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Announcement of FOURTH Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases
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Keith Joung
Nov 20, 2012, 7:57:34 PM11/20/12
to bionet-organi...@moderators.isc.org, jjo...@partners.org
Dear Colleagues,
We are writing again to solicit recommendations for appropriate genes
to target in our FOURTH solicitation of zebrafish genes to target with
engineered nucleases and we plan to construct 96 TALEN pairs for this
new round.
As many of you know, we have previously made three solicitations to
the community regarding the construction of engineered zinc finger
pairs for endogenous zebrafish gene targets. As a result of these
efforts, out of 192 zebrafish genes we have targeted to date, we have
successfully constructed zinc finger pairs for 49 zebrafish genes and
TALEN pairs for 84 zebrafish genes with TALEN pairs for 20 more genes
to be completed in the coming weeks. Plasmids encoding these
completed zinc fingers and TALENs have been deposited with the non-
profit plasmid distribution service Addgene (http://www.addgene.org/
zfc/arrays/ for the zinc fingers and http://www.addgene.org/talengineering/TALENzebrafish/
for the TALENs). Note that the newest TALEN pairs will be available
in the coming weeks after Addgene has sequence-verified the plasmids.
Each laboratory may submit up to TWO gene targets by completing the
Excel request form (available for download here:
http://www.talengineering.org/download.htm) -- one gene recommendation
should be placed on each of the "Sheets" in the file. Place your
first choice gene on the first sheet and your second choice gene on
the second sheet. Please note that ALL required fields must be filled
out – we will not accept or consider any incomplete forms. For a gene
to be considered appropriate for this effort, we ask each recommender
to verify on the form that mutations in the gene of interest do not
already exist and that efforts to generate mutations in the
recommended gene are not underway elsewhere. VERY IMPORTANT NOTE:
Each gene submitted MUST include its Ensembl Gene ID (ENSDARG) the
Ensembl Transcript ID (ENSDART), and the gene name (e.g.-- Example:
ENSDARG00000024771, ENSDART00000033574, slc24a5). In the event that
Ensembl has incorrectly annotated the gene of interest, the full
genomic sequence of your gene should be included with exons denoted by
upper case and introns in lower case. Genes not correctly submitted in
one of these formats will NOT be considered for targeting.
Recommendations will be considered in the order in which they are
received. We will choose one gene for targeting from each lab until
we reach 96 gene targets. If we receive fewer than 96 targetable
"first choice" gene requests, we will then consider “second choice”
requests in the order in which we received the e-mails until we reach
96 gene targets. Please note that we plan to make another FIFTH
solicitation in the spring of 2013.
Please e-mail your completed form to zebraf...@zincfingers.org at
or after (and not before) 12:00 Noon (Eastern US time) November 30th,
2012. Requests received before this date/time will not be
considered. Gene recommendations will only be accepted via e-mail.
Note that recommendations from scientists at commercial entities will
not be accepted.
With best regards,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
We are writing again to solicit recommendations for appropriate genes
to target in our FOURTH solicitation of zebrafish genes to target with
engineered nucleases and we plan to construct 96 TALEN pairs for this
new round.
As many of you know, we have previously made three solicitations to
the community regarding the construction of engineered zinc finger
pairs for endogenous zebrafish gene targets. As a result of these
efforts, out of 192 zebrafish genes we have targeted to date, we have
successfully constructed zinc finger pairs for 49 zebrafish genes and
TALEN pairs for 84 zebrafish genes with TALEN pairs for 20 more genes
to be completed in the coming weeks. Plasmids encoding these
completed zinc fingers and TALENs have been deposited with the non-
profit plasmid distribution service Addgene (http://www.addgene.org/
zfc/arrays/ for the zinc fingers and http://www.addgene.org/talengineering/TALENzebrafish/
for the TALENs). Note that the newest TALEN pairs will be available
in the coming weeks after Addgene has sequence-verified the plasmids.
Each laboratory may submit up to TWO gene targets by completing the
Excel request form (available for download here:
http://www.talengineering.org/download.htm) -- one gene recommendation
should be placed on each of the "Sheets" in the file. Place your
first choice gene on the first sheet and your second choice gene on
the second sheet. Please note that ALL required fields must be filled
out – we will not accept or consider any incomplete forms. For a gene
to be considered appropriate for this effort, we ask each recommender
to verify on the form that mutations in the gene of interest do not
already exist and that efforts to generate mutations in the
recommended gene are not underway elsewhere. VERY IMPORTANT NOTE:
Each gene submitted MUST include its Ensembl Gene ID (ENSDARG) the
Ensembl Transcript ID (ENSDART), and the gene name (e.g.-- Example:
ENSDARG00000024771, ENSDART00000033574, slc24a5). In the event that
Ensembl has incorrectly annotated the gene of interest, the full
genomic sequence of your gene should be included with exons denoted by
upper case and introns in lower case. Genes not correctly submitted in
one of these formats will NOT be considered for targeting.
Recommendations will be considered in the order in which they are
received. We will choose one gene for targeting from each lab until
we reach 96 gene targets. If we receive fewer than 96 targetable
"first choice" gene requests, we will then consider “second choice”
requests in the order in which we received the e-mails until we reach
96 gene targets. Please note that we plan to make another FIFTH
solicitation in the spring of 2013.
Please e-mail your completed form to zebraf...@zincfingers.org at
or after (and not before) 12:00 Noon (Eastern US time) November 30th,
2012. Requests received before this date/time will not be
considered. Gene recommendations will only be accepted via e-mail.
Note that recommendations from scientists at commercial entities will
not be accepted.
With best regards,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
Keith Joung
Jan 6, 2013, 6:50:56 PM1/6/13
to bionet-organi...@moderators.isc.org
Dear all,
Listed below are the 96 zebrafish genes and target sites for which we
will make FLASH-assembled TALENs for the community as part of our NIH-
sponsored initiative. We hope to deposit the plasmids encoding these
TALENs with Addgene by February 2013. We will write with updates as
we make those deposits.
