Bone Marrow culture

[Saeedeh Ghazaey]

Dear colleagues

I have a big problem with Bone Marrow culture, because  when I spread the suspension, I have a lot of cells without any metaphase, so I can not karyotype the slides.

Do you have any solution for me?

Thanks

Ghazaey

[Marjori Leiva Camparoto]

Hi Ghazaey!

Here in Barretos Cancer Hospitla make the quantification of leukocytes
before placing them in culture


Marjori

2012/6/9, Saeedeh Ghazaey <ghaz...@gmail.com>:

> --
> You received this message because you are subscribed to the Google Groups
> "Cytogenetics methods and trouble-shooting Forum" group.
> To post to this group, send an email to
> cytogenetics-methods...@googlegroups.com.
> To unsubscribe from this group, send email to
> cytogenetics-methods-and-t...@googlegroups.com.
> For more options, visit this group at
> http://groups.google.com/group/cytogenetics-methods-and-trouble-shooting?hl=en-GB.
>
>


--
*Marjori Leiva Camparoto, PhD*
*Human Biology Researcher*
*Barretos Cancer Hospital*
*Rua Antenor Duarte Villela, 1331*
*CEP 14784400, Barretos, São Paulo, Brazil*
*Phone 173321-6600 Extension: 6963*

[Saeedeh Ghazaey]

Dear Marjori

Thanks for your consideration.
First of all we count the number of leukocytes. We have not any problem in culture but we could not find any metaohases in the slides.

Ghazaey

[Vaibhav Pancholi]

dear marjori 

i have also this type of problem so pls suggest some solutions

On Monday, June 11, 2012 5:31:36 PM UTC+5:30, Saeedeh Ghazaey wrote:

Dear Marjori

Thanks for your consideration.
First of all we count the number of leukocytes. We have not any problem in culture but we could not find any metaohases in the slides.

Ghazaey

On Sun, Jun 10, 2012 at 10:52 PM, Marjori Leiva Camparoto <marj...@gmail.com> wrote:

Hi Ghazaey!

Here in Barretos Cancer Hospitla make the quantification of leukocytes
before placing them in culture


Marjori

2012/6/9, Saeedeh Ghazaey <ghaz...@gmail.com>:

> Dear colleagues
>
> I have a big problem with Bone Marrow culture, because  when I spread the
> suspension, I have a lot of cells without any metaphase, so I can not
> karyotype the slides.
>
> Do you have any solution for me?
>
> Thanks
>
> Ghazaey
>

> --
> You received this message because you are subscribed to the Google Groups
> "Cytogenetics methods and trouble-shooting Forum" group.
> To post to this group, send an email to

> cytogenetics-methods-and-trouble-shooting@googlegroups.com.

> To unsubscribe from this group, send email to

> cytogenetics-methods-and-trouble-shooting+unsubscribe@googlegroups.com.

> For more options, visit this group at
> http://groups.google.com/group/cytogenetics-methods-and-trouble-shooting?hl=en-GB.
>
>

--
*Marjori Leiva Camparoto, PhD*
*Human Biology Researcher*
*Barretos Cancer Hospital*
*Rua Antenor Duarte Villela, 1331*
*CEP 14784400, Barretos, São Paulo, Brazil*
*Phone 173321-6600 Extension: 6963*

--
You received this message because you are subscribed to the Google Groups "Cytogenetics methods and trouble-shooting Forum" group.

To post to this group, send an email to cytogenetics-methods-and-trouble-shooting@googlegroups.com.
To unsubscribe from this group, send email to cytogenetics-methods-and-trouble-shooting+unsubscribe@googlegroups.com.

[ibnfal...@gmail.com]

Hi,

 

what is the cell concentration that you use per culture? how long do you incubate the culture? describe your harevesting steps?

 

the conditions of dropping your slides temperature and humidity?