BetaHouse is a co-working space that I work at and that has graciously agreed to host the event. It will work better than Asgard because we're going to be getting our hands dirty:
= AGENDA =
== 7:00 - 8:00 DNA Extraction in the Kitchen == * Read the attached protocol: MAKE vol 07 - Kitchen Counter DNA Lab * The only thing you'll need is to bring your favorite organic material (tuna, banana, cow heart... but keep it safe, please)
== 8:00 - 9:00 - DIYbio Hackathon == * list and tag all DIYbio protocols and resources known to the interwebs * Bring your laptops * add them to del.icio.us: consensus tag "DIYbio" + "protocol", "equipment", "tools" etc. * feel free to get started early * p.s. bryan bishop: we want to produce a high-quality, highly-granular collection of links, not a gigantic disorganized grab-bag
I'll get some pizza for us all (we'll eat while discussing the DNA extraction), so please RSVP so I can get a good headcount.
See you all thursday! Mac
MAKE vol 07 - Kitchen Counter DNA Lab.pdf
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> BetaHouse is a co-working space that I work at and that has graciously > agreed to host the event. It will work better than Asgard because we're > going to be getting our hands dirty:
> = AGENDA =
> == 7:00 - 8:00 DNA Extraction in the Kitchen == > * Read the attached protocol: MAKE vol 07 - Kitchen Counter DNA Lab > * The only thing you'll need is to bring your favorite organic material > (tuna, banana, cow heart... but keep it safe, please)
> == 8:00 - 9:00 - DIYbio Hackathon == > * list and tag all DIYbio protocols and resources known to the interwebs > * Bring your laptops > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", > "equipment", "tools" etc. > * feel free to get started early > * p.s. bryan bishop: we want to produce a high-quality, highly-granular > collection of links, not a gigantic disorganized grab-bag
> I'll get some pizza for us all (we'll eat while discussing the DNA > extraction), so please RSVP so I can get a good headcount.
> == 8:00 - 9:00 - DIYbio Hackathon == > * list and tag all DIYbio protocols and resources known to the > interwebs * Bring your laptops > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", > "equipment", "tools" etc. > * feel free to get started early > * p.s. bryan bishop: we want to produce a high-quality, > highly-granular collection of links, not a gigantic disorganized > grab-bag
Hey now, that's not quite fair. :) If you have any constructive suggestions on the grab bag, be my guest -- I've been lacking creative insights into organizing something like that. A collection of links versus a collection of links plus the actual content has little difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: protocols (that are relevant - maybe 1/12th) to this little 'hackathon' on del.icio.us or whatever it is. Here is my ADR file:
> > == 8:00 - 9:00 - DIYbio Hackathon ==
> > * list and tag all DIYbio protocols and resources known to the
> > interwebs * Bring your laptops
> > * add them to del.icio.us: consensus tag "DIYbio" + "protocol",
> > "equipment", "tools" etc.
> > * feel free to get started early
> > * p.s. bryan bishop: we want to produce a high-quality,
> > highly-granular collection of links, not a gigantic disorganized
> > grab-bag
> Hey now, that's not quite fair. :) If you have any constructive
> suggestions on the grab bag, be my guest -- I've been lacking creative
> insights into organizing something like that. A collection of links
> versus a collection of links plus the actual content has little
> difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re:
> protocols (that are relevant - maybe 1/12th) to this little 'hackathon'
> on del.icio.us or whatever it is. Here is my ADR file:
Thanks to everyone who came out last night to the second meetup - the energy and excitement you all brought was fantastic and I think we are off to a solid start. Besides precipitating DNA from oatmeal, apple, and human saliva, we had a great time tentatively planning out the next couple of months and establishing a draft mission statement and roadmap with milestones:
DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do iGEM-level work in a responsible, safe way. This involves producing educational content, developing successful DIY biotech protocols, developing a larger "process" for conducting diybio work in a safe & open way, and perhaps eventually producing kits and materials.
Over the next month we will put together a comprehensive list of DIY resources that already exist online - protocols, equipment instructions, educational material - and test out some of the most promising experimental techniques, publishing our progress under the auspices of diybio.org. We picked out 3 milestones to work towards:
1. Identify the DIYbio model organism. * It needs to be safe, forgiving, interesting, and available * Candidates include yeast (humanity has a long history of engineering it, and it's commonly available), moss (p. patens?), E. coli (good because it's well understood, bad because it could be pathogenic)
2. Conduct and informally publish a reproducable "$100 transformation", inserting something like GFP into the model organism, or a similar experiment that would be trivial in an academic lab.