Best,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
TALEN # Targetsitename TALEN (5' to 3')
1 ZebrafishCommunity-aak1-left TCTTTGACTCCCGGAGAG
1 ZebrafishCommunity-aak1-right TCCCCCTCCAGAACCTGG
2 ZebrafishCommunity-amh-left TCCAGGCAAGATTTGGGC
2 ZebrafishCommunity-amh-right TACGACCCAATCGCCACG
3 ZebrafishCommunity-arf6-left TGGGGAAGATGCTTTCCA
3 ZebrafishCommunity-arf6-right TCAATATTCTCATCTCC
4 ZebrafishCommunity-arnt1-left TCCCGTCTCTGGCCATGA
4 ZebrafishCommunity-arnt1-right TGCACACCGTTGGAATGA
5 ZebrafishCommunity-atg12-left TTAATCAGTCGTTTGCGC
5 ZebrafishCommunity-atg12-right TCAAAAAGCACACCAACT
6 ZebrafishCommunity-atg5-left TAAAGTCAAGAAACATTT
6 ZebrafishCommunity-atg5-right TTCCTCCACATCCTCTGC
7 ZebrafishCommunity-avpl-left TGCTGTGTGTGCTCGCGC
7 ZebrafishCommunity-avpl-right TTCTGGATGTAGCAGGCA
8 ZebrafishCommunity-Ccl2-left TTTTCGTTTTCCACTATA
8 ZebrafishCommunity-Ccl2-right TATGATAACTCTGAACCT
9 ZebrafishCommunity-cebpb-left TGGAAGTGGCCGGTTTTT
9 ZebrafishCommunity-cebpb-right TTAGTGCTGTGGAAAGCG
10 ZebrafishCommunity-CIT1-left TGAAGTTTAAATATGGAG
10 ZebrafishCommunity-CIT1-right TCGACTGCGGACAGATCT
11 ZebrafishCommunity-cited2-left TGGTAGACCGCATGATGG
11 ZebrafishCommunity-cited2-right TTCACAGCATCTGGGAAA
12 ZebrafishCommunity-ComplementFactorH-left TGATTACATGTGAACCTC
12 ZebrafishCommunity-ComplementFactorH-right TCCAAAAGGAGGTGGACA
13 ZebrafishCommunity-crispld2-left TATCAGGAGGAGCTAGAA
13 ZebrafishCommunity-crispld2-right TTCGGACAGGAGCGTCGG
14 ZebrafishCommunity-dachc-left TCCAGACCTGGCAGACCT
14 ZebrafishCommunity-dachc-right TGTCGGGTGATGTCACAT
15 ZebrafishCommunity-dbx1b-left TCCCGTGCAAGGAATGAA
15 ZebrafishCommunity-dbx1b-right TCCGTCAAAACAAGGGAA
16 ZebrafishCommunity-DGKE-left TATGGTGTCAAACCACAG
16 ZebrafishCommunity-DGKE-right TCATCGGTCAGACTGGAA
17 ZebrafishCommunity-Dlk1-left TGGTGGCGCAGCAGAGGG
17 ZebrafishCommunity-Dlk1-right TGATGTTCACGGTGGCC
18 ZebrafishCommunity-Dlk2-left TTGGCCACGGAAAATGTG
18 ZebrafishCommunity-Dlk2-right TCCCGGATCACACCTG
19 ZebrafishCommunity-drd4b-left TTCACTTACAATGTTCCC
19 ZebrafishCommunity-drd4b-right TAACAATGATAAGCAATA
20 ZebrafishCommunity-EMP2-left TCAGGCAGTGCAGGCCAC
20 ZebrafishCommunity-EMP2-right TCCCACACAGCACAAGAT
21 ZebrafishCommunity-eps15-left TTGCCCATTGCCTATCGC
21 ZebrafishCommunity-eps15-right TAGTGTTTCTGTTCCCAC
22 ZebrafishCommunity-esr1-left TCAGTGGGATACTACCCG
22 ZebrafishCommunity-esr1-right TCTGTAGATGTTCCTCGT
23 ZebrafishCommunity-fam123b-left TGGAGATCGCCACAAGAT
23 ZebrafishCommunity-fam123b-right TCTGACGATGGACCCCTC
24 ZebrafishCommunity-fgfbp1a-left TGTCTCTCCGCGGAATTT
24 ZebrafishCommunity-fgfbp1a-right TGTGAGACGCACGCGAGA
25 ZebrafishCommunity-foxl2-left TAACGGCATGATACTCAT
25 ZebrafishCommunity-foxl2-right TCGGTCTTTCTCCGCTGA
26 ZebrafishCommunity-FoxP1b-left TCGGGGACAGAGGCGGCA
26 ZebrafishCommunity-FoxP1b-right TGGGCGGAGCGCCGTTCT
27 ZebrafishCommunity-foxp3a-left TGCTTCTTAATGCTACTG
27 ZebrafishCommunity-foxp3a-right TGACTACTCCTGTTATCA
28 ZebrafishCommunity-Frizzled5-left TCGGATTTTGGCTGCATG
28 ZebrafishCommunity-Frizzled5-right TCTCCGAGCCCAGACAGT
29 ZebrafishCommunity-Fryl-left TTCCAGGCGGTGCGTAAG
29 ZebrafishCommunity-Fryl-right TCAGCCTCAGTTCCTTCA
30 ZebrafishCommunity-fscn2a-left TAAATCACGAAAATCGAT
30 ZebrafishCommunity-fscn2a-right TTGATCTTGAAACCAAAT
31 ZebrafishCommunity-GnIH-left TGTAAGATGTCCTACTTC
31 ZebrafishCommunity-GnIH-right TGCTCAGGATGCCGAGGG
32 ZebrafishCommunity-GnRH3-left TTGGAGGTCAGTCTTTGC
32 ZebrafishCommunity-GnRH3-right TTCCACCGGGAAGCCAAC
33 ZebrafishCommunity-gper-left TGGAGGAGCAGACTACCA
33 ZebrafishCommunity-gper-right TGCTCGGTGCCATTCACA
