Please comment on this following image about Y Chromosome

[surya prakash]

[laevsky ilana]

Dear Surya Prakash,

To be sure that Y chromosome is normal, you need to do some additional checking: C-banding or FISH for heterochromatin of Y (it's looks like small, but present), FISH to SRY region and (not must) FISH to Y centromere. After all of these the Y's structure will be  more clear to you.

Regards,

 

Ilana

From: surya prakash <suryaprak...@gmail.com>
To: cytogenetics-methods...@googlegroups.com
Sent: Monday, May 13, 2013 8:12 AM
Subject: Please comment on this following image about Y Chromosome

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[maria mouriki]

Could you please provide  me with the following info?

1. what sample is that?

2. what is the referral reason?

3. can you attach a photo with longer chromosomes? (even just the Y chromosome)

4. Have you performed C-banding at all?

5. Have you done any FISH for Yp probes?

6. Does Y look like it in all cells?

 

Maria

[Elisabet Lloveras Caballe]

Hi Surya,

I think that you must to do a FISH using SRY probe but in metaphases in order to visualize if it is an abnormal chromosome Y.

eli

[Maria Kontodiou]

Dear Surya Prakash,

You should do FISH for SRY. It is normal to find different sizes (polymorphism) of the Y chromosome!

Sent from my iPhone

On 13 Μαϊ 2013, at 8:12, surya prakash <suryaprak...@gmail.com> wrote:

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<Y chromosome.TIF>

[maria mouriki]

Many mentioned FISH for SRY. If you are gonna do FISH you may wanna do FISH for the SHOX gene too. In addition if you want to take it a step further you may want to do array-CGH.

 

Maria

From: surya prakash <suryaprak...@gmail.com>
To: cytogenetics-methods...@googlegroups.com
Sent: Monday, May 13, 2013 8:12 AM

Subject: Please comment on this following image about Y Chromosome

[suguru revathi]

hi surya this is revathi can u send one/two more images...to conform...

[mukkanti kasaragadda]

Mr.Prakash,kalavakuru

can you tell me what exactly your opinion about this picture.

On Monday, May 13, 2013 10:42:16 AM UTC+5:30, surya prakash wrote:

[Ashish Fauzdar]

Dear Surya,

I would suggest to get some more karyos with better resolution and try to get good length of chromsomes with colichine and time first before you can plan for FISH probes for SRY,SHOX gene (if available).

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Thanks & Regards

Ashish Fauzdar, PhD

[ritu rao]

Hi Surya,

First of all You have to compare the lenght of Y chromosome in all the cells . If it looks similar in all cells , then there is no point to worry. But if the length of Y is different in different cells, then you might as well compare the clinical interpretation and indication. If you planning to do a FISH, you first have to use a probe to make sure the presence of Y , because sometimes it can be a marker chromosome as well . I think you have SRY probes readily available so you can confirm it with that. Before reporting it is important to relate the clinical interpretation .