Description:
Research on Zebrafish (Daniorerio) (Moderated)
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pCS2Tal3 target vectors
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Hi all
I'm looking to use pCS2Tal3 as my final target vector for my TALENs
but I've found that there are two mentioned in most protocols and on
the ADDGENE website:
pCS2TAL3DD and pCS2TAL3RR.
Does it matter which one I use? Can I use the same one for my forward
and reverse monomers or do I need to use both? If not which one would... more »
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Sheer Zebrafish available through MTA
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Dear All, I wanted to inform you of a very useful zebrafish model developed at the University of Wisconsin-Milwaukee by Dr. Henry Tomasiewicz. This triple homozygous mutant fish, known as sheer zebrafish, are characterized by their optically transparent quality throughout life. His laboratory has crossed mutants transparent (tra), albino (alb), and pfeffer (pfe) in which homozygous mutants are each defective in synthesis of different pigments (iridophores, melanin, and xanthophores respectively). Transparent zebrafish enable studies requiring the observation of cellular processes and interactions, and allow for manipulation of cells in adult fish. Sheer fish also allow for observation of all of the internal organs in the adult fish. These transparent fish are ideal for the study of fluorescent proteins, especially when studying the effects of environmental toxicants on neurodegenerative and other disease processes where optical clarity is at a premium.... more »
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roy mutant line
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HI everyone
I am trying to track down the roy mutant line, which I need to do some behavioural experiments.
Does anyone have it swimming around in their aquarium who would be willing to send it to me?
Thanks
Will
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open positions in Tübingen, Germany
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Dear zebrafish researchers,
Ph.D. and postdoctoral positions are available in my lab at the Max Planck Institute for Developmental Biology in Tï¿œbingen, Germany.
We study classical developmental biology questions with quantitative methods in zebrafish embryos. We want to understand how extracellular... more »
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Post doc position in developmental genetics - in Copenhagen
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Post doc in developmental genetics
Vacant position as Post doc in developmental genetics (around 3 years) at Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen
Our research group focuses on molecular genetics of cardiac development and congenital heart disease (CHD). The post doc project will span from identification of disease genes in patients with heart defects to investigations of molecular mechanisms involved in heart development - with focus on left-right patterning. Functional characterization of cardiac developmental genes will be performed using the zebrafish as a model organism, and the project will include implementation of methods in zebrafish for gene targeting (e.g. TALENs) and phenotypic characterization of heart defects. Thus, we exclusively seek candidates with documented expertise in zebrafish developmental biology.... more »
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Post Doc available in London-UK
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We are seeking to recruit a Postdoctoral Research Associate to join the Department of Life Sciences at Imperial College London and the Department of Structural & Molecular Biology at University College London.
The purpose of the post is to develop novel fluorescent biosensors for live cell imaging using FRET and FLIM within an Optical Projection Tomography (OPT) instrument to be developed as a biomedical imaging platform for Cancer and Inflammation. We aim to live image signalling episodes in juvenile and adult transparent zebrafish as tumours progress within their microenvironment and immune cells interact with specific stimuli that drive inflammatory responses. This presents an unprecedented opportunity for a highly motivated and creative scientist to study signaling pathways regulating the motility, migration and invasion of cells as they relate to cancer and inflammation using the state-of-the-art fluorescent biosensor tools and novel in vivo imaging modalities. It is intended that this project will provide foundation for subsequent biology-driven research projects.... more »
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Zbrafish Digest, Vol 93, Issue 3
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Hi Tyrone,
I am about to try this Ki67 antibody (Rab-Ki67 (Novocastra, 2017-01)).
I'll let you know how it goes.
Chase
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-- Environmental toxicology
University of Washington
Department of Environmental and Occupational Health Sciences
4225 Roosevelt Way NE, Suite 100
Seattle, WA 98105... more »
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Ki67 antibodies
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Hello,
Has anyone had any success with Ki67 antibodies on zebrafish tissue?
Thanks
Dr Tyrone Genade
Department of Human Biology
University of Cape Town
South Africa
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Reactome Pathway Database User Survey 2013
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Reactome is committed to providing access to high-quality pathway information and helpful data analysis and visualization tools. With this in mind, we are actively soliciting comments from the research community in order to assess community needs. We are interested to hear about your experiences with Reactome, and would like to know a bit about your background and research interests so that we can continue to improve the Reactome website and tools.... more »
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