3. Form a 2009 DIYbio iGEM team.
I tentatively propose another informal meetup next thursday at 7:00, here at BetaHouse again.
I've never been on a list that had too little traffic - so please don't be afraid to use this one. Bring on the emails. Ask questions. Don't feel stupid. Feel like we are establishing an interesting and educational public record simply by sending emails to one another:)
See you on the list! Mac
On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com <
> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: > > On Monday 09 June 2008, Mackenzie Cowell wrote:
> > > == 8:00 - 9:00 - DIYbio Hackathon == > > > * list and tag all DIYbio protocols and resources known to the > > > interwebs * Bring your laptops > > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", > > > "equipment", "tools" etc. > > > * feel free to get started early > > > * p.s. bryan bishop: we want to produce a high-quality, > > > highly-granular collection of links, not a gigantic disorganized > > > grab-bag
> > Hey now, that's not quite fair. :) If you have any constructive > > suggestions on the grab bag, be my guest -- I've been lacking creative > > insights into organizing something like that. A collection of links > > versus a collection of links plus the actual content has little > > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: > > protocols (that are relevant - maybe 1/12th) to this little 'hackathon' > > on del.icio.us or whatever it is. Here is my ADR file:
On Fri, Jun 13, 2008 at 2:56 PM, Mackenzie Cowell <macow...@gmail.com> wrote: > Howdy Garagistas,
> Thanks to everyone who came out last night to the second meetup - the energy > and excitement you all brought was fantastic and I think we are off to a > solid start. Besides precipitating DNA from oatmeal, apple, and human > saliva, we had a great time tentatively planning out the next couple of > months and establishing a draft mission statement and roadmap with > milestones:
> DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do > iGEM-level work in a responsible, safe way. This involves producing > educational content, developing successful DIY biotech protocols, developing > a larger "process" for conducting diybio work in a safe & open way, and > perhaps eventually producing kits and materials.
> Over the next month we will put together a comprehensive list of DIY > resources that already exist online - protocols, equipment instructions, > educational material - and test out some of the most promising experimental > techniques, publishing our progress under the auspices of diybio.org. We > picked out 3 milestones to work towards:
> 1. Identify the DIYbio model organism. > * It needs to be safe, forgiving, interesting, and available > * Candidates include yeast (humanity has a long history of engineering it, > and it's commonly available), moss (p. patens?), E. coli (good because it's > well understood, bad because it could be pathogenic)
> 2. Conduct and informally publish a reproducable "$100 transformation", > inserting something like GFP into the model organism, or a similar > experiment that would be trivial in an academic lab.
> 3. Form a 2009 DIYbio iGEM team.
> I tentatively propose another informal meetup next thursday at 7:00, here at > BetaHouse again.
> I've never been on a list that had too little traffic - so please don't be > afraid to use this one. Bring on the emails. Ask questions. Don't feel > stupid. Feel like we are establishing an interesting and educational public > record simply by sending emails to one another:)
> See you on the list! > Mac
> On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com > <benjamin.peter...@gmail.com> wrote:
>> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>> > > == 8:00 - 9:00 - DIYbio Hackathon == >> > > * list and tag all DIYbio protocols and resources known to the >> > > interwebs * Bring your laptops >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >> > > "equipment", "tools" etc. >> > > * feel free to get started early >> > > * p.s. bryan bishop: we want to produce a high-quality, >> > > highly-granular collection of links, not a gigantic disorganized >> > > grab-bag
>> > Hey now, that's not quite fair. :) If you have any constructive >> > suggestions on the grab bag, be my guest -- I've been lacking creative >> > insights into organizing something like that. A collection of links >> > versus a collection of links plus the actual content has little >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: >> > protocols (that are relevant - maybe 1/12th) to this little 'hackathon' >> > on del.icio.us or whatever it is. Here is my ADR file:
> I wonder, is there a better way for us to handle picking meeting > times/rsvping? I hate to spam everyone with these "me too" emails...
