Johannes
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Hi group,
I've converted several (well formatted) MGF files to spectrarecords using the latest (modified) version of msconvert that's shipped with Tide (1.0-x86_64-binaries). In the Tide output (sqt and text) all spectra numbers are reported as "0". Here's an excerpt of the Tide text output:
0 1970.90 3
0.209824 LFSLVIWICLVAMGYLFMCFGGTVSPWDQVSFFLFIIFVVYTMLPFNMR
sp|Q08462|ADCY2_HUMAN 107 R D
0 1971.60 3
0.287774 LNVWVIVMIDLCTHTLGTHNTMLSVESDQFALFSLSEQTLAVHCHLCMYC
tr|DECOY_E7ERY2|E7ERY2_HUMAN 561 R -
Personally, I would expect to find the spectrum's index within the MGF file as it's normally present when using Crux on MS2 files (when I converted the MGF files to MS2 and then used Crux to search them the output was as expected).
Thanks for any help,
Johannes