34 ZebrafishCommunity-hemogen-site1-left TGGAAGACCCGTTGGAGA
34 ZebrafishCommunity-hemogen-site1-right TCTGAATCTTTTATTTCA
35 ZebrafishCommunity-hemogen-site2-left TGATTTGTTTGTTTGCT
35 ZebrafishCommunity-hemogen-site2-right TCTCTGTCTCTGAGTCG
36 ZebrafishCommunity-hif1al-site1-left TGTTTCAGCAGAGCCCCG
36 ZebrafishCommunity-hif1al-site1-right TCCACTCCTTCCATCTCC
37 ZebrafishCommunity-hif1al-site2-left TGCCCTCAGGACTTCTGC
37 ZebrafishCommunity-hif1al-site2-right TATTGGAGACAGAAGCT
38 ZebrafishCommunity-hirip3-left TGGCCAAAGAAAAGGACG
38 ZebrafishCommunity-hirip3-right TTCTGCAGTTGAGCCACA
39 ZebrafishCommunity-hist2h3ca1-left TCTGTTTGAGGACACCAA
39 ZebrafishCommunity-hist2h3ca1-right TGATGGTGACCCTCTTGG
40 ZebrafishCommunity-hnrnpr-left TGGATGCGGGTCTGCCCC
40 ZebrafishCommunity-hnrnpr-right TGGAAGATGTTATCCAGG
41 ZebrafishCommunity-hnrnpu-left TTAAGGTGAACGAGCTGA
41 ZebrafishCommunity-hnrnpu-right TTGTCAGAGAGCTGCCGC
42 ZebrafishCommunity-hs3st1l1-left TCTTCTGAGTTATGTGTT
42 ZebrafishCommunity-hs3st1l1-right TGGAGGAGCGGCGTATGC
43 ZebrafishCommunity-hs3st1-left TCAATCCTCTGTCCTGGT
43 ZebrafishCommunity-hs3st1-right TAGCGCAGCATCAGGCGG
44 ZebrafishCommunity-Id4-left TTTATTACAATGAAGGCC
44 ZebrafishCommunity-Id4-right TAGAAGGAAGCTTATGAG
45 ZebrafishCommunity-IPIP27A-left TGAACGAGAGGAGTGTGG
45 ZebrafishCommunity-IPIP27A-right TTGTCTGGAGGAGAGTTG
46 ZebrafishCommunity-jarid2a-left TGGGATCCCGTGGTCAGA
46 ZebrafishCommunity-jarid2a-right TGAGAGGTAGAGCACCCG
47 ZebrafishCommunity-lhx1a-left TTTATCGGACAAAGAAAC
47 ZebrafishCommunity-lhx1a-right TCCAAGGTTCTGGTCATC
48 ZebrafishCommunity-lrrtm1-left TCTAATTGGTCTGTACCT
48 ZebrafishCommunity-lrrtm1-right TATCAGCCCAGGGGGCTT
49 ZebrafishCommunity-mak-left TCAAGCAGCTGGGTGATG
49 ZebrafishCommunity-mak-right TTGCTCTTCCCCATTAGA
50 ZebrafishCommunity-mapk14a-left TCTTCTTGTGCTCTTATT
50 ZebrafishCommunity-mapk14a-right TCAGCGCCCATAAGGTGA
51 ZebrafishCommunity-max-left TGAGCGACAACGATGA
51 ZebrafishCommunity-max-right TAACTATACTCACATC
52 ZebrafishCommunity-mchr1a-left TGGCCATCTGTTTGGCCT
52 ZebrafishCommunity-mchr1a-right TGAAGGATGTAATATGGA
53 ZebrafishCommunity-mef2aa-left TGGGACGGAAGAAGATAC
53 ZebrafishCommunity-mef2aa-right TGCCGGTTCCTCTCATCC
54 ZebrafishCommunity-meis2a-left TGAGCTGGCTCACTACGG
54 ZebrafishCommunity-meis2a-right TGGATCCCCGTACATCCC
55 ZebrafishCommunity-meis4.1a-left TACGAGGATCTGGTCCAC
55 ZebrafishCommunity-meis4.1a-right TCGGAGACGGGATCCCGA
56 ZebrafishCommunity-mrc1a-left TGTTGTACAAGGGATCAG
56 ZebrafishCommunity-mrc1a-right TTGGTCCCATACACCTGC
57 ZebrafishCommunity-NPVF-left TTAGCCCTCGGCATCCTG
57 ZebrafishCommunity-NPVF-right TGAGAGCCGTAACTTCAC
58 ZebrafishCommunity-nr5a2-left TGGACTACTCCTACGACG
58 ZebrafishCommunity-nr5a2-right TCTCCGCACACTGGACAC
59 ZebrafishCommunity-nup155-left TCGCTGTCTGGCGTGTCC
59 ZebrafishCommunity-nup155-right TCAGCGCGGGACCCTGCA
60 ZebrafishCommunity-NUPL2-left TGGATAAACCCATCTCAG
60 ZebrafishCommunity-NUPL2-right TGCCCCCTGCTGACGAGG
61 ZebrafishCommunity-ovol1-left TGTTCCAGTTCCAGCGCA
61 ZebrafishCommunity-ovol1-right TGCTCGCTGTGACATTTC
62 ZebrafishCommunity-oxtr-left TTCAAGGATCAAGACTTT
62 ZebrafishCommunity-oxtrl-left TGATCCTCTGGCCCATTA
63 ZebrafishCommunity-oxtrl-right TCGTTTCCCCTACTTGAG
63 ZebrafishCommunity-oxtr-right TGGTGGCGTTTCTGGATG
64 ZebrafishCommunity-park2-left TGATCGTTTTCGTGCGGT
64 ZebrafishCommunity-park2-right TCCAACTCCACCGGGAAC
65 ZebrafishCommunity-pcolce2b-left TCCTCACATTTCGTTTCC
65 ZebrafishCommunity-pcolce2b-right TCGTACCGACACAGGTTG
66 ZebrafishCommunity-pink1-left TTTCCTCTCATAGTCAAT
66 ZebrafishCommunity-pink1-right TTTACTGACATTTTGAGC
67 ZebrafishCommunity-Popdc2-left TGAATTGGAGACAGCAGA
