Missing spectra numbers when searching MGF based spectrarecords with Tide

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Johannes

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Feb 2, 2012, 5:09:40 AM2/2/12
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Hi group,

I've converted several (well formatted) MGF files to spectrarecords using the latest (modified) version of msconvert that's shipped with Tide (1.0-x86_64-binaries). In the Tide output (sqt and text) all spectra numbers are reported as "0". Here's an excerpt of the Tide text output:

0       1970.90 3
        0.209824        LFSLVIWICLVAMGYLFMCFGGTVSPWDQVSFFLFIIFVVYTMLPFNMR
                sp|Q08462|ADCY2_HUMAN   107     R       D
0       1971.60 3
        0.287774        LNVWVIVMIDLCTHTLGTHNTMLSVESDQFALFSLSEQTLAVHCHLCMYC
                tr|DECOY_E7ERY2|E7ERY2_HUMAN    561     R       -

Personally, I would expect to find the spectrum's index within the MGF file as it's normally present when using Crux on MS2 files (when I converted the MGF files to MS2 and then used Crux to search them the output was as expected).

Thanks for any help,
Johannes
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