Re: DIY Flow Cytometer
Nathan McCorkle
Acoustic Focusing Flow Cytometry a Precise and Sensitive Instrument
for Microparticle
Analysis
C. Blivin1, C. Blivin2, T. A. Woods2, and S. W. Graves2
1University of Rochester, Rochester, NY, 2University of New Mexico,
Albuquerque, NM
Acoustic focusing flow cytometry a precise and sensitive instrument
for microparticle analysis
Christopher Blivin, Travis A. Woods, Steven W. Graves
We are developing a low cost portable flow cytometer for use in
biomedical applications that include blood
counting, HIV progression analysis, and cancer diagnosis. To date we
have built a sheathless low-power
flow cytometer that uses low cost and low power components to perform
microparticle analysis. Our
system uses an acoustic focusing flow-cell, OEM laser pointer modules,
and inexpensive PMT modules.
Here we present experiments to determine the sensitivity, precision,
and maximum analysis rates for our
flow cytometer. We have used calibrated fluorescent microspheres to
determine that our instrument has a
detection limit of less than 200 fluorophores, resolution equivalent
to more expensive benchtop analyzers
that require liquid sheath, precise measurement of alignment
microspheres (<5%CVs), and analysis rates of
greater than 400 particles per second. The current cost of assembly
and performance of this instrument
indicate that refined versions will make powerful flow cytometry
applications available to all clinicians and
researchers worldwide.
On Tue, Sep 28, 2010 at 4:28 PM, ByoWired <byow...@gmail.com> wrote:
> Greetings,
>
> I came across an interesting book entitled, Practical Flow Cytometry,
> by Howard Shapiro. This first edition, which was printed in 1985, has
> instructions on how to make a flow cytometer using commonly available
> items - except for the argon laser, I guess. I've heard that the 2nd
> edition also has these instructions, but the later editions do not.
> Today, the book is up to its 4th edition and I guess it's considered a
> main introduction to flow cytometry. If you Google for it, you can
> probably find a vendor website that will let you download the 4th
> edition for free (also see link below), but to get the building
> instructions you'll need edition 1 or 2, which I think are only
> available at used book stores. Another idea might be to contact the
> author and ask for the instructions, which I hear he is willing to do
> on a case by case basis. He's got a website that has links to some
> other Flow Cytometry downloads, too.
>
> http://www.shapirolab.com/
>
> I'd be interested if anyone has ever built such a device DIY-style or
> has any interest in doing so.
>
> Thanks,
> Mark
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
ByoWired
Cory Tobin
> instructions you'll need edition 1 or 2, which I think are only
> available at used book stores.
Amazon has the older editions too. Here's the 2nd edition, 24 bucks used:
http://www.amazon.com/Practical-flow-cytometry-Howard-Shapiro/dp/0845142542/ref=tmm_hrd_title_2?ie=UTF8&qid=1285706756&sr=8-1
-Cory