Description:
Requests for information and lab reagents.
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Why to use N-lauroylsarcosine for Hybridization?
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Did you get an answer?
I am asking the same question.
In the protocols I have found, it has been used as a prehybridization step, along with formamide. The molecule is pretty big, I suspect it is used to sandwich itself between the two strands of a DNA or RNA, while the formamide deionizes the the bases, stabilizing dehybridization, prior to Probe-hybridization, but I am guessing.... more »
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RNA/Protein UV crosslinking (UVXL) assay
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Dear all,
I am interested in an mRNA that has been described as being bound to the cytoskeleton. There are two published papers showing that a labelled fragment of the 3' UTR can be crosslinked to various (unidentified) proteins. I am hoping to identify the proteins and characterise them... more »
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PEDOFILO FIGLIO DI PUTTANA PAOLO BARRAI (MERCATO LIBERO), CHE SI VANTA DI AVER SODOMIZZATO I FIGLI COSTANZA BARRAI E RICCARDO BARRAI, DA QUANDO IN CULLA (E GIA' QUESTO LA DICE TUTTA) IMBOSCAVA (E IMBOSCA) € DI LEGA LADRONA CON ARRESTATO BELSITO.
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IN MOSTRUOSAMENTE CRIMINALE FINTER BANK LUGANO, CHIASSO, ZURICH E BAHAMAS, DI SCIACQUONE DI SOLDI MAFIOSI E, PARREBBE PURE SCIOCCANTEMENTE PEDOFILO GIOVANNI DEI CAS DA RANCATE IN NOVEZZANO ( [link] ), RICICLAVANO E RICICLANO OCEANI DI SOLDI ASSASSINI DI COSA NOSTRA, CAMORRA, NDRANGHETA, COME STRA RUBATI DA LL, LEGA LADRONA, E PDL, POPOLO DI LADRONI:... more »
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5x sds sample buffer become brown color while preparing
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Hello Everyone
I was trying to prepare 5x sds sample buffer and it has become brown color
instead of blue color. Could anyone please tell me what is the reason and
please tell me your suggestions.
The 5x sample buffer recipe
250mM tris-hcl ph 6.8(1M stock)------10ml
10% sds-------------------------4g r... more »
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Any experiences with Geneamp 9700 cycler?
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We've had the 9700 (over 10 years) for quite some time (over 10 years) with no real problems. Are you using the little black tube insert for single tubes and the red one for strip tubes? Without those you will crush the tubes (but I think these are similar to what gets used with the 2700 (which we also have). I will admit that I haven't watched the temps closely for quite some time, but we're not having problems with our PCR either.... more »
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RNA isolation from Formaldehyde fixed samples
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Dear All,
I have some cells fixed in 4% formalin (neutral I believe). I was wondering
if anyone has tried any RNA extraction on such cell/tissue samples, and if
you have any wisdom including kits, tricks, solutions, problems that you'd
share with me. I would appreciate it hugely.
Thank you much,... more »
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Retroviral vector packaging compatibility
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I have a lentiviral system working in my lab (pInducer), my PI wants
to know if we could use a pBabe based vector as well. pBabe
apparently has MMLV based LTR instead of HIV. My question is
whether the packaging constructs I have for lentiviruses will work
for MMLV?
I'm guessing that HIV and MMLV have different capsids, which would... more »
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protein estimation
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Hello all
I would like to quantify the protein concentration. I checked the nanodrop
manual in that they have given that I can estimate the protein by measuring
directly at 280 nm by giving extinction coefficient value without doing
bradford or lowry methods. I would like to choose measuring the protein... more »
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