ccye...@gmail.com
unread,Sep 13, 2009, 11:55:37 PM9/13/09Sign in to reply to author
Sign in to forward
You do not have permission to delete messages in this group
Either email addresses are anonymous for this group or you need the view member email addresses permission to view the original message
to dChip Software
Dear Dr. Li:
I have downloaded a series of data from GEO sets:
Two sets: U133A, but only text data available
Two sets: U133A, CEL data available
Other sets: U133 plus 2.0, CEL data available
I have combined text format data from the 4 sets using U133A platform
(the so call "series_matrix data"), and used "get external data" read
into dChip and get normalized data. Then used 'export expression value
into text format
For U133 plus 2.0, I used dChip "open group" and read them all and get
normalized data. Then used 'export expression value" to get expression
value of U133A probes set
Then I combined these two sets of data into one, and use "get
external" function again to read into dChip for analysis.
Questions:
(1) Do you think what I did is appropriate?
(2) I used separator to treat U133A group and U133 2.0 group as
different batch to do ANOVA and clustering. Is that necessary? Or not
eough? Or should I just "normalized" all data and treat them the same?
(3) I used gene filter function to select around 12000 genes, and
export these genes into another set of data, and then read them into
dChip using "get external" again. Did I need to normalize these data
again in dChip?