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About embryo floating problem
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Journey
Nov 17, 2009, 4:44:31 PM11/17/09
to zbra...@magpie.bio.indiana.edu
Sorry for the bothering!
After the overnight hybridization when the embryos are transferred from Hyb to 2*SSC the embryos just start floating on the buffer. I tried to drop some buffer using pipets to immerse them into the water but when you changed the buffer the embryos are just floating again.
Some of these embryos was not treated with proteinase K but they have the same problem. So I think it is not because of over digestion.
Does anybody have the same problem before? How could you avoid this from happening?
Thanks a lot for your help!
Journey.
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