> Toph
> On Fri, Jun 13, 2008 at 2:56 PM, Mackenzie Cowell <macow...@gmail.com> > wrote: > > Howdy Garagistas,
> > Thanks to everyone who came out last night to the second meetup - the > energy > > and excitement you all brought was fantastic and I think we are off to a > > solid start. Besides precipitating DNA from oatmeal, apple, and human > > saliva, we had a great time tentatively planning out the next couple of > > months and establishing a draft mission statement and roadmap with > > milestones:
> > DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do > > iGEM-level work in a responsible, safe way. This involves producing > > educational content, developing successful DIY biotech protocols, > developing > > a larger "process" for conducting diybio work in a safe & open way, and > > perhaps eventually producing kits and materials.
> > Over the next month we will put together a comprehensive list of DIY > > resources that already exist online - protocols, equipment instructions, > > educational material - and test out some of the most promising > experimental > > techniques, publishing our progress under the auspices of diybio.org. > We > > picked out 3 milestones to work towards:
> > 1. Identify the DIYbio model organism. > > * It needs to be safe, forgiving, interesting, and available > > * Candidates include yeast (humanity has a long history of engineering > it, > > and it's commonly available), moss (p. patens?), E. coli (good because > it's > > well understood, bad because it could be pathogenic)
> > 2. Conduct and informally publish a reproducable "$100 transformation", > > inserting something like GFP into the model organism, or a similar > > experiment that would be trivial in an academic lab.
> > 3. Form a 2009 DIYbio iGEM team.
> > I tentatively propose another informal meetup next thursday at 7:00, here > at > > BetaHouse again.
> > I've never been on a list that had too little traffic - so please don't > be > > afraid to use this one. Bring on the emails. Ask questions. Don't feel > > stupid. Feel like we are establishing an interesting and educational > public > > record simply by sending emails to one another:)
> > See you on the list! > > Mac
> > On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com > > <benjamin.peter...@gmail.com> wrote:
> >> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: > >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
> >> > > == 8:00 - 9:00 - DIYbio Hackathon == > >> > > * list and tag all DIYbio protocols and resources known to the > >> > > interwebs * Bring your laptops > >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", > >> > > "equipment", "tools" etc. > >> > > * feel free to get started early > >> > > * p.s. bryan bishop: we want to produce a high-quality, > >> > > highly-granular collection of links, not a gigantic disorganized > >> > > grab-bag
> >> > Hey now, that's not quite fair. :) If you have any constructive > >> > suggestions on the grab bag, be my guest -- I've been lacking creative > >> > insights into organizing something like that. A collection of links > >> > versus a collection of links plus the actual content has little > >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: > >> > protocols (that are relevant - maybe 1/12th) to this little > 'hackathon' > >> > on del.icio.us or whatever it is. Here is my ADR file:
> On Fri, Jun 13, 2008 at 3:06 PM, Topher Cyll <christopherc...@gmail.com> > wrote:
>> I could make next Thursday.
>> I wonder, is there a better way for us to handle picking meeting >> times/rsvping? I hate to spam everyone with these "me too" emails...
>> Toph
>> On Fri, Jun 13, 2008 at 2:56 PM, Mackenzie Cowell <macow...@gmail.com> >> wrote: >> > Howdy Garagistas,
>> > Thanks to everyone who came out last night to the second meetup - the >> energy >> > and excitement you all brought was fantastic and I think we are off to a >> > solid start. Besides precipitating DNA from oatmeal, apple, and human >> > saliva, we had a great time tentatively planning out the next couple of >> > months and establishing a draft mission statement and roadmap with >> > milestones:
>> > DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do >> > iGEM-level work in a responsible, safe way. This involves producing >> > educational content, developing successful DIY biotech protocols, >> developing >> > a larger "process" for conducting diybio work in a safe & open way, and >> > perhaps eventually producing kits and materials.
>> > Over the next month we will put together a comprehensive list of DIY >> > resources that already exist online - protocols, equipment instructions, >> > educational material - and test out some of the most promising >> experimental >> > techniques, publishing our progress under the auspices of diybio.org. >> We >> > picked out 3 milestones to work towards:
>> > 1. Identify the DIYbio model organism. >> > * It needs to be safe, forgiving, interesting, and available >> > * Candidates include yeast (humanity has a long history of engineering >> it, >> > and it's commonly available), moss (p. patens?), E. coli (good because >> it's >> > well understood, bad because it could be pathogenic)
>> > 2. Conduct and informally publish a reproducable "$100 transformation", >> > inserting something like GFP into the model organism, or a similar >> > experiment that would be trivial in an academic lab.
