cytoclear
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Jing
Feb 3, 2012, 1:20:30 PM2/3/12
to Cytogenetics methods and trouble-shooting Forum
Hollow, everyone,
I am a UT M.D. Anderson Cancer School of Health Profession student. I
am doing the experimental about cytoclear. Who has the information
about it, such as reference, protocol, and any thing you know. Please
e-mail me at
jingb...@yahoo.com
Thanks a lot.
Jing
I am a UT M.D. Anderson Cancer School of Health Profession student. I
am doing the experimental about cytoclear. Who has the information
about it, such as reference, protocol, and any thing you know. Please
e-mail me at
jingb...@yahoo.com
Thanks a lot.
Jing
stuarthold
Feb 7, 2012, 6:29:50 AM2/7/12
to Cytogenetics methods and trouble-shooting Forum
Hi Jing
try this article
LINE-1 Elements: Analysis by Fluorescence In-Situ Hybridization and
Nucleotide Sequences
by Paul D. Waters, Gauthier Dobigny, Peter J. Waddell and Terence J.
Robinson
Phylogenomics
Methods in Molecular Biology, 2008, Volume 422, 227-237, DOI:
10.1007/978-1-59745-581-7_14
On Feb 4, 5:20 am, Jing <jingbao2...@yahoo.com> wrote:
> Hollow, everyone,
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
> jingbao2...@yahoo.com
>
> Thanks a lot.
>
> Jing
try this article
LINE-1 Elements: Analysis by Fluorescence In-Situ Hybridization and
Nucleotide Sequences
by Paul D. Waters, Gauthier Dobigny, Peter J. Waddell and Terence J.
Robinson
Phylogenomics
Methods in Molecular Biology, 2008, Volume 422, 227-237, DOI:
10.1007/978-1-59745-581-7_14
On Feb 4, 5:20 am, Jing <jingbao2...@yahoo.com> wrote:
> Hollow, everyone,
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
>
> Thanks a lot.
>
> Jing
marg
Feb 9, 2012, 10:42:41 PM2/9/12
to Cytogenetics methods and trouble-shooting Forum
Jing try the vendor's webpage its the best way to start your search
and it appears may companies distribute cytoclear.
http://www.genialgenetics.com/products/reagents/cytoclear-cytoplasm-remover.html
and it appears may companies distribute cytoclear.
http://www.genialgenetics.com/products/reagents/cytoclear-cytoplasm-remover.html
> Hollow, everyone,
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
andrew
Feb 10, 2012, 8:47:55 AM2/10/12
to Cytogenetics methods and trouble-shooting Forum
Hi
As far as my knowledge goes its the only way to
save a poor harvest caused by improver hypotonic treatment. It works
fine with our archival cell suspensions and F.I.S.H slides to ensure
better probe signals.
As far as my knowledge goes its the only way to
save a poor harvest caused by improver hypotonic treatment. It works
fine with our archival cell suspensions and F.I.S.H slides to ensure
better probe signals.
On Feb 4, 5:20 am, Jing <jingbao2...@yahoo.com> wrote:
> Hollow, everyone,
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
kanditry
Feb 15, 2012, 6:49:48 AM2/15/12
to Cytogenetics methods and trouble-shooting Forum
Cytoclear is cleans the slides used for fluorescent in situ
hybridization.
Here is a useful reference:
In Vitro Cell Dev Biol Anim. 2011 Feb;47(2):139-48. Epub 2010 Nov 17.
Selection for EGFR gene amplification in a breast epithelial cell line
with basal-like phenotype and hereditary background.
Ingthorsson S, Halldorsson T, Sigurdsson V, Friðriksdottir AJ,
Bodvarsdottir SK, Steinarsdottir M, Johannsson O, Magnusson MK,
Ogmundsdottir HM, Gudjonsson T.
