmail.com> wrote:

ArtifexCrastinus

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Sep 28, 2008, 4:18:47 AM9/28/08
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October 4th?! What isn't happening October4th? There's also the San
Francisco MP3 Experiment happening on that same day, in the same city,
starting at the same time! This is ridiculous. Well, at least it will
be over soon (2:45) and I might be able to make it. The distance of SF
and my plans of going to a movie with a friend on her birthday (which
is on that day) will still be a hassle for me though, as I have no
car.

On Sep 27, 7:14 pm, "Tito Jankowski" <titojankow...@gmail.com> wrote:
> It didn't happen yet....it's on October 4th. I'm going!
> Tito
>
> On Sat, Sep 27, 2008 at 6:45 PM, William Heath <wghe...@gmail.com> wrote:
> > I am sorry I missed out on this.  I just read this today :(
> > -Tim
>
> > On Sat, Sep 27, 2008 at 6:12 PM, Meredith L. Patterson <clonea...@gmail.com>

ArtifexCrastinus

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Sep 28, 2008, 5:37:16 AM9/28/08
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Oh, and I live in West Covina, btw. It may be possible for me to get
there by public transit. In fact, I would usggest all that can make
it there do so by pbulic transit as well. I'll have to check the socal
transit website to confirm its feasibility.

Meredith L. Patterson

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Sep 28, 2008, 1:23:13 PM9/28/08
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I strongly suggest public transit as well, not least because it's
nearly impossible to find parking in that part of town -- you'll
either be feeding the meter every hour or paying exorbitant parking
rates. Fortunately, public transit options are many and varied.

Folks coming from Sacramento can take the Capitol Corridor Amtrak,
which takes a bit less than two hours; you'll want to get off at the
Ferry Building, which is about three and a half blocks from 345 Spear
St. (The last train back to Sacramento leaves at 8:25pm that night,
alas.)

I can't offer any educated advice on getting here from Southern
California; Amtrak has the San Joaquins train, which could get you
here and back in a day if sleep doesn't matter to you, or the Coast
Starlight, for which you'll want to leave on Friday and return on
Sunday. (That's hardcore!)

South Bay peeps: your best bet is Caltrain, which will drop you a mile
and a half from the venue. It's a nice walk, or you can pick up the
N-Judah MUNI on the southeast side of 4th and King and take that to
Embarcadero and Harrison. transit.511.org claims this is a 6-minute
ride; this actually varies based on traffic, as it's a surface train.

San Franciscans: the K, T and L MUNI trains all stop at Embarcadero
and Folsom, or take the N-Judah as above. A ridiculous number of buses
have stops within half a mile of the venue, as does the M MUNI at
Embarcadero station. See http://transit.511.org for more details.

East Bayers: BART to Embarcadero and walk half a mile (or take MUNI,
you don't even have to leave the station), or take one of several AC
Transbay buses. Again, see transit.511.org.

--mlp, public transit nerd

ArtifexCrastinus

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Sep 28, 2008, 2:22:09 PM9/28/08
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I think video conferencing would be ideal for me. The distance is
impossible to make on my meager budget, I'm afraid. :( Surely someone
could set up a webcam for those of us that otherwise won't be able to
make it... I know a great website for video conferencing that makes it
all super simple: mebeam.com

On Sep 28, 10:23 am, "Meredith L. Patterson" <clonea...@gmail.com>
wrote:
> I strongly suggest public transit as well, not least because it's
> nearly impossible to find parking in that part of town -- you'll
> either be feeding the meter every hour or paying exorbitant parking
> rates. Fortunately, public transit options are many and varied.
>
> Folks coming from Sacramento can take the Capitol Corridor Amtrak,
> which takes a bit less than two hours; you'll want to get off at the
> Ferry Building, which is about three and a half blocks from 345 Spear
> St. (The last train back to Sacramento leaves at 8:25pm that night,
> alas.)
>
> I can't offer any educated advice on getting here from Southern
> California; Amtrak has the San Joaquins train, which could get you
> here and back in a day if sleep doesn't matter to you, or the Coast
> Starlight, for which you'll want to leave on Friday and return on
> Sunday. (That's hardcore!)
>
> South Bay peeps: your best bet is Caltrain, which will drop you a mile
> and a half from the venue. It's a nice walk, or you can pick up the
> N-Judah MUNI on the southeast side of 4th and King and take that to
> Embarcadero and Harrison. transit.511.org claims this is a 6-minute
> ride; this actually varies based on traffic, as it's a surface train.
>
> San Franciscans: the K, T and L MUNI trains all stop at Embarcadero
> and Folsom, or take the N-Judah as above. A ridiculous number of buses
> have stops within half a mile of the venue, as does the M MUNI at
> Embarcadero station. Seehttp://transit.511.orgfor more details.
>
> East Bayers: BART to Embarcadero and walk half a mile (or take MUNI,
> you don't even have to leave the station), or take one of several AC
> Transbay buses. Again, see transit.511.org.
>
> --mlp, public transit nerd
>

Tito Jankowski

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Oct 4, 2008, 11:47:45 AM10/4/08
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Hi everyone,
DIYbio meetup today at the SuperHappyDev House, downtown SF, 3 pm. See
you there!