>> > 3. Form a 2009 DIYbio iGEM team.
>> > I tentatively propose another informal meetup next thursday at 7:00, >> here at >> > BetaHouse again.
>> > I've never been on a list that had too little traffic - so please don't >> be >> > afraid to use this one. Bring on the emails. Ask questions. Don't >> feel >> > stupid. Feel like we are establishing an interesting and educational >> public >> > record simply by sending emails to one another:)
>> > See you on the list! >> > Mac
>> > On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com >> > <benjamin.peter...@gmail.com> wrote:
>> >> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >> >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>> >> > > == 8:00 - 9:00 - DIYbio Hackathon == >> >> > > * list and tag all DIYbio protocols and resources known to the >> >> > > interwebs * Bring your laptops >> >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >> >> > > "equipment", "tools" etc. >> >> > > * feel free to get started early >> >> > > * p.s. bryan bishop: we want to produce a high-quality, >> >> > > highly-granular collection of links, not a gigantic disorganized >> >> > > grab-bag
>> >> > Hey now, that's not quite fair. :) If you have any constructive >> >> > suggestions on the grab bag, be my guest -- I've been lacking >> creative >> >> > insights into organizing something like that. A collection of links >> >> > versus a collection of links plus the actual content has little >> >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks >> re: >> >> > protocols (that are relevant - maybe 1/12th) to this little >> 'hackathon' >> >> > on del.icio.us or whatever it is. Here is my ADR file:
>> On Fri, Jun 13, 2008 at 3:06 PM, Topher Cyll <christopherc...@gmail.com> >> wrote:
>>> I could make next Thursday.
>>> I wonder, is there a better way for us to handle picking meeting >>> times/rsvping? I hate to spam everyone with these "me too" emails...
>>> Toph
>>> On Fri, Jun 13, 2008 at 2:56 PM, Mackenzie Cowell <macow...@gmail.com> >>> wrote: >>> > Howdy Garagistas,
>>> > Thanks to everyone who came out last night to the second meetup - the >>> energy >>> > and excitement you all brought was fantastic and I think we are off to >>> a >>> > solid start. Besides precipitating DNA from oatmeal, apple, and human >>> > saliva, we had a great time tentatively planning out the next couple of >>> > months and establishing a draft mission statement and roadmap with >>> > milestones:
>>> > DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do >>> > iGEM-level work in a responsible, safe way. This involves producing >>> > educational content, developing successful DIY biotech protocols, >>> developing >>> > a larger "process" for conducting diybio work in a safe & open way, and >>> > perhaps eventually producing kits and materials.
>>> > Over the next month we will put together a comprehensive list of DIY >>> > resources that already exist online - protocols, equipment >>> instructions, >>> > educational material - and test out some of the most promising >>> experimental >>> > techniques, publishing our progress under the auspices of diybio.org. >>> We >>> > picked out 3 milestones to work towards:
>>> > 1. Identify the DIYbio model organism. >>> > * It needs to be safe, forgiving, interesting, and available >>> > * Candidates include yeast (humanity has a long history of engineering >>> it, >>> > and it's commonly available), moss (p. patens?), E. coli (good because >>> it's >>> > well understood, bad because it could be pathogenic)
>>> > 2. Conduct and informally publish a reproducable "$100 transformation", >>> > inserting something like GFP into the model organism, or a similar >>> > experiment that would be trivial in an academic lab.
>>> > 3. Form a 2009 DIYbio iGEM team.
>>> > I tentatively propose another informal meetup next thursday at 7:00, >>> here at >>> > BetaHouse again.