67 ZebrafishCommunity-Popdc2-right TCAATGAAGAGTCGCCGC
68 ZebrafishCommunity-Popdc3-left TCTCACTGTTAAGATTAT
68 ZebrafishCommunity-Popdc3-right TATTCTCAGGTAAAAGAT
69 ZebrafishCommunity-prothymosinalphaa-left TTATCATTCGCATCTCGT
69 ZebrafishCommunity-prothymosinalphaa-right TGGGGTCTCGGAATAAAA
70 ZebrafishCommunity-prrx1b-left TAATACACTTGCCAATTT
70 ZebrafishCommunity-prrx1b-right TGCATAGCTAGAAGTCAT
71 ZebrafishCommunity-PurinergicreceptorY2-left TTCAACGCATCAGTGAAT
71 ZebrafishCommunity-PurinergicreceptorY2-right TGCTGCAGCTGTGTGCAG
72 ZebrafishCommunity-rassf7b-left TGAAGGTGTGGGTGGATG
72 ZebrafishCommunity-rassf7b-right TCTTCTGAGAGGCCACAG
73 ZebrafishCommunity-scrt2-left TTTTCTGGTGCGTCTGTG
73 ZebrafishCommunity-scrt2-right TCACCAGAAACGAGCGAG
74 ZebrafishCommunity-sema6dl-left TGCTGATACTCCTGCTGG
74 ZebrafishCommunity-sema6dl-right TCAGGAAAGCTCACAGCA
75 ZebrafishCommunity-Semaphorin6bb-left TTGAAAAGTGCTGAGAAA
75 ZebrafishCommunity-Semaphorin6bb-right TTTGTCTCCTAAATCAAA
76 ZebrafishCommunity-si:ch211-120j21.1-left TGAACTGTCAGTCGGGAC
76 ZebrafishCommunity-si:ch211-120j21.1-right TGAAAGAAAGCGCATAAA
77 ZebrafishCommunity-slc50a1-left TTTCTGCAGCTGCTATCA
77 ZebrafishCommunity-slc50a1-right TGAACATCCCGACGGTGA
78 ZebrafishCommunity-snrnp35-left TCCGGTGGCGAAGGTGTA
78 ZebrafishCommunity-snrnp35-right TGTGCCGTCAATGCTGCC
79 ZebrafishCommunity-snrnp48-left TGTGTGAAACATCGTTTG
79 ZebrafishCommunity-snrnp48-right TGAAGACACTGCATGCGC
80 ZebrafishCommunity-sox1a-left TATAGCATGATGATGGAA
80 ZebrafishCommunity-sox1a-right TGTTGGTTTGGGGTCCCG
81 ZebrafishCommunity-Sox2-left TGGTGGGGTAGACTTTCG
81 ZebrafishCommunity-Sox2-right TTTCCATCATGTTATACA
82 ZebrafishCommunity-Tac3b-left TCCACGTGCTGCTGCTGC
82 ZebrafishCommunity-Tac3b-right TCGAGGGCGCTCCGCGAG
83 ZebrafishCommunity-taok2b-left TGCGGGCTGGGAGTCTCA
83 ZebrafishCommunity-taok2b-right TCCTTGTAAAAAAGTTCC
84 ZebrafishCommunity-tcof-left TAAAGACTCCTCCACCAG
84 ZebrafishCommunity-tcof-right TCACTACTGGACACTGCA
85 ZebrafishCommunity-tet2(ENSDART00000113020)-left TCAAGACCAGATACTATC
85 ZebrafishCommunity-tet2(ENSDART00000113020)-right
TTCAGTAGGTGGTAGCTG
86 ZebrafishCommunity-tet3(ENSDART00000137355)-left TTGCTCCTTGGTTGCATC
86 ZebrafishCommunity-tet3(ENSDART00000137355)-right
TAATGAAGAAAATGTTTG
87 ZebrafishCommunity-tfe3a-left TAAAATAGCCAGTCAGA
87 ZebrafishCommunity-tfe3a-right TGAGGGCTGTGGTGGG
88 ZebrafishCommunity-tfe3b-left TCGCTCAGCTCCGCCCT
88 ZebrafishCommunity-tfe3b-right TGGCAGCGAGACGGAGAT
89 ZebrafishCommunity-tnfb-left TGTCGGGGGAGTTTATCA
89 ZebrafishCommunity-tnfb-right TGAAGAATTAACCGGTAC
90 ZebrafishCommunity-TRABD2A-left TCAGCTGGAGCGCTTTTC
90 ZebrafishCommunity-TRABD2A-right TTTGCTCTCACCATGAGT
91 ZebrafishCommunity-TTC19-left TGCCAGGAGGAGCAGGGA
91 ZebrafishCommunity-TTC19-right TCACTCATCAGGACCAGC
92 ZebrafishCommunity-Vangl1-left TTCTCTGCCCAGGATAG
92 ZebrafishCommunity-Vangl1-right TGTCAGTAAACCCAGC
93 ZebrafishCommunity-vasa-left TGACTGGGAGGAAGATCA
93 ZebrafishCommunity-vasa-right TCCAAATCCTGAGCTGCA
94 ZebrafishCommunity-Vrk2-left TGTACTTGGCTGACTATG
94 ZebrafishCommunity-Vrk2-right TGCTCGCCATTAGGACAG
95 ZebrafishCommunity-ywhai-left TACAAGAATGTGGTGGGA
95 ZebrafishCommunity-ywhai-right TGCTGGACACTACCCTCC
96 ZebrafishCommunity-zmynd11-left TATGTTTGGTCGGCCATC
96 ZebrafishCommunity-zmynd11-right TGTTGGCGATTTGTTTCT
Listed below are the 96 zebrafish genes and target sites for which we
will make FLASH-assembled TALENs for the community as part of our NIH-
sponsored initiative. We hope to deposit the plasmids encoding these
TALENs with Addgene by February 2013. We will write with updates as
we make those deposits.
