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Message from discussion Auction P&G Cincinnati: Science equipmet, camera, video, automation/robot equipment

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Date: Mon, 30 Jul 2012 18:47:35 -0400
Message-ID: <CALEb9cWNGcsF7QcBN6ibHn6xpYLL8rxmU_Nwjs0XYu6buYE...@mail.gmail.com>
Subject: Re: [CHP] Re: Auction P&G Cincinnati: Science equipmet, camera,
 video, automation/robot equipment
From: Craig <agent.cr...@gmail.com>
To: cincihackerspace@googlegroups.com
Cc: horwa...@mail.uc.edu
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Enable 3rd world countries or build a Hive1tree.  Damn it! Why must I
choose! Lol
On Jul 30, 2012 5:20 PM, "Addison" <nos...@gmail.com> wrote:

> Mike,
> It's an idea I've been kicking around for a couple of years.  It's to coo=
k
> up a plasmid based generalizable self stablizing protein expression syste=
m
> running on yogurt bacteria coculture.  The idea is to exploit the native
> antibiotic peptides production that some strains of Lactobacillus produce
> as a selection factor maintaining the protein expression plasmid in a
> second strain.  At the end ideally the system would end up as something
> that could be deployed easily to 3rd world people with a yogurt culturing
> knowledge base and some polypeptide medical need.
>
> A first protein expression target could be Human intrinsic factor (HIF).
> The gut stops/slows producing it in many people around age 60 leading to =
b
> vitamin deficiencies that cause dementia/alzheimers like symptoms. In acu=
te
> cases if it's detected (a big if)the approach to treating it is to use
> animal sourced intrinsic factor.  There is of course a pile of
> regulation/industrial capital required to allow that production.  Stickin=
g
> it in yogurt would be comparatively simple (if it works) and allow
> production anywhere there is milk and a warm clean pot.
>
> Alternately we could muck around with some apple strain selection and mak=
e
> the hive1tree.
>
> -AC
>
>
> On Thursday, July 26, 2012 5:00:56 PM UTC-4, Michael Horwath wrote:
>>
>> Not yet myself, unless you count yeast culture (beer brewing :D...) but
>> I'd definitely be up for getting involved with an OSbio project!  I'm an
>> immunology graduate student so I do various bio techniques as my "day
>> job."  What kind of protein synthesis project do you have in mind?
>>
>> Mike
>>
>> On Thu, Jul 26, 2012 at 4:40 PM, Addison
>>
>>> Yeah, I'm going to pick up some of the equipment. It'll be fun to do
>>> some of the DIY bio stuff, I've got some neat thoughts on protein synth=
esis
>>> that should be fairly straight forward to implement with a minimal amou=
nt
>>> of technology.  Is anyone doing OSbio at the hackspace these days?
>>>
>>> On Tuesday, July 24, 2012 5:01:59 PM UTC-4, Addison wrote:
>>>>
>>>> Hey Y'all,
>>>>
>>>> It's been a while, but this seems to be Hive13 cromulent.  P&G mason,
>>>> cinci, st bernard, and west chester are getting rid of lots of
>>>> industrial/research stuff.  The auction starts tomorrow (7/25) and end=
s on
>>>> thursday (7/26)
>>>>
>>>> It Includes:
>>>> cameras (old digital/old analogue)
>>>> video cameras
>>>> balances
>>>> constant temperature baths
>>>> densitometers
>>>> incubators
>>>> switches/hubs
>>>> LN2 tanks
>>>> etc.
>>>>
>>>> Here's a link: http://www.go-dove.com/en/**even**
>>>> t-16591/Procter-Gamble-**Sale-**21-(North-America-USA-**Canada)/**
>>>> lots?Action=3DMove&AP=3D1&**Auction_**ID=3D0&PP=3D50&Show=3Dall&**Even=
tID=3D**
>>>> 16591&LF=3DCincinnati%**2C+OH+&**SortBy=3D**paddedinventoryid&**SortDi=
r=3Dasc<http://www.go-dove.com/en/event-16591/Procter-Gamble-Sale-21-%28Nor=
th-America-USA-Canada%29/lots?Action=3DMove&AP=3D1&Auction_ID=3D0&PP=3D50&S=
how=3Dall&EventID=3D16591&LF=3DCincinnati%2C+OH+&SortBy=3Dpaddedinventoryid=
&SortDir=3Dasc>
>>>>
>>>> Good luck!
>>>>
>>>>  --
>>> You received this message because you are subscribed to the Google
>>> Groups "Hive13 Hackerspace" group.
>>>
>>
>>  --
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<p dir=3D"ltr">Enable 3rd world countries or build a Hive1tree.=A0 Damn it!=
 Why must I choose! Lol</p>
<div class=3D"gmail_quote">On Jul 30, 2012 5:20 PM, &quot;Addison&quot; &lt=
;<a href=3D"mailto:nos...@gmail.com">nos...@gmail.com</a>&gt; wrote:<br typ=
e=3D"attribution"><blockquote class=3D"gmail_quote" style=3D"margin:0 0 0 .=
8ex;border-left:1px #ccc solid;padding-left:1ex">
Mike,<br>It&#39;s an idea I&#39;ve been kicking around for a couple of year=
s.=A0 It&#39;s to cook up a plasmid based generalizable self stablizing pro=
tein expression system running on yogurt bacteria coculture.=A0 The idea is=
 to exploit the native antibiotic peptides production that some strains of =
Lactobacillus produce as a selection factor maintaining the protein express=
ion plasmid in a second strain.=A0 At the end ideally the system would end =
up as something that could be deployed easily to 3rd world people with a yo=
gurt culturing knowledge base and some polypeptide medical need.=A0=A0 <br>
<br>A first protein expression target could be Human intrinsic factor (HIF)=
.=A0 The gut stops/slows producing it in many people around age 60 leading =
to b vitamin deficiencies that cause dementia/alzheimers like symptoms. In =
acute cases if it&#39;s detected (a big if)the approach to treating it is t=
o use animal sourced intrinsic factor.=A0 There is of course a pile of regu=
lation/industrial capital required to allow that production.=A0 Sticking it=
 in yogurt would be comparatively simple (if it works) and allow production=
 anywhere there is milk and a warm clean pot.<br>
<br>Alternately we could muck around with some apple strain selection and m=
ake the hive1tree.<br><br>-AC<br><br><br>On Thursday, July 26, 2012 5:00:56=
 PM UTC-4, Michael Horwath wrote:<blockquote class=3D"gmail_quote" style=3D=
"margin:0;margin-left:0.8ex;border-left:1px #ccc solid;padding-left:1ex">
Not yet myself, unless you count yeast culture (beer brewing :D...) but I&#=
39;d definitely be up for getting involved with an OSbio project!=A0 I&#39;=
m an immunology graduate student so I do various bio techniques as my &quot=
;day job.&quot;=A0 What kind of protein synthesis project do you have in mi=
nd?<br>