SourceStem Cell Research Unit, Biomedical Center, University of
Iceland and Department of Laboratory Hematology, Landspitali
University Hospital, Reykjavik, Iceland.
Abstract
An epithelial cell line, referred to as A163, was established from
breast carcinoma derived from a patient with a strong family history
of breast cancer but no known breast cancer susceptibility mutation.
A163 was propagated in a serum-free culture medium including the
epidermal growth factor. Immunophenotypic characterization
demonstrated a mixed luminal and basal-like phenotype. When epidermal
growth factor was excluded from the culture medium, A163 entered a
quiescent period followed by a period of increased cell proliferation
in a subpopulation of the cells. The epidermal growth factor-
independent subpopulation retained the basal-like phenotype of the
parental cell line. Karyotype and fluorescent in situ hybridization
analysis showed an amplification of epidermal growth factor receptor
on 7q in A163-S1 only, resulting in high expression of total and
phosphorylated epidermal growth factor receptor. The A163-S1 sub-line
piles up in culture, indicating a loss of contact inhibition. When
grown on transwell filters, A163 shows basal expression of P63 and
cytokeratin 14, whereas A163-S1 expresses P63 ubiquitously, and has
lost the basal specific expression of cytokeratin 14, indicating a
loss of polarity. Furthermore, when cultured in reconstituted basement
membrane matrix, A163 form polarized normal like acini. In contrast,
A163-S1 form large disorganized structures with lack of polarity.
These cell lines may prove useful to understand molecular changes in
breast cancer progression, in particular basal-like breast cancer
subtype with bad prognosis and no current treatment options.
hybridization.
Here is a useful reference:
In Vitro Cell Dev Biol Anim. 2011 Feb;47(2):139-48. Epub 2010 Nov 17.
Selection for EGFR gene amplification in a breast epithelial cell line
with basal-like phenotype and hereditary background.
Ingthorsson S, Halldorsson T, Sigurdsson V, Friðriksdottir AJ,
Bodvarsdottir SK, Steinarsdottir M, Johannsson O, Magnusson MK,
Ogmundsdottir HM, Gudjonsson T.
SourceStem Cell Research Unit, Biomedical Center, University of
Iceland and Department of Laboratory Hematology, Landspitali
University Hospital, Reykjavik, Iceland.
Abstract
An epithelial cell line, referred to as A163, was established from
breast carcinoma derived from a patient with a strong family history
of breast cancer but no known breast cancer susceptibility mutation.
A163 was propagated in a serum-free culture medium including the
epidermal growth factor. Immunophenotypic characterization
demonstrated a mixed luminal and basal-like phenotype. When epidermal
growth factor was excluded from the culture medium, A163 entered a
quiescent period followed by a period of increased cell proliferation
in a subpopulation of the cells. The epidermal growth factor-
independent subpopulation retained the basal-like phenotype of the
parental cell line. Karyotype and fluorescent in situ hybridization
analysis showed an amplification of epidermal growth factor receptor
on 7q in A163-S1 only, resulting in high expression of total and
phosphorylated epidermal growth factor receptor. The A163-S1 sub-line
piles up in culture, indicating a loss of contact inhibition. When
grown on transwell filters, A163 shows basal expression of P63 and
cytokeratin 14, whereas A163-S1 expresses P63 ubiquitously, and has
lost the basal specific expression of cytokeratin 14, indicating a
loss of polarity. Furthermore, when cultured in reconstituted basement
membrane matrix, A163 form polarized normal like acini. In contrast,
A163-S1 form large disorganized structures with lack of polarity.
These cell lines may prove useful to understand molecular changes in
breast cancer progression, in particular basal-like breast cancer
subtype with bad prognosis and no current treatment options.
> Hollow, everyone,
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
>
> I am a UT M.D. Anderson Cancer School of Health Profession student. I
> am doing the experimental about cytoclear. Who has the information
> about it, such as reference, protocol, and any thing you know. Please
> e-mail me at
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