Google Groups no longer supports new Usenet posts or subscriptions. Historical content remains viewable.
Dismiss
neuronal cultures
0 views
Skip to first unread message
letizia polito
Nov 13, 2009, 3:44:16 PM11/13/09
to Neur...@magpie.bio.indiana.edu
Hi,
does anyone work with hippocampal or cortical adult neurons? What are the
main problems vs embrional or postnatal cultures? It is difficult to have a
neuronal preparation not contaminated by microglia?
Thank you everyone.
does anyone work with hippocampal or cortical adult neurons? What are the
main problems vs embrional or postnatal cultures? It is difficult to have a
neuronal preparation not contaminated by microglia?
Thank you everyone.
Bill
Nov 18, 2009, 7:42:53 PM11/18/09
to
Adult cortical neuronal cell culture? Yes, it is difficult to get any
live neurons in your cell culture. We always work with either E16-17
or P1-3. For adult neuronal culture you need to do all kinds of
dissociation, gradient separation, and neurotrophic stuff (at least in
my experience). Do some searches for adult neuronal culture methods
and I think you'll see what I mean.
live neurons in your cell culture. We always work with either E16-17
or P1-3. For adult neuronal culture you need to do all kinds of
dissociation, gradient separation, and neurotrophic stuff (at least in
my experience). Do some searches for adult neuronal culture methods
and I think you'll see what I mean.
0 new messages