Description:
Research into virology. (Moderated)
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Cervical Swabs for HPV PCR
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Hello,I would like to hear from anyone who is actually doing PCR for Human Papillomaviruses on DNA from cells obtained by cervical swabs, cytobrush, cervical lavage etc. Basically, what works best for you? For example, which do you recommend.. brush or swab or?,is the swab, brush etc. left dry in collection tube?, in a transport media?, at what temperature? How best... more »
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virus life
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Hello Philipp, Didi you get any reply on this question. I was too looking for the same. It would be great if you reply Thanks Ekta
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From Paul Tucker
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Dear Colleague, I'd like to remind you of the *VIZIER / SPINE2 Workshop on “Structural Virology* taking place 14th - 16th July, 2008, Vienna, Austria see: [link] We can accept up to 100 participants (on a first come first serve basis) and although we're not there yet the number is starting to... more »
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Could this be a possible treatment for HIV/AIDS?
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Could this be a possible treatment for HIV/AIDS: Synthesize a peptide nucleic acid antisense oligonucleotide for HIV’s gag gene’s mRNA. The reason for using PNA is that it is substantially more resistant to enzyme degradation by nucleases and proteases. The reason for choosing the gag gene is that it’s proteins, p24, p17, p7,... more »
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Virology Digest, Vol 29, Issue 1
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Doria, If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought...... more »
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particle to pfu ratios
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We are wondering what the particle:PFU ratio is for porcine parvovirus. Any ideas how to acquire this statistic? Doria Bowers
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VIZIER / SPINE2 Workshop on Structural Virology
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Dear Virologist, Registrations are now being accepted for the *VIZIER / SPINE2 Workshop on “Structural Virology* taking place from 14th - 16th July 2008, in Vienna, Austria This workshop will cover recent advances in our understanding of viral entry, assembly, replication and pathogenesis principally, but not... more »
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International Science and Engineering Fair - Call for Judges
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The 59th ISEF will be held this year in Atlanta, Georgia May 11th-17th. I am looking for volunteers to serve as Grand Awards Judges in the Microbiology Category. Last year's ISEF in Albuquerque, New Mexico, over 45 different countries sent 1510 secondary school finalists to compete. We expect the same turn out at this year's... more »
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TCID50
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Hi, Any recommendations. I am doing so viral replication assays under hypoxia and normoxia. This is causing my cells to grow a different rates through the time points. I am trying to nomalise the data. Should i normalise to cells at time of infection as standard. At 24 hours, for cell count at each time... more »
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