Best,
Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital
1 ZebrafishCommunity-aak1-left TCTTTGACTCCCGGAGAG
1 ZebrafishCommunity-aak1-right TCCCCCTCCAGAACCTGG
2 ZebrafishCommunity-amh-left TCCAGGCAAGATTTGGGC
2 ZebrafishCommunity-amh-right TACGACCCAATCGCCACG
3 ZebrafishCommunity-arf6-left TGGGGAAGATGCTTTCCA
3 ZebrafishCommunity-arf6-right TCAATATTCTCATCTCC
4 ZebrafishCommunity-arnt1-left TCCCGTCTCTGGCCATGA
4 ZebrafishCommunity-arnt1-right TGCACACCGTTGGAATGA
5 ZebrafishCommunity-atg12-left TTAATCAGTCGTTTGCGC
5 ZebrafishCommunity-atg12-right TCAAAAAGCACACCAACT
6 ZebrafishCommunity-atg5-left TAAAGTCAAGAAACATTT
6 ZebrafishCommunity-atg5-right TTCCTCCACATCCTCTGC
7 ZebrafishCommunity-avpl-left TGCTGTGTGTGCTCGCGC
7 ZebrafishCommunity-avpl-right TTCTGGATGTAGCAGGCA
8 ZebrafishCommunity-Ccl2-left TTTTCGTTTTCCACTATA
8 ZebrafishCommunity-Ccl2-right TATGATAACTCTGAACCT
9 ZebrafishCommunity-cebpb-left TGGAAGTGGCCGGTTTTT
9 ZebrafishCommunity-cebpb-right TTAGTGCTGTGGAAAGCG
10 ZebrafishCommunity-CIT1-left TGAAGTTTAAATATGGAG
10 ZebrafishCommunity-CIT1-right TCGACTGCGGACAGATCT
11 ZebrafishCommunity-cited2-left TGGTAGACCGCATGATGG
11 ZebrafishCommunity-cited2-right TTCACAGCATCTGGGAAA
12 ZebrafishCommunity-ComplementFactorH-left TGATTACATGTGAACCTC
12 ZebrafishCommunity-ComplementFactorH-right TCCAAAAGGAGGTGGACA
13 ZebrafishCommunity-crispld2-left TATCAGGAGGAGCTAGAA
13 ZebrafishCommunity-crispld2-right TTCGGACAGGAGCGTCGG
14 ZebrafishCommunity-dachc-left TCCAGACCTGGCAGACCT
14 ZebrafishCommunity-dachc-right TGTCGGGTGATGTCACAT
15 ZebrafishCommunity-dbx1b-left TCCCGTGCAAGGAATGAA
15 ZebrafishCommunity-dbx1b-right TCCGTCAAAACAAGGGAA
16 ZebrafishCommunity-DGKE-left TATGGTGTCAAACCACAG
16 ZebrafishCommunity-DGKE-right TCATCGGTCAGACTGGAA
17 ZebrafishCommunity-Dlk1-left TGGTGGCGCAGCAGAGGG
17 ZebrafishCommunity-Dlk1-right TGATGTTCACGGTGGCC
18 ZebrafishCommunity-Dlk2-left TTGGCCACGGAAAATGTG
18 ZebrafishCommunity-Dlk2-right TCCCGGATCACACCTG
19 ZebrafishCommunity-drd4b-left TTCACTTACAATGTTCCC
19 ZebrafishCommunity-drd4b-right TAACAATGATAAGCAATA
20 ZebrafishCommunity-EMP2-left TCAGGCAGTGCAGGCCAC
20 ZebrafishCommunity-EMP2-right TCCCACACAGCACAAGAT
21 ZebrafishCommunity-eps15-left TTGCCCATTGCCTATCGC
21 ZebrafishCommunity-eps15-right TAGTGTTTCTGTTCCCAC
22 ZebrafishCommunity-esr1-left TCAGTGGGATACTACCCG
22 ZebrafishCommunity-esr1-right TCTGTAGATGTTCCTCGT
23 ZebrafishCommunity-fam123b-left TGGAGATCGCCACAAGAT
23 ZebrafishCommunity-fam123b-right TCTGACGATGGACCCCTC
24 ZebrafishCommunity-fgfbp1a-left TGTCTCTCCGCGGAATTT
24 ZebrafishCommunity-fgfbp1a-right TGTGAGACGCACGCGAGA
25 ZebrafishCommunity-foxl2-left TAACGGCATGATACTCAT
25 ZebrafishCommunity-foxl2-right TCGGTCTTTCTCCGCTGA
26 ZebrafishCommunity-FoxP1b-left TCGGGGACAGAGGCGGCA
26 ZebrafishCommunity-FoxP1b-right TGGGCGGAGCGCCGTTCT
27 ZebrafishCommunity-foxp3a-left TGCTTCTTAATGCTACTG
27 ZebrafishCommunity-foxp3a-right TGACTACTCCTGTTATCA
28 ZebrafishCommunity-Frizzled5-left TCGGATTTTGGCTGCATG
28 ZebrafishCommunity-Frizzled5-right TCTCCGAGCCCAGACAGT
29 ZebrafishCommunity-Fryl-left TTCCAGGCGGTGCGTAAG
29 ZebrafishCommunity-Fryl-right TCAGCCTCAGTTCCTTCA
30 ZebrafishCommunity-fscn2a-left TAAATCACGAAAATCGAT
30 ZebrafishCommunity-fscn2a-right TTGATCTTGAAACCAAAT
31 ZebrafishCommunity-GnIH-left TGTAAGATGTCCTACTTC
31 ZebrafishCommunity-GnIH-right TGCTCAGGATGCCGAGGG
32 ZebrafishCommunity-GnRH3-left TTGGAGGTCAGTCTTTGC
32 ZebrafishCommunity-GnRH3-right TTCCACCGGGAAGCCAAC
33 ZebrafishCommunity-gper-left TGGAGGAGCAGACTACCA
33 ZebrafishCommunity-gper-right TGCTCGGTGCCATTCACA
34 ZebrafishCommunity-hemogen-site1-left TGGAAGACCCGTTGGAGA
34 ZebrafishCommunity-hemogen-site1-right TCTGAATCTTTTATTTCA
35 ZebrafishCommunity-hemogen-site2-left TGATTTGTTTGTTTGCT
35 ZebrafishCommunity-hemogen-site2-right TCTCTGTCTCTGAGTCG
36 ZebrafishCommunity-hif1al-site1-left TGTTTCAGCAGAGCCCCG
