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Description:
Feedback, support, discussion, news, announcements, comments, suggestions and bug-reports concerning Blast2GO.
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Strand-specific Blast2GO?
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Dear Blast2GO community,
Is there any way to tell Blast2GO to treat nucleotide sequences strand
specifically (i.e. forward strand translations only)? Currently try to
annotate a sequence and its reverse complement, Blast2GO treats them
the same, assigns the same description and the same GO terms. This... more »
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third column in b2g4pipe output has NA
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Hi I formatted the database for blast according to the tutorial given
on blast2go page ([link]
tutorialblastall) then I ran b2g4pipe on the blast results I get the
following output in the .annot file :-
Glyma19g45090.1 GO:0016301 ---NA---
Glyma19g45090.1 GO:0009744... more »
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blast2go files
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Hi,
I have a list of .xml files from blastx. these file names do not show
the contig # as when they are in the blast 2 go GUI. I have a list
of candidate genes that I want to pull from the .xml files based on
contig number however. Can anyone suggest a quick and easy way of
doing this?... more »
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what's the difference between annotation, annotation augamentation and Go-slim
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Dear all:
I have done blastx and mapping successfully for my 17000 est sequence,
then I went on for their functional annotation under defult annotation
configuration,the process ran very fine. Now I don not know how to do
next. DO I have to do annotation augment and Go-slim? Can they
influnece the speci city and stringency? what is the difference... more »
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how to run blast search in genebank with B2Gpipeline
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Hi, I installed the b2gpipeline, I have fasta file, which needs to be
annotated via blast search in genebank. Can I run a blast search
against genebank in b2gpipe? if do, how can I change the b2gpipe
properties? Thanks.
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mapping stalls
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Dear list,
Being new to blast2go, I'm not sure whether I'm missing something so
apologies if this is a stupid question.
I've succesfully imported a set of ca 17,000 sequences from an EST
project, and blasted them resulting in blastx hits for many of them,
and saved them as xml.
Now I want to map those blast hits and set up the mapping step. It... more »
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No Sequence selected for fisher's test
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I have a problem with the fisher's test. I have sequence ID's in a
text file (separate raw), and this file works when I select multiple
sequences with Select -> Select by Sequence Name or ID. However, when
I use this same text file to select
a test set for the fisher's test, no sequences are selected.... more »
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GO Slim Results
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I have followed the tutorial steps provided, but cannot figure out how
to view the GO-Slim data. More specifically, I would like to view my
sequences/descriptions per GO-Slim category. In general, if anyone
has any suggestions as how to assign more general biological
functions, such as reproduction, eye development, etc.?... more »
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