Tito

Mackenzie Cowell

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Oct 4, 2008, 12:43:41 PM10/4/08
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Hope it goes well!  Can someone volunteer to give the list an update after the meeting (and also to possibly write a blog post?)

Is anyone going to be in the diybio channel on freenode?  Can anyone plug their webcam into ustream.com or justin.tv to broadcast the event?

Good Luck!
Mac

Meredith L. Patterson

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Oct 4, 2008, 5:06:13 PM10/4/08
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Just an FYI, I may be running a bit late as an interested friend needs
to be picked up. I've borrowed a car and will be bringing equipment to
do serial dilution and plating, and if all goes well, we'll also be
making some yogurt!

See y'all there, sorry for the delay.

--mlp

Meredith L. Patterson

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Oct 4, 2008, 5:09:11 PM10/4/08
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Also -- my cell number is 319-321-1847, feel free to call if anyone
gets lost or anything.

--mlp

On Sat, Oct 4, 2008 at 2:06 PM, Meredith L. Patterson

ArtifexCrastinus

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Oct 4, 2008, 6:08:40 PM10/4/08
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Ok, so where do I go for the video conferencing? Has anybody looked at
mebeam.com?

On Oct 4, 2:09 pm, "Meredith L. Patterson" <clonea...@gmail.com>
wrote:
> Also -- my cell number is 319-321-1847, feel free to call if anyone
> gets lost or anything.
>
> --mlp
>
> On Sat, Oct 4, 2008 at 2:06 PM, Meredith L. Patterson
>
> <clonea...@gmail.com> wrote:
> > Just an FYI, I may be running a bit late as an interested friend needs
> > to be picked up. I've borrowed a car and will be bringing equipment to
> > do serial dilution and plating, and if all goes well, we'll also be
> > making some yogurt!
>
> > See y'all there, sorry for the delay.
>
> > --mlp
>
> >>> Embarcadero station. Seehttp://transit.511.orgfor more details.
>
> >>> East Bayers: BART to Embarcadero and walk half a mile (or take MUNI,
> >>> you don't even have to leave the station), or take one of several AC
> >>> Transbay buses. Again, see transit.511.org.
>
> >>> --mlp, public transit nerd
>

ArtifexCrastinus

unread,
Oct 4, 2008, 6:10:15 PM10/4/08
to DIYbio
If someone could at least be available by IM, that would be great.

On Oct 4, 2:09 pm, "Meredith L. Patterson" <clonea...@gmail.com>
wrote:
> Also -- my cell number is 319-321-1847, feel free to call if anyone
> gets lost or anything.
>
> --mlp
>
> On Sat, Oct 4, 2008 at 2:06 PM, Meredith L. Patterson
>
> <clonea...@gmail.com> wrote:
> > Just an FYI, I may be running a bit late as an interested friend needs
> > to be picked up. I've borrowed a car and will be bringing equipment to
> > do serial dilution and plating, and if all goes well, we'll also be
> > making some yogurt!
>
> > See y'all there, sorry for the delay.
>
> > --mlp
>
> >>> Embarcadero station. Seehttp://transit.511.orgfor more details.
>
> >>> East Bayers: BART to Embarcadero and walk half a mile (or take MUNI,
> >>> you don't even have to leave the station), or take one of several AC
> >>> Transbay buses. Again, see transit.511.org.
>
> >>> --mlp, public transit nerd
>

ArtifexCrastinus

unread,
Oct 4, 2008, 6:11:30 PM10/4/08
to DIYbio
I use Gtalk, MSN, Yahoo, and AIM.
> > >>> Embarcadero station. Seehttp://transit.511.orgformore details.

David Bryson

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Oct 4, 2008, 6:10:57 PM10/4/08
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On Sat, Oct 04, 2008 at 03:08:40PM -0700 or thereabouts, ArtifexCrastinus wrote:
>
> Ok, so where do I go for the video conferencing? Has anybody looked at
> mebeam.com?

I'm not sure that it is 'video conferencing', but here is the live
stream:

http://www.ustream.tv/channel/superhappydevhouse-live-stream

I'm not sure what available resources for actually streaming the meeting
are, but I will look into it.

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