36 ZebrafishCommunity-hif1al-site1-right TCCACTCCTTCCATCTCC
37 ZebrafishCommunity-hif1al-site2-left TGCCCTCAGGACTTCTGC
37 ZebrafishCommunity-hif1al-site2-right TATTGGAGACAGAAGCT
38 ZebrafishCommunity-hirip3-left TGGCCAAAGAAAAGGACG
38 ZebrafishCommunity-hirip3-right TTCTGCAGTTGAGCCACA
39 ZebrafishCommunity-hist2h3ca1-left TCTGTTTGAGGACACCAA
39 ZebrafishCommunity-hist2h3ca1-right TGATGGTGACCCTCTTGG
40 ZebrafishCommunity-hnrnpr-left TGGATGCGGGTCTGCCCC
40 ZebrafishCommunity-hnrnpr-right TGGAAGATGTTATCCAGG
41 ZebrafishCommunity-hnrnpu-left TTAAGGTGAACGAGCTGA
41 ZebrafishCommunity-hnrnpu-right TTGTCAGAGAGCTGCCGC
42 ZebrafishCommunity-hs3st1l1-left TCTTCTGAGTTATGTGTT
42 ZebrafishCommunity-hs3st1l1-right TGGAGGAGCGGCGTATGC
43 ZebrafishCommunity-hs3st1-left TCAATCCTCTGTCCTGGT
43 ZebrafishCommunity-hs3st1-right TAGCGCAGCATCAGGCGG
44 ZebrafishCommunity-Id4-left TTTATTACAATGAAGGCC
44 ZebrafishCommunity-Id4-right TAGAAGGAAGCTTATGAG
45 ZebrafishCommunity-IPIP27A-left TGAACGAGAGGAGTGTGG
45 ZebrafishCommunity-IPIP27A-right TTGTCTGGAGGAGAGTTG
46 ZebrafishCommunity-jarid2a-left TGGGATCCCGTGGTCAGA
46 ZebrafishCommunity-jarid2a-right TGAGAGGTAGAGCACCCG
47 ZebrafishCommunity-lhx1a-left TTTATCGGACAAAGAAAC
47 ZebrafishCommunity-lhx1a-right TCCAAGGTTCTGGTCATC
48 ZebrafishCommunity-lrrtm1-left TCTAATTGGTCTGTACCT
48 ZebrafishCommunity-lrrtm1-right TATCAGCCCAGGGGGCTT
49 ZebrafishCommunity-mak-left TCAAGCAGCTGGGTGATG
49 ZebrafishCommunity-mak-right TTGCTCTTCCCCATTAGA
50 ZebrafishCommunity-mapk14a-left TCTTCTTGTGCTCTTATT
50 ZebrafishCommunity-mapk14a-right TCAGCGCCCATAAGGTGA
51 ZebrafishCommunity-max-left TGAGCGACAACGATGA
51 ZebrafishCommunity-max-right TAACTATACTCACATC
52 ZebrafishCommunity-mchr1a-left TGGCCATCTGTTTGGCCT
52 ZebrafishCommunity-mchr1a-right TGAAGGATGTAATATGGA
53 ZebrafishCommunity-mef2aa-left TGGGACGGAAGAAGATAC
53 ZebrafishCommunity-mef2aa-right TGCCGGTTCCTCTCATCC
54 ZebrafishCommunity-meis2a-left TGAGCTGGCTCACTACGG
54 ZebrafishCommunity-meis2a-right TGGATCCCCGTACATCCC
55 ZebrafishCommunity-meis4.1a-left TACGAGGATCTGGTCCAC
55 ZebrafishCommunity-meis4.1a-right TCGGAGACGGGATCCCGA
56 ZebrafishCommunity-mrc1a-left TGTTGTACAAGGGATCAG
56 ZebrafishCommunity-mrc1a-right TTGGTCCCATACACCTGC
57 ZebrafishCommunity-NPVF-left TTAGCCCTCGGCATCCTG
57 ZebrafishCommunity-NPVF-right TGAGAGCCGTAACTTCAC
58 ZebrafishCommunity-nr5a2-left TGGACTACTCCTACGACG
58 ZebrafishCommunity-nr5a2-right TCTCCGCACACTGGACAC
59 ZebrafishCommunity-nup155-left TCGCTGTCTGGCGTGTCC
59 ZebrafishCommunity-nup155-right TCAGCGCGGGACCCTGCA
60 ZebrafishCommunity-NUPL2-left TGGATAAACCCATCTCAG
60 ZebrafishCommunity-NUPL2-right TGCCCCCTGCTGACGAGG
61 ZebrafishCommunity-ovol1-left TGTTCCAGTTCCAGCGCA
61 ZebrafishCommunity-ovol1-right TGCTCGCTGTGACATTTC
62 ZebrafishCommunity-oxtr-left TTCAAGGATCAAGACTTT
62 ZebrafishCommunity-oxtrl-left TGATCCTCTGGCCCATTA
63 ZebrafishCommunity-oxtrl-right TCGTTTCCCCTACTTGAG
63 ZebrafishCommunity-oxtr-right TGGTGGCGTTTCTGGATG
64 ZebrafishCommunity-park2-left TGATCGTTTTCGTGCGGT
64 ZebrafishCommunity-park2-right TCCAACTCCACCGGGAAC
65 ZebrafishCommunity-pcolce2b-left TCCTCACATTTCGTTTCC
65 ZebrafishCommunity-pcolce2b-right TCGTACCGACACAGGTTG
66 ZebrafishCommunity-pink1-left TTTCCTCTCATAGTCAAT
66 ZebrafishCommunity-pink1-right TTTACTGACATTTTGAGC
67 ZebrafishCommunity-Popdc2-left TGAATTGGAGACAGCAGA
67 ZebrafishCommunity-Popdc2-right TCAATGAAGAGTCGCCGC
68 ZebrafishCommunity-Popdc3-left TCTCACTGTTAAGATTAT
68 ZebrafishCommunity-Popdc3-right TATTCTCAGGTAAAAGAT
69 ZebrafishCommunity-prothymosinalphaa-left TTATCATTCGCATCTCGT
69 ZebrafishCommunity-prothymosinalphaa-right TGGGGTCTCGGAATAAAA
70 ZebrafishCommunity-prrx1b-left TAATACACTTGCCAATTT
70 ZebrafishCommunity-prrx1b-right TGCATAGCTAGAAGTCAT
71 ZebrafishCommunity-PurinergicreceptorY2-left TTCAACGCATCAGTGAAT
