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protocol needed for phospho-smad staining

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Chunyue

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Sep 29, 2009, 8:10:46 PM9/29/09

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Hi All,

I tried to do immunohistochemistry using phospho-smad antibody
(purchased from Cell Signal Technology). After several trials, I
couldn't get any staining. Is anybody willing to share their
protocols?

BTW: I prefer to do the staining on whole-mount or vibrotome sections.

Thanks a lot!

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