toluidine blue
At a lower acidic pH, toluidine blue gives only a blue or green colour. The
pectins at that pH do not carry negative charges since their carboxyl groups
would be complete (COOH); therefore they cannot react with the dye. Only the
lignin with its benzene rings will do so, hence the green colour. ...
Oct 9 1997 by Frederique Guinel f
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How to stain plant sections with toluidine blue
timber...@yahoo.com bionet plants education There have been several discussions
in this newsgroup about toluidine blue. They can be found at the following link:
http://groups.google.com/group/bionet.plants.education/search?hl=en&group=bionet
.plants.education&q=toluidine+blue&qt_g=Search+this+group A google search ...
Mar 27 2008 by timber...@yahoo.com
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Help!
EDU bionet plants education I found this recipe for Toluidine blue O. Add 0.05 g
toluidine blue O to 100 ml of 0.1M phosphate buffer, pH 6.8. Dilute this mixture
1:1 with distilled water before use. Nancy S. Kirkpatrick, PhD. Assistant
Professor & Chief Health Professions Advisor Biology Department Lake Superior
...
Feb 4 1999 by "Nancy S. Kirkpatrick"
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Toluidine Blue
Nancy Harrison vul...@sonic.net bionet plants education I need a recipe for this
stain, to be used to stain fresh plant tissue. I know several people have
mentioned it here - we don't have any at our lab, although we could order it
from Sigma. We're not sure whether to use the alcohol solution or the aqueous
...
Oct 8 1997 by Nancy Harrison
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Toluidine blue - thanks!
Nancy Harrison vul...@sonic.net bionet plants education Thanks everyone who
responded by post or email to my toluidine blue question. We have gathered
enough information to try several different types of solutions next semester.
Also, as a bonus, I was glad to get the subsidiary info about phloroglucinol.
...
Oct 12 1997 by Nancy Harrison
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(none)
Have a go at cutting fresh sections - stain in toluidine blue. Then relate the
tissue layout to the life style - collenchyma distribution - stellate parenchyma
etc.. You will have to admit that you don't understand all you see, but that is
no bad thing I find students wake up when they can see a `reason` behind the ...
Aug 22 1996 by Adrian Bell
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Teaching plant anatomy
Use 0.05M toluidine blue for about 30 seconds to stain the specimens. I find
celery petioles quite good for this. Are you doing plant diversity ? Take the
students out in the field to collect their own specimens rather than getting
them yourself or buying the material in. (Everybody appreciates a 'jolly', ...
Aug 28 1996 by "John Penny"
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Experience with Plant Transport
Cut stems were placed in aqueous toluidine blue O solution. I was not expecting
to observe upward movement of dye at about 1 cm per second, or to be able to so
easily see the bundles and their branching pattern with the naked eye! Thin
sections (hand microtome) revealed dye in tissue around functional tracheary ...
May 12 1997 by "Robert C. Hodson"
- 3 messages - 3 authors
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stain vascular tissue?
Wilson A. Taylor taylo...@uwec.edu bionet plants education At 04:55 PM 2/7/2007,
you wrote: Toluidine blue also works for lignified and suberized tissues, ... It
works pretty well, though lightly lignified thickenings do not stain a
particularly dark blue green (in contrast to the blue-purple of unlignified cell
...
Feb 8 2007 by Wilson A. Taylor
- 4 messages - 4 authors
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toluidine blue
I'm excited to try the tetraborate addition to see if it improves the contrast
with Tol blue...that would be nice and might convince me to switch away from
Phloroglucinol. The tol blue w/o borate is just too murky to me. ross I
partially get around the corrosive properties of HCl by having the students
destain in ...
Oct 9 1997 by "Douglas P. Jensen"
- 4 messages - 4 authors
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