Description:
Molecular Biology and Genetics of Yeast (Moderated)
|
|
|
Mating efficiency for pretransformed library screening
|
| |
Hi zhenming:
I find the "Mating efficiency for pretransformed library screening" in
[link].
Now I have the same problem.
I do the yeast 2-hybrid screen by mating system from clontech. The
efficiency is very low. I use the Y2H gold and Y187. Do you have any advice
to me? Thank you.... more »
|
|
|
vector maps
|
| |
Dear bionetters,
Can anyone provide me the vector construct maps of
pSGP573 and pREP31X-lacZ
|
|
|
SMYTE31- Meeting-Sept 24-28-Antalya-Turkey
|
| |
Dear Colleques,
The 31st SMYTE (Small Meeting on Yeast Transport and Energetics) will be
held during September 24-28, 2013 at Porto Bello Hotel, Antalya, Turkey. Registration and abstract submission to the meeting is now open and the
deadline is June 15, 2013. Information about keynote speakers, meeting... more »
|
|
|
Save the Date for SERYM 2013
|
| |
Dear Colleagues and fellow yeast enthusiasts,
The 20th annual Southeastern regional yeast meeting (SERYM) will be held March 8-10, 2013 at the University of Alabama at Birmingham (UAB). For the 20th annual meeting our keynote speaker is Randy Schekman, a pioneer in membrane transport from yeast to humans.... more »
|
|
|
SGD Servers Moving to New Data Center
|
| |
Dear Colleague,
This is a message alerting you of planned downtime for the Saccharomyces Genome Database resources for the week of November 27, 2012.
Many SGD tools and resources will be unavailable from Tuesday afternoon, November 27th, until Thursday, November 29th because we will be moving our entire computing operation to a newly opened data center on Stanford campus. This new data center will provides a very robust environment including a more secure facility, a faster network, and a backup generator in the event of a power failure. There is also plenty of room for expansion. All of these features were missing from our current computing facility.... more »
|
|
|
S.cerevisiae GST expression vector
|
| |
Dear Yeast community,
We are looking for a S.cerevisiae expression vector that has a GST module, and also has a promoter and terminator, but not a carbon-source dependent promoter (eg. has a PGK or TEF promoter, rather than a GAL one). I haven't been able to find such a thing, but does anyone know if this exists and where we might be able to get it from?... more »
|
|
|
yeast cell cycle transcriptomes
|
| |
dear yeast folks,
this question is most likely trivial for you ... but i am an arabidopsis person, meaning that your help would be very much appreciated.
i am looking for transcriptome data of the yeast cell cycle. the minimum requirement would be a transcriptional series of g1-s-g2-m phases.... more »
|
|
|
antibody
|
| |
Hello Everybody,
I am looking for antibodies to check secreted recombinant proteins in
S.cerevisiae : BGL5 (beta-glucosidase from Pichia stipitis) and EGC1
(Endoglucanase from Pichia stipitis). If someone has them and can send me,
i would appreciate a lot.
Thanks in advance.
-- Burcin ALTUN... more »
|
|
|
PSY another name for W303?
|
| |
Dear Yeast Newsgroup,
Is W303 and PSY synonymous?
Thank you.
Sincerely,
John
-- John McLaughlin, PhD
Department of Plant Biology and Pathology (SEBS)
Rutgers University
Foran Hall, Room 212A
59 Dudley Road
New Brunswick, NJ 08901-8520
(Office) 848-932-6274
(Cell) 267-864-7129... more »
|
|
|
Help with IP and RNase A treatment
|
| |
Dear all I'm doing co-Immunoprecipitation experiments in yeast (s. cerevisae) and I think the interaction I see between my two favorite proteins is bridge by mRNA (because both are able to bind RNA). I'm looking for a protocol to perform RNAse treatment of my extracts before IP. First I plan to go with RNAse A but someone advices me to not used it because it could not degrade poly(A)tail protected by the poly(A) binding protein and mRNA in the closed loop conformation. I'm very surprised because RNase A seems to be communly used for this application.... more »
|
|
|