71 ZebrafishCommunity-PurinergicreceptorY2-right TGCTGCAGCTGTGTGCAG
72 ZebrafishCommunity-rassf7b-left TGAAGGTGTGGGTGGATG
72 ZebrafishCommunity-rassf7b-right TCTTCTGAGAGGCCACAG
73 ZebrafishCommunity-scrt2-left TTTTCTGGTGCGTCTGTG
73 ZebrafishCommunity-scrt2-right TCACCAGAAACGAGCGAG
74 ZebrafishCommunity-sema6dl-left TGCTGATACTCCTGCTGG
74 ZebrafishCommunity-sema6dl-right TCAGGAAAGCTCACAGCA
75 ZebrafishCommunity-Semaphorin6bb-left TTGAAAAGTGCTGAGAAA
75 ZebrafishCommunity-Semaphorin6bb-right TTTGTCTCCTAAATCAAA
76 ZebrafishCommunity-si:ch211-120j21.1-left TGAACTGTCAGTCGGGAC
76 ZebrafishCommunity-si:ch211-120j21.1-right TGAAAGAAAGCGCATAAA
77 ZebrafishCommunity-slc50a1-left TTTCTGCAGCTGCTATCA
77 ZebrafishCommunity-slc50a1-right TGAACATCCCGACGGTGA
78 ZebrafishCommunity-snrnp35-left TCCGGTGGCGAAGGTGTA
78 ZebrafishCommunity-snrnp35-right TGTGCCGTCAATGCTGCC
79 ZebrafishCommunity-snrnp48-left TGTGTGAAACATCGTTTG
79 ZebrafishCommunity-snrnp48-right TGAAGACACTGCATGCGC
80 ZebrafishCommunity-sox1a-left TATAGCATGATGATGGAA
80 ZebrafishCommunity-sox1a-right TGTTGGTTTGGGGTCCCG
81 ZebrafishCommunity-Sox2-left TGGTGGGGTAGACTTTCG
81 ZebrafishCommunity-Sox2-right TTTCCATCATGTTATACA
82 ZebrafishCommunity-Tac3b-left TCCACGTGCTGCTGCTGC
82 ZebrafishCommunity-Tac3b-right TCGAGGGCGCTCCGCGAG
83 ZebrafishCommunity-taok2b-left TGCGGGCTGGGAGTCTCA
83 ZebrafishCommunity-taok2b-right TCCTTGTAAAAAAGTTCC
84 ZebrafishCommunity-tcof-left TAAAGACTCCTCCACCAG
84 ZebrafishCommunity-tcof-right TCACTACTGGACACTGCA
85 ZebrafishCommunity-tet2(ENSDART00000113020)-left TCAAGACCAGATACTATC
85 ZebrafishCommunity-tet2(ENSDART00000113020)-right
TTCAGTAGGTGGTAGCTG
86 ZebrafishCommunity-tet3(ENSDART00000137355)-left TTGCTCCTTGGTTGCATC
86 ZebrafishCommunity-tet3(ENSDART00000137355)-right
TAATGAAGAAAATGTTTG
87 ZebrafishCommunity-tfe3a-left TAAAATAGCCAGTCAGA
87 ZebrafishCommunity-tfe3a-right TGAGGGCTGTGGTGGG
88 ZebrafishCommunity-tfe3b-left TCGCTCAGCTCCGCCCT
88 ZebrafishCommunity-tfe3b-right TGGCAGCGAGACGGAGAT
89 ZebrafishCommunity-tnfb-left TGTCGGGGGAGTTTATCA
89 ZebrafishCommunity-tnfb-right TGAAGAATTAACCGGTAC
90 ZebrafishCommunity-TRABD2A-left TCAGCTGGAGCGCTTTTC
90 ZebrafishCommunity-TRABD2A-right TTTGCTCTCACCATGAGT
91 ZebrafishCommunity-TTC19-left TGCCAGGAGGAGCAGGGA
91 ZebrafishCommunity-TTC19-right TCACTCATCAGGACCAGC
92 ZebrafishCommunity-Vangl1-left TTCTCTGCCCAGGATAG
92 ZebrafishCommunity-Vangl1-right TGTCAGTAAACCCAGC
93 ZebrafishCommunity-vasa-left TGACTGGGAGGAAGATCA
93 ZebrafishCommunity-vasa-right TCCAAATCCTGAGCTGCA
94 ZebrafishCommunity-Vrk2-left TGTACTTGGCTGACTATG
94 ZebrafishCommunity-Vrk2-right TGCTCGCCATTAGGACAG
95 ZebrafishCommunity-ywhai-left TACAAGAATGTGGTGGGA
95 ZebrafishCommunity-ywhai-right TGCTGGACACTACCCTCC
96 ZebrafishCommunity-zmynd11-left TATGTTTGGTCGGCCATC
96 ZebrafishCommunity-zmynd11-right TGTTGGCGATTTGTTTCT
Keith Joung
Feb 28, 2013, 9:41:23 PM2/28/13
to bionet-organi...@moderators.isc.org
Dear Colleagues,
I'm pleased to provide an update on our construction of engineered
TALENs for our FOURTH NIH-sponsored solicitation of zebrafish gene
targets. We now have sequence-verified plasmids encoding TALEN pairs
for 94 of the 96 genes we targeted. The names of these 94 genes are
listed below. We are still awaiting plasmids for the NPVF and oxtr
genes and hope to provide an update on these shortly. All of these
plasmids will be deposited with Addgene in the coming days and, after
they pass Addgene's quality control checks, will be made available
here: http://www.addgene.org/talengineering/TALENzebrafish/. The
deposit of these new TALEN pairs will bring the total number of
zebrafish genes targeted with engineered nucleases by this effort to
nearly 250.
An announcement regarding the next 96 genes to be targeted in our
FIFTH solicitation will be made in the coming days.