>>> > I've never been on a list that had too little traffic - so please don't >>> be >>> > afraid to use this one. Bring on the emails. Ask questions. Don't >>> feel >>> > stupid. Feel like we are establishing an interesting and educational >>> public >>> > record simply by sending emails to one another:)
>>> > See you on the list! >>> > Mac
>>> > On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com >>> > <benjamin.peter...@gmail.com> wrote:
>>> >> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >>> >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>>> >> > > == 8:00 - 9:00 - DIYbio Hackathon == >>> >> > > * list and tag all DIYbio protocols and resources known to the >>> >> > > interwebs * Bring your laptops >>> >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >>> >> > > "equipment", "tools" etc. >>> >> > > * feel free to get started early >>> >> > > * p.s. bryan bishop: we want to produce a high-quality, >>> >> > > highly-granular collection of links, not a gigantic disorganized >>> >> > > grab-bag
>>> >> > Hey now, that's not quite fair. :) If you have any constructive >>> >> > suggestions on the grab bag, be my guest -- I've been lacking >>> creative >>> >> > insights into organizing something like that. A collection of links >>> >> > versus a collection of links plus the actual content has little >>> >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks >>> re: >>> >> > protocols (that are relevant - maybe 1/12th) to this little >>> 'hackathon' >>> >> > on del.icio.us or whatever it is. Here is my ADR file:
Hi All, I am soooo jealous of what your doing!!!! I wish you could like broadcast the meeting on the internet live so I could be there in a cyber way :( Anyone want to do that? Its completely amazing what your doing!
-Tim
P.S.
I am in Palo Alto, CA and would love to startup a DIY bio group out here, let me know if you have ideas or contacts for me to do this.
On Fri, Jun 13, 2008 at 11:56 AM, Mackenzie Cowell <macow...@gmail.com> wrote:
> Thanks to everyone who came out last night to the second meetup - the > energy and excitement you all brought was fantastic and I think we are off > to a solid start. Besides precipitating DNA from oatmeal, apple, and human > saliva, we had a great time tentatively planning out the next couple of > months and establishing a draft mission statement and roadmap with > milestones:
> DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do > iGEM-level work in a responsible, safe way. This involves producing > educational content, developing successful DIY biotech protocols, developing > a larger "process" for conducting diybio work in a safe & open way, and > perhaps eventually producing kits and materials.
> Over the next month we will put together a comprehensive list of DIY > resources that already exist online - protocols, equipment instructions, > educational material - and test out some of the most promising experimental > techniques, publishing our progress under the auspices of diybio.org. We > picked out 3 milestones to work towards:
> 1. Identify the DIYbio model organism. > * It needs to be safe, forgiving, interesting, and available > * Candidates include yeast (humanity has a long history of engineering it, > and it's commonly available), moss (p. patens?), E. coli (good because it's > well understood, bad because it could be pathogenic)
> 2. Conduct and informally publish a reproducable "$100 transformation", > inserting something like GFP into the model organism, or a similar > experiment that would be trivial in an academic lab.
> 3. Form a 2009 DIYbio iGEM team.
> I tentatively propose another informal meetup next thursday at 7:00, here > at BetaHouse again.
> I've never been on a list that had too little traffic - so please don't be > afraid to use this one. Bring on the emails. Ask questions. Don't feel > stupid. Feel like we are establishing an interesting and educational public > record simply by sending emails to one another:)
> See you on the list! > Mac
> On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com < > benjamin.peter...@gmail.com> wrote:
>> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>> > > == 8:00 - 9:00 - DIYbio Hackathon == >> > > * list and tag all DIYbio protocols and resources known to the >> > > interwebs * Bring your laptops >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >> > > "equipment", "tools" etc. >> > > * feel free to get started early >> > > * p.s. bryan bishop: we want to produce a high-quality, >> > > highly-granular collection of links, not a gigantic disorganized >> > > grab-bag
>> > Hey now, that's not quite fair. :) If you have any constructive >> > suggestions on the grab bag, be my guest -- I've been lacking creative >> > insights into organizing something like that. A collection of links >> > versus a collection of links plus the actual content has little >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: >> > protocols (that are relevant - maybe 1/12th) to this little 'hackathon' >> > on del.icio.us or whatever it is. Here is my ADR file:
> DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do > iGEM-level work in a responsible, safe way. This involves producing > educational content, developing successful DIY biotech protocols, > developing a larger "process" for conducting diybio work in a safe & > open way, and perhaps eventually producing kits and materials.