<br>Mike<br><br><div class=3D"gmail_quote">On Thu, Jul 26, 2012 at 4:40 PM,=
 Addison <span dir=3D"ltr"></span><br><blockquote class=3D"gmail_quote" sty=
le=3D"margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
Yeah, I&#39;m going to pick up some of the equipment. It&#39;ll be fun to d=
o some of the DIY bio stuff, I&#39;ve got some neat thoughts on protein syn=
thesis that should be fairly straight forward to implement with a minimal a=
mount of technology.=A0 Is anyone doing OSbio at the hackspace these days? =
<br>

<div><br>On Tuesday, July 24, 2012 5:01:59 PM UTC-4, Addison wrote:<blockqu=
ote class=3D"gmail_quote" style=3D"margin:0;margin-left:0.8ex;border-left:1=
px #ccc solid;padding-left:1ex">Hey Y&#39;all,<br><br>It&#39;s been a while=
, but this seems to be Hive13 cromulent.=A0 P&amp;G mason, cinci, st bernar=
d, and west chester are getting rid of lots of industrial/research stuff.=
=A0 The auction starts tomorrow (7/25) and ends on thursday (7/26)<br>

=A0<br>It Includes:<br>cameras (old digital/old analogue)<br>video cameras<=
br>balances<br>constant temperature baths<br>densitometers<br>incubators<br=
>switches/hubs<br>LN2 tanks<br>etc.<br><br>Here&#39;s a link: <a href=3D"ht=
tp://www.go-dove.com/en/event-16591/Procter-Gamble-Sale-21-%28North-America=
-USA-Canada%29/lots?Action=3DMove&amp;AP=3D1&amp;Auction_ID=3D0&amp;PP=3D50=
&amp;Show=3Dall&amp;EventID=3D16591&amp;LF=3DCincinnati%2C+OH+&amp;SortBy=
=3Dpaddedinventoryid&amp;SortDir=3Dasc" target=3D"_blank">http://www.go-dov=
e.com/en/<u></u>even<u></u>t-16591/Procter-Gamble-<u></u>Sale-<u></u>21-(No=
rth-America-USA-<u></u>Canada)/<u></u>lots?Action=3DMove&amp;AP=3D1&amp;<u>=
</u>Auction_<u></u>ID=3D0&amp;PP=3D50&amp;Show=3Dall&amp;<u></u>EventID=3D<=
u></u>16591&amp;LF=3DCincinnati%<u></u>2C+OH+&amp;<u></u>SortBy=3D<u></u>pa=
ddedinventoryid&amp;<u></u>SortDir=3Dasc</a><br>

<br>Good luck!<br><br></blockquote>

<p></p>

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</blockquote></div>

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