bionet.organisms.zebrafish Google Group

amyloids, NFTs or Lewy body protocol?

michael.br...@biotec.tu-dresden.de (Michael Brand) — Thu, 06 Jun 2013 07:18:08 UT

Dear colleagues

has anyone had any luck with a staining protocol to detect amyloid deposits, neurofibrillary tangles (NFTs) or Lewy bodies in zebrafish brain ? I would be grateful for some help.

best wishes

Michael Brand
Dresden

Re: [Zbrafish] pCS2Tal3 target vectors

miriam_moria...@hotmail.com (KerryGirleen) — Wed, 22 May 2013 13:41:26 UT

Hi Richard

Thanks for clearing that up for me!

Miriam

Re: [Zbrafish] pCS2Tal3 target vectors

richard.magu...@york.ac.uk (Richard Maguire) — Thu, 09 May 2013 08:47:48 UT

Hi, Miriam.

The Grunwald lab pCS2 TAL3 destination vectors use obligate
heterodimeric FOKI domains. DD must heterodimerise with RR in order to
induce a double strand break. This reduces possible off target effects
you may otherwise get due to spurious homodimerisation of the "standard"
homodimeric FokI.

pCS2Tal3 target vectors

miriam_moria...@hotmail.com (KerryGirleen) — Wed, 08 May 2013 15:18:51 UT

Hi all

I'm looking to use pCS2Tal3 as my final target vector for my TALENs
but I've found that there are two mentioned in most protocols and on
the ADDGENE website:

pCS2TAL3DD and pCS2TAL3RR.

Does it matter which one I use? Can I use the same one for my forward
and reverse monomers or do I need to use both? If not which one would

Re: Funduscopy zebrafish

htomasiew...@gmail.com — Fri, 03 May 2013 19:40:54 UT

In addition to the melanin in the retina older fish will have a shiny reflective layer made up of the iridiphores. We have a line of fish, sheer, which lacks these pigments. For younger fish you may be able to use albinos.

Re: Zbrafish Digest, Vol 93, Issue 3

tgen...@gmail.com (Tyrone Genade) — Tue, 30 Apr 2013 10:34:51 UT

did it work?

Re: roy mutant line

davidla...@gmail.com (David Lains) — Thu, 11 Apr 2013 18:20:58 UT

Hello Will

ZIRC has the Roy as a single line in the freezers so the recovered embryos would be het/wildtype.
[link]

We also have the Roy in a double mutant with nacre in the transparent casper line.
[link]

Sheer Zebrafish available through MTA

j...@silvaggi.com — Thu, 11 Apr 2013 02:09:09 UT

Dear All,

I wanted to inform you of a very useful zebrafish model developed at the University of Wisconsin-Milwaukee by Dr. Henry Tomasiewicz. This triple homozygous mutant fish, known as sheer zebrafish, are characterized by their optically transparent quality throughout life. His laboratory has crossed mutants transparent (tra), albino (alb), and pfeffer (pfe) in which homozygous mutants are each defective in synthesis of different pigments (iridophores, melanin, and xanthophores respectively). Transparent zebrafish enable studies requiring the observation of cellular processes and interactions, and allow for manipulation of cells in adult fish. Sheer fish also allow for observation of all of the internal organs in the adult fish. These transparent fish are ideal for the study of fluorescent proteins, especially when studying the effects of environmental toxicants on neurodegenerative and other disease processes where optical clarity is at a premium.

roy mutant line

wnm...@gmail.com (Will Norton) — Thu, 11 Apr 2013 13:17:31 UT

HI everyone

I am trying to track down the roy mutant line, which I need to do some behavioural experiments.

Does anyone have it swimming around in their aquarium who would be willing to send it to me?

Thanks

Will

open positions in Tübingen, Germany

patrick.muel...@tuebingen.mpg.de (Patrick Mueller) — Sat, 06 Apr 2013 15:27:05 UT

Dear zebrafish researchers,

Ph.D. and postdoctoral positions are available in my lab at the Max
Planck Institute for Developmental Biology in Tï¿œbingen, Germany.

We study classical developmental biology questions with quantitative
methods in zebrafish embryos. We want to understand how extracellular

Re: paramecium filters

ap...@zebrafish.org (zebrafish) — Tue, 02 Apr 2013 23:44:15 UT

Hi Claudia and Aaron,
The original sizes may not be available, so I recommend using 100 micron and 20 or 10 mircon. When searching the web, look for 'bolting cloth'. The following website has several sizes:

[link]

Additional note, ZIRC has an updated paramecia culturing protocol on-line if you haven't seen it. I would be happy to answer questions, if needed.

Re: paramecium filters

ap...@zebrafish.org (zebrafish) — Tue, 02 Apr 2013 23:44:15 UT

[link]

Additional note, ZIRC has an updated paramecia culturing protocol on-line if you haven't seen it. I would be happy to answer questions, if needed.

Re: paramecium filters

amlam...@gmail.com — Tue, 02 Apr 2013 15:47:57 UT

I am looking for the same filter cloth for filtering paramecia. Do you have a recommendation of a vendor from which I could purchase sufficient filter cloth?

Thanks,
Aaron

Post doc position in developmental genetics - in Copenhagen

k.deli...@gmail.com (KdeLinde) — Tue, 02 Apr 2013 06:21:21 UT

Post doc in developmental genetics
Vacant position as Post doc in developmental genetics (around 3 years) at Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen

Our research group focuses on molecular genetics of cardiac development and congenital heart disease (CHD). The post doc project will span from identification of disease genes in patients with heart defects to investigations of molecular mechanisms involved in heart development - with focus on left-right patterning. Functional characterization of cardiac developmental genes will be performed using the zebrafish as a model organism, and the project will include implementation of methods in zebrafish for gene targeting (e.g. TALENs) and phenotypic characterization of heart defects. Thus, we exclusively seek candidates with documented expertise in zebrafish developmental biology.

Re: protocol

jle...@umich.edu (Jenny Lenkowski) — Tue, 26 Mar 2013 17:12:24 UT

Hi Beth,

We started using an alkaline lysis protocol that is quick and a lot cheaper than a proteinase K digestion. I have copied it below. We have also successfully used this for genotyping whole embryos, just with smaller volumes.

Best,
Jenny
Post-doctoral Fellow, Raymond Lab
University of Michigan