So, one of the ideas I was having for the do-it-yourself biotech kits is the bioreactor that I mentioned on this mailing list a few weeks ago. I don't know why nobody has brought it up, but it's a pretty useful idea for modularizing all of these components and protocols down into something more usable for the amateur and so on. Instead of requiring big, bulky setups for typical benchwork, let's reconceptualize it and integrate the functionality into cells themselves. For example, there's the in vitro DNA synthesizer:
The idea there would be that we could use simple input into a biological system to synthesize DNA in vitro. Hopefully in vivo. That would be even better. But again we come across the purification issues that we were discussing earlier. There's also another problem that I have been running into in the design of the system, mostly the polymerase that would be necessary, but I've been routing around this particular set back by investigating the integration of phosphodiester chemistry into biosynthetic pathways (DTD, for starters, looks proteotoxic, so that's not a good start).
So that larger process, again, can be on the same scale as debian using the same general software infrastructure, such as with content repositories and automated tools, since the majority of biological informatics out there on the internet is already digital it's made all that much more easy.
> Over the next month we will put together a comprehensive list of DIY > resources that already exist online - protocols, equipment > instructions, educational material - and test out some of the most > promising experimental techniques, publishing our progress under the > auspices of diybio.org. We picked out 3 milestones to work towards:
I'll volunteer for those massive reviews. ;-)
> 1. Identify the DIYbio model organism.
There can be more than one.
> * It needs to be safe, forgiving, interesting, and available > * Candidates include yeast (humanity has a long history of > engineering it, and it's commonly available), moss (p. patens?), E. > coli (good because it's well understood, bad because it could be > pathogenic)
All good options.
> 2. Conduct and informally publish a reproducable "$100 > transformation", inserting something like GFP into the model > organism, or a similar experiment that would be trivial in an > academic lab.
So, what I remember from transformation protocols, there's a lot of proprietary chemicals involved, but that should be abstractable away from the system anyway. I would prefer a $0, where all of the materials are biologically produced and purified, or at least a strategy to eventually come to that, and then the $100 version being one where we're "almost there" (and by almost I mean, "well, we have a map").
> I am soooo jealous of what your doing!!!! I wish you could like > broadcast the meeting on the internet live so I could be there in a > cyber way :( Anyone want to do that? Its completely amazing what > your doing!
Since it's Boston I'm sure there's enough local people who know how to set up live streaming video from a web cam. There are also many servers that would be willing to broadcast the stream freely.
Sorry I wasn't able to make yesterday's meeting. As I've been going through the process of setting up an iGem team of my own, I'm intrigued about the idea of a DIYbio iGem team. How would that work? iGem has really been marketed as an undergraduate competition. Of course HS teams have also been welcome. Does that mean only certain people from the DIYbio group would be able to participate?
On Fri, Jun 13, 2008 at 4:27 PM, Bryan Bishop <kanz...@gmail.com> wrote:
> On Friday 13 June 2008, William Heath wrote: > > I am soooo jealous of what your doing!!!! I wish you could like > > broadcast the meeting on the internet live so I could be there in a > > cyber way :( Anyone want to do that? Its completely amazing what > > your doing!
> Since it's Boston I'm sure there's enough local people who know how to > set up live streaming video from a web cam. There are also many servers > that would be willing to broadcast the stream freely.
> Sorry I wasn't able to make yesterday's meeting. As I've been going > through the process of setting up an iGem team of my own, I'm > intrigued about the idea of a DIYbio iGem team. How would that work? > iGem has really been marketed as an undergraduate competition. Of > course HS teams have also been welcome. Does that mean only certain > people from the DIYbio group would be able to participate?
I would think that if you're able to show up in Hong Kong in August, then that you would be allowed to participate. Why not? Let's put everybody's name on the team. That's the idea of collaboration, right? As for actually arranging transportation, that might be a personal means issue, or maybe some of us in educational institutions can go poke some advisors for the money to attend, etc.
> Thanks to everyone who came out last night to the second meetup - the > energy and excitement you all brought was fantastic and I think we are off > to a solid start. Besides precipitating DNA from oatmeal, apple, and human > saliva, we had a great time tentatively planning out the next couple of > months and establishing a draft mission statement and roadmap with > milestones:
I wish i could go to the meetings but i have class on Thursdays until July 4th
> ... > 1. Identify the DIYbio model organism. > * It needs to be safe, forgiving, interesting, and available > * Candidates include yeast (humanity has a long history of engineering it, > and it's commonly available), moss (p. patens?), E. coli (good because it's > well understood, bad because it could be pathogenic)
It may be possible to work with a cryophile, everyone has a fridge and this would take out the pathogenic factor.
