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New to Cytoscape, would like to use proteomic data
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Sarcoma  
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 More options Jun 17, 10:37 am
From: Sarcoma <chairsid...@yahoo.com>
Date: Wed, 17 Jun 2009 07:37:22 -0700 (PDT)
Local: Wed, Jun 17 2009 10:37 am
Subject: New to Cytoscape, would like to use proteomic data
Hello everyone:

I am comparing benign to malignant tumors using mass spec.  I have
data from several patients for each group.  I've looked at the FAQ and
tutorials.  It is unclear to me how best to prepare my data for use in
cytoscape - can I make my own?  what about gene ontology associations?

Sorry if these are incredibly naive questions.

Thanks,

Tim


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piet molenaar  
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 More options Jun 19, 9:52 am
From: piet molenaar <piet....@gmail.com>
Date: Fri, 19 Jun 2009 15:52:42 +0200
Local: Fri, Jun 19 2009 9:52 am
Subject: Re: [cytoscape-helpdesk] New to Cytoscape, would like to use proteomic data

Hi Tim,
The first thing to consider is what you actually want. Map your data onto
networks or derive networks from your data? The first case might be a
starting point. You can import known pathways and annotate these with your
data. In that case I would advise you to study the manual:
http://www.cytoscape.org/manual/Cytoscape2_6Manual.html and more
specifically the attributes section
http://www.cytoscape.org/manual/Cytoscape2_6Manual.html#Node%20and%20...
Deriving networks is no standard procedure; in that case you should consider
specific plugins but I think most of them are geared towards array data...
Hope this helps for a start,
Piet

--
Piet Molenaar
p.molen...@amc.uva.nl
Department of Human Genetics, M1-131
Academic Medical Center
University of Amsterdam
Meibergdreef 9
1105 AZ Amsterdam
the Netherlands

tel (+31) 20-5666592
fax (+31) 20-6918626


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Dariel Ashton-Beaucage  
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 More options Jun 19, 1:16 pm
From: Dariel Ashton-Beaucage <darie...@gmail.com>
Date: Fri, 19 Jun 2009 13:16:17 -0400
Local: Fri, Jun 19 2009 1:16 pm
Subject: Re: [cytoscape-helpdesk] Re: New to Cytoscape, would like to use proteomic data

I'm not a Cytoscape expert, but to map data onto networks, one plugin I
found particularly useful and easy to use is APID2NET (
http://bioinfow.dep.usal.es/apid/apid2net.html). It might be a good one to
try out first. BioNetBuilder (
http://err.bio.nyu.edu/cytoscape/bionetbuilder/index.php) is another nice
one. Maybe this might be useful in your case.
cheers
Dariel
_____________________________
Dariel Ashton-Beaucage
IRIC - Université de Montréal
(514) 343-6111 ext.8092
d.ashton-beauc...@umontreal.ca


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Sarcoma  
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 More options Jun 19, 3:23 pm
From: Sarcoma <chairsid...@yahoo.com>
Date: Fri, 19 Jun 2009 12:23:41 -0700 (PDT)
Local: Fri, Jun 19 2009 3:23 pm
Subject: Re: New to Cytoscape, would like to use proteomic data
Dear Piet:

Thanks for your reply.

> The first thing to consider is what you actually want. Map your data onto
> networks or derive networks from your data?

I would like to map my data.  Now, I have used GoMiner to identify
ontology categories that appear to be active.

Do you have an opinion as to if it would be best (i.e. easiest for a
newbie like me) to map genes or ontology categories?

best regards,

Tim


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Sarcoma  
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 More options Jun 19, 3:25 pm
From: Sarcoma <chairsid...@yahoo.com>
Date: Fri, 19 Jun 2009 12:25:49 -0700 (PDT)
Local: Fri, Jun 19 2009 3:25 pm
Subject: Re: New to Cytoscape, would like to use proteomic data
Dear Dariel:

Very good, I'll go and take a look.

Thank you for these helpful suggestions,

Tim

On Jun 19, 1:16 pm, Dariel Ashton-Beaucage <darie...@gmail.com> wrote:


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