Description:
Research on proteins and protein databases.
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Secondary Structure and Surface Accessibility predictions easily made
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Hi Everyone, I have seen that many people are interested in Secondary Structure predictions, and also interested in the surface of proteins. I therefore want to bring your attention to a paper I have recently published, which has the title: A generic method for assignment of reliability scores applied to solvent accessibility predictions.,... more »
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How to compare multiple pdb's according to secondary struc+torsion angles
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Dear All, I have approximately 40 to 50 pdb structures of homologous proteins that I want to compare. I am interested on plotting the sequence vs (secondary structure/torsion_angles/acces sibility/hydrophobicity). I have tried software like VMD and WHATIF to do such plots (or part of them) but I am getting problems when the number of structures compared is getting... more »
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plant protein extraction
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Dear all, I did protein extraction from fruit (Curculigo latifolia) according to the attached protocol. I got the white pellet. but while i left it to air dry briefly, i saw the color of pellet got darker, in some parts, it changed to black. i think while i took the phenol phase, unwanted substances entered.Do... more »
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Reactome Pathway Database User Survey
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Reactome is committed to providing access to high-quality pathway information and useful data analysis tools. With this in mind, we are actively soliciting comments from the research community in order to assess community needs. We are interested to hear about your experience with Reactome, and would like to know a bit about your... more »
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Practical Course in Biomolecular Modelling
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Dear colleagues, please be informed that online applications are still accepted for the following course until October 16, 2009: 8TH NCCR PRACTICAL COURSE IN BIOMOLECULAR MODELLING January 10 - 15, 2010 Kandersteg, Switzerland [link] Course topics include... more »
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SDS-PAGE
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Dear all, I perform *TCA-acetone, methanol washes and phenol precipitation* to extract protein from fruit sample. To see clear bands in SDS-PAGE, I need to remove polysaccharide. Does anybody know how to remove extracellular polysaccharide? Thanks in advance Eli
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Hettich centrifuge schematics
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Does anybody have a Hettich centrifuge schematics. The Hettich 30RF Universal centrifuge repair manual would be best but any Hettich should do as we just want to limit a broken centrifuge to a set speed. The expense to buy the circuit diagram is too much. So if anyone could help it would be greatly appreciated.... more »
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Proteins Digest, Vol 52, Issue 3
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Are you sure the aggregation is not caused by dna interweaving between protein molecules? If that's the cause, Benzonase will be a good choice for DNA clearance.
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Help in calculating the Tm and GC content of primer..
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Hi friends.. I am working with pET vector system. for expression of the enzme in E.coli BL21. I have to design primer with Nco1 site in forward primer. so can anybody will suggest me about the placing of ATG site of ORF...whether it should be in the primer's R.E site or else again i have to add in the primer aftre the R.E site.... more »
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QUERY
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Hi Monica, If your protein is in the pellets I hope you are using denaturing conditions to solubilize it before purification over Ni-NTA. solubilize the protein in urea or guanidine for one hour and spin down to get a clear lysis solution before you proceed to further purification steps Ni-NTa or gel.... more »
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