> 2. Conduct and informally publish a reproducable "$100 transformation", > inserting something like GFP into the model organism, or a similar > experiment that would be trivial in an academic lab.
BioRad has a protocol and kit for bacterial transformation, this is of course proprietary material but it can be used as a base for the work, it is sold for educational purposes at $88.
Hi Tim, Fantastic! It would be great to start DIYbio interest groups in more cities. All you really need is a few interested folks, a place to meet up, and a loose agenda. The two projects at http://openwetware.org/wiki/DIYbio(kit reviews, model organism research) are both excellent candidates for distributed work and have relatively low barriers to entry, so it may be very fruitful to center discussions on those; perhaps hold mini "kit review jamborees" or "model organism research parties."
On Fri, Jun 13, 2008 at 3:58 PM, William Heath <wghe...@gmail.com> wrote: > Hi All, > I am soooo jealous of what your doing!!!! I wish you could like broadcast > the meeting on the internet live so I could be there in a cyber way :( > Anyone want to do that? Its completely amazing what your doing!
> -Tim
> P.S.
> I am in Palo Alto, CA and would love to startup a DIY bio group out here, > let me know if you have ideas or contacts for me to do this.
> On Fri, Jun 13, 2008 at 11:56 AM, Mackenzie Cowell <macow...@gmail.com> > wrote:
>> Howdy Garagistas,
>> Thanks to everyone who came out last night to the second meetup - the >> energy and excitement you all brought was fantastic and I think we are off >> to a solid start. Besides precipitating DNA from oatmeal, apple, and human >> saliva, we had a great time tentatively planning out the next couple of >> months and establishing a draft mission statement and roadmap with >> milestones:
>> DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do >> iGEM-level work in a responsible, safe way. This involves producing >> educational content, developing successful DIY biotech protocols, developing >> a larger "process" for conducting diybio work in a safe & open way, and >> perhaps eventually producing kits and materials.
>> Over the next month we will put together a comprehensive list of DIY >> resources that already exist online - protocols, equipment instructions, >> educational material - and test out some of the most promising experimental >> techniques, publishing our progress under the auspices of diybio.org. We >> picked out 3 milestones to work towards:
>> 1. Identify the DIYbio model organism. >> * It needs to be safe, forgiving, interesting, and available >> * Candidates include yeast (humanity has a long history of engineering it, >> and it's commonly available), moss (p. patens?), E. coli (good because it's >> well understood, bad because it could be pathogenic)
>> 2. Conduct and informally publish a reproducable "$100 transformation", >> inserting something like GFP into the model organism, or a similar >> experiment that would be trivial in an academic lab.
>> 3. Form a 2009 DIYbio iGEM team.
>> I tentatively propose another informal meetup next thursday at 7:00, here >> at BetaHouse again.
>> I've never been on a list that had too little traffic - so please don't be >> afraid to use this one. Bring on the emails. Ask questions. Don't feel >> stupid. Feel like we are establishing an interesting and educational public >> record simply by sending emails to one another:)
>> See you on the list! >> Mac
>> On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com < >> benjamin.peter...@gmail.com> wrote:
>>> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >>> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>>> > > == 8:00 - 9:00 - DIYbio Hackathon == >>> > > * list and tag all DIYbio protocols and resources known to the >>> > > interwebs * Bring your laptops >>> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >>> > > "equipment", "tools" etc. >>> > > * feel free to get started early >>> > > * p.s. bryan bishop: we want to produce a high-quality, >>> > > highly-granular collection of links, not a gigantic disorganized >>> > > grab-bag
>>> > Hey now, that's not quite fair. :) If you have any constructive >>> > suggestions on the grab bag, be my guest -- I've been lacking creative >>> > insights into organizing something like that. A collection of links >>> > versus a collection of links plus the actual content has little >>> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: >>> > protocols (that are relevant - maybe 1/12th) to this little 'hackathon' >>> > on del.icio.us or whatever it is. Here is my ADR file:
> BioRad has a protocol and kit for bacterial transformation, this is of > course proprietary material but it can be used as a base for the work, it is > sold for educational purposes at $88.