With best regards,
Keith Joung
Randy Peterson
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander
Massachusetts General Hospital
94 new genes for which TALENs have been completed:
aak1(1of2)
amh
arf6
arnt1
atg12
atg5
avpl
Ccl2
cebpb
CIT1
cited2
ComplementFactorH
crispld2
dachc
dbx1b
DGKE
Dlk1
Dlk2
drd4b
EMP2
eps15
esr1
fam123b
fgfbp1a
foxl2
FoxP1b
foxp3a
Frizzled5
Fryl
fscn2a
GnIH
GnRH3
gper
hemogen-site1
hemogen-site2
hif1al-site1
hif1al-site2
hirip3
hist2h3ca1
hnrnpr
hnrnpu
hs3st1
hs3st1l1
Id4
IPIP27A
jarid2a
lhx1a
lrrtm1
mak
mapk14a
max
mchr1a
mef2aa
meis2a
meis4.1a
mrc1a
nr5a2
nup155
NUPL2
ovol1
oxtrl
park2
pcolce2b
pink1
Popdc2
Popdc3
prothymosinalphaa
prrx1b
PurinergicreceptorY2
rassf7b
scrt2
sema6dl
Semaphorin6bb
si:ch211-120j21.1
slc50a1
snrnp35
snrnp48
sox1a
Sox2
Tac3b
taok2b
tcof
tet2(ENSDART00000113020)
tet3(ENSDART00000137355)
tfe3a
tfe3b
tnfb
TRABD2A
TTC19
Vangl1
vasa
Vrk2
ywhai
zmynd11
I'm pleased to provide an update on our construction of engineered
TALENs for our FOURTH NIH-sponsored solicitation of zebrafish gene
targets. We now have sequence-verified plasmids encoding TALEN pairs
for 94 of the 96 genes we targeted. The names of these 94 genes are
listed below. We are still awaiting plasmids for the NPVF and oxtr
genes and hope to provide an update on these shortly. All of these
plasmids will be deposited with Addgene in the coming days and, after
they pass Addgene's quality control checks, will be made available
here: http://www.addgene.org/talengineering/TALENzebrafish/. The
deposit of these new TALEN pairs will bring the total number of
zebrafish genes targeted with engineered nucleases by this effort to
nearly 250.
An announcement regarding the next 96 genes to be targeted in our
FIFTH solicitation will be made in the coming days.
With best regards,
Keith Joung
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander
Massachusetts General Hospital
94 new genes for which TALENs have been completed:
aak1(1of2)
amh
arf6
arnt1
atg12
atg5
avpl
Ccl2
cebpb
CIT1
cited2
ComplementFactorH
crispld2
dachc
dbx1b
DGKE
Dlk1
Dlk2
drd4b
EMP2
eps15
esr1
fam123b
fgfbp1a
foxl2
FoxP1b
foxp3a
Frizzled5
Fryl
fscn2a
GnIH
GnRH3
gper
hemogen-site1
hemogen-site2
hif1al-site1
hif1al-site2
hirip3
hist2h3ca1
hnrnpr
hnrnpu
hs3st1
hs3st1l1
Id4
IPIP27A
jarid2a
lhx1a
lrrtm1
mak
mapk14a
max
mchr1a
mef2aa
meis2a
meis4.1a
mrc1a
nr5a2
nup155
NUPL2
ovol1
oxtrl
park2
pcolce2b
pink1
Popdc2
Popdc3
prothymosinalphaa
prrx1b
PurinergicreceptorY2
rassf7b
scrt2
sema6dl
Semaphorin6bb
si:ch211-120j21.1
slc50a1
snrnp35
snrnp48
sox1a
Sox2
Tac3b
taok2b
tcof
tet2(ENSDART00000113020)
tet3(ENSDART00000137355)
tfe3a
tfe3b
tnfb
TRABD2A
TTC19
Vangl1
vasa
Vrk2
ywhai
zmynd11
Keith Joung
Mar 3, 2013, 9:14:03 PM3/3/13
to bionet-organi...@moderators.isc.org, ja...@addgene.org
Dear all,
I'm pleased to announce that we have now completed construction of
sequence-verified plasmids encoding TALEN pairs for all 96 endogenous
zebrafish genes targeted in our FOURTH NIH-sponsored solicitation.
These plasmids will be deposited tomorrow with Addgene and should
become available in the next few weeks (and will be available here:
http://www.addgene.org/talengineering/TALENzebrafish/). You can also
e-mail Jason Niehaus from Addgene (ja...@addgene.org) if you wish to
be notified when a particular pair becomes available.
I'm pleased to announce that we have now completed construction of
sequence-verified plasmids encoding TALEN pairs for all 96 endogenous
zebrafish genes targeted in our FOURTH NIH-sponsored solicitation.
These plasmids will be deposited tomorrow with Addgene and should
become available in the next few weeks (and will be available here:
http://www.addgene.org/talengineering/TALENzebrafish/). You can also
e-mail Jason Niehaus from Addgene (ja...@addgene.org) if you wish to
be notified when a particular pair becomes available.
Jonathan Knight
Mar 4, 2013, 1:53:16 PM3/4/13
to zbra...@magpie.bio.indiana.edu
Dear Colleagues,
I'm pleased to provide an update on our construction of engineered
TALENs for our FOURTH NIH-sponsored solicitation of zebrafish gene
I'm pleased to provide an update on our construction of engineered
TALENs for our FOURTH NIH-sponsored solicitation of zebrafish gene
targets. We now have sequence-verified plasmids encoding TALEN pairs
for 94 of the 96 genes we targeted. The names of these 94 genes are
listed below. We are still awaiting plasmids for the NPVF and oxtr
genes and hope to provide an update on these shortly. All of these
plasmids will be deposited with Addgene in the coming days and, after
they pass Addgene's quality control checks, will be made available
here: http://www.addgene.org/talengineering/TALENzebrafish/. The
deposit of these new TALEN pairs will bring the total number of
zebrafish genes targeted with engineered nucleases by this effort to
nearly 250.
An announcement regarding the next 96 genes to be targeted in our
FIFTH solicitation will be made in the coming days.
With best regards,
Keith Joung
Randy Peterson
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander
Keith Joung
Randy Peterson
Joanna Yeh
Jon Foley
Deepak Reyon
Jeff Sander
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