ketamin/xylazine?
saif
im using ketamine/xylazine for 2vo surgery in male sprogue dawely rat, I lose a lot of my rats post-operatively, the problem how much time dose the rat require to have adequate temperature(isnt it within 4-5 hours postoperatively), and about the fluid supplement is recommended to give all the rats 10 ml normal saline.
dr.saif master researcher.
thank you
Michael Smeltzer
I'm not sure what 2vo refers to, but I've done quite a few surgeries on
rats. However, the best person to consult in the matter would be a
Veterinary Technician or a Veterinarian if you have access to one.
Rats should be able to push themselves up (and have control over their body
temperature) about 4-5 hours after the last dose of anesthesia. And 5-10ml
of Lactated Ringer's saline (0.9% NaCl) is also typical.
If your rats aren't waking up at all, some things to consider in your case
could be:
Anesthisia
-Are the rats receiving the correct dose of anesthesia before surgery
(should be about 40-80mg/kg Ketamine with 5-10mg/kg Xylazine)?
-And if your surgery lasts more than an hour, are they receiving the correct
maintainance doses (maintanance doses are lower, usually the inital volume
multiplied by about 0.38)?
Temperature Maintanance
-Are they kept warm (but not too warm) during the surgery?
Blood loss
-Are the rats bleeding a lot during your surgery, and is the bleeding
stopped before the end of surgery?
Hope this helps,
Mike Smeltzer
> _______________________________________________
> Neur-sci mailing list
> Neur...@net.bio.net
> http://www.bio.net/biomail/listinfo/neur-sci
>