Mario, this sounds great - do you have a link? Might you be able to post it to del.icio.us/tag/diybio or this list?
sorry i couldn't make the meeting, i had a quick details question about your DNA prep. after running through the MAKE protocol, did you by chance run it on a gel to see quality? run it on a spec to see purity?
thanks,
-jamie
On Fri, Jun 13, 2008 at 2:56 PM, Mackenzie Cowell <macow...@gmail.com> wrote:
> Thanks to everyone who came out last night to the second meetup - the > energy and excitement you all brought was fantastic and I think we are off > to a solid start. Besides precipitating DNA from oatmeal, apple, and human > saliva, we had a great time tentatively planning out the next couple of > months and establishing a draft mission statement and roadmap with > milestones:
> DIYbio.org Mission (draft!): To enable enthusiastic amatuers to do > iGEM-level work in a responsible, safe way. This involves producing > educational content, developing successful DIY biotech protocols, developing > a larger "process" for conducting diybio work in a safe & open way, and > perhaps eventually producing kits and materials.
> Over the next month we will put together a comprehensive list of DIY > resources that already exist online - protocols, equipment instructions, > educational material - and test out some of the most promising experimental > techniques, publishing our progress under the auspices of diybio.org. We > picked out 3 milestones to work towards:
> 1. Identify the DIYbio model organism. > * It needs to be safe, forgiving, interesting, and available > * Candidates include yeast (humanity has a long history of engineering it, > and it's commonly available), moss (p. patens?), E. coli (good because it's > well understood, bad because it could be pathogenic)
> 2. Conduct and informally publish a reproducable "$100 transformation", > inserting something like GFP into the model organism, or a similar > experiment that would be trivial in an academic lab.
> 3. Form a 2009 DIYbio iGEM team.
> I tentatively propose another informal meetup next thursday at 7:00, here > at BetaHouse again.
> I've never been on a list that had too little traffic - so please don't be > afraid to use this one. Bring on the emails. Ask questions. Don't feel > stupid. Feel like we are establishing an interesting and educational public > record simply by sending emails to one another:)
> See you on the list! > Mac
> On Thu, Jun 12, 2008 at 11:19 PM, b...@theflowingofthedao.com < > benjamin.peter...@gmail.com> wrote:
>> On Jun 9, 10:43 pm, Bryan Bishop <kanz...@gmail.com> wrote: >> > On Monday 09 June 2008, Mackenzie Cowell wrote:
>> > > == 8:00 - 9:00 - DIYbio Hackathon == >> > > * list and tag all DIYbio protocols and resources known to the >> > > interwebs * Bring your laptops >> > > * add them to del.icio.us: consensus tag "DIYbio" + "protocol", >> > > "equipment", "tools" etc. >> > > * feel free to get started early >> > > * p.s. bryan bishop: we want to produce a high-quality, >> > > highly-granular collection of links, not a gigantic disorganized >> > > grab-bag
>> > Hey now, that's not quite fair. :) If you have any constructive >> > suggestions on the grab bag, be my guest -- I've been lacking creative >> > insights into organizing something like that. A collection of links >> > versus a collection of links plus the actual content has little >> > difference. :-/ I'm wondering where I can dump my 12,000 bookmarks re: >> > protocols (that are relevant - maybe 1/12th) to this little 'hackathon' >> > on del.icio.us or whatever it is. Here is my ADR file:
> BioRad has a protocol and kit for bacterial transformation, this is of >> course proprietary material but it can be used as a base for the work, it is >> sold for educational purposes at $88.
> Mario, this sounds great - do you have a link? Might you be able to post > it to del.icio.us/tag/diybio or this list?
On Tue, Jun 17, 2008 at 11:19 AM, Mario Raya <marior...@gmail.com> wrote: > The kit is part of their Explorers project, intended for high school and > college educators, here is the link (a little long):
> They have plenty of experiments that are intended to high schools, some > require specialized equipment, some not so much, is worthy to look.
> On Sun, Jun 15, 2008 at 3:47 PM, Jason Morrison < > jason.p.morri...@gmail.com> wrote:
>> BioRad has a protocol and kit for bacterial transformation, this is of >>> course proprietary material but it can be used as a base for the work, it is >>> sold for educational purposes at $88.
>> Mario, this sounds great - do you have a link? Might you be able to post >> it to del.icio.us/tag/diybio or this list?
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