Description:
Requests for information and lab reagents.
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Most cited research reagents and kits
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An interactive graph showing most cited research reagents and kits that are commercially available. The data is based on recent published journal articles, patents and patent applications. The current research reagent/kits listings are Transfection reagents, RT-PCR, Real-time PCR, RNA purification, cAMP assays, Cytotoxicity assays and Western blot reagents. Please visit: [link] Reagents&catId=1501 ... more »
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Purifying 5' phosphorylated primers for mutagenesis
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I'm doing some site-directed mutagenesis with a kit from NEB. It is recommended in the kit directions that the 5' phosphorylated primers used for the reaction are purified either using RP-HPLC or PAGE. My issue is that these purification methods are *really* expensive. (I've been getting my primers through Invitrogen, but I've also looked at a few... more »
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lyophilizing leaf tissue
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Hi savy methodologists, I need to lyophilize leaf tissue prior to shipping overseas. The tissue will be used for analysis of lipids, which are vulnerable to endogenous lipases, so I am planning to collect the tissue into liquid N2, freeze dry and ship on dry ice to try to ensure that these lipases don't have a chance to do... more »
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qPCR 2010 Event - Call for Abstracts
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qPCR 2010 Event - Call for Abstracts [link] ------------------------------ ----------------------- BioEPS GmbH is organizing the qPCR 2010 Event taking place April 7th – 9th, 2010 in Vienna, Austria. Scientists from all around the world will come to exchange ideas, share experiences, and discuss the... more »
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Sequential restriction digest
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Dear all, I want to do a SacI/SalI digest of my plasmid in order to subclone my insert (1.2 kb) into another vector. According to NEB, the REs need different buffers, so I would have to do a sequential digest. Does anyone have a working protocol for sequential digests? Particularly I would like to know how to proceed once the first digest i.e. with SacI... more »
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RNA ligation
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Hi everyone, I hoping to ligate a synthesised oligonucleotide to the 5' end (uncapped but not dephosphorylated) of an mRNA population. Can anybody tell me the best way of doing this? many thanks Chris
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AKTA FPLC Mixer 925 Problem and Solution
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This is not exactly a method but could save you money. We have an AKTA FPLC with the Mixer 925 unit. We don't have a service contract so when the mixer unit stopped working and the system provided a short-circuit warning message, we asked the price of a new one. We were quoted £10,000, which is half the price of the whole... more »
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a queston
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Dear colleagues I appreciate if anybody can help me in the following problem: I used Lpp'-ompA system (developed by Georgio et al., 1996) for surface display of a bacterial metallothioneine fused to chitin binding domain and 6Xhis-tag (on the surface of E.coli BL21). The length of this passenger protein is approximately 15kDa. We did metal binding assay using whole cell and we measured adsorption using a solution of cadmium Cd(NO3)2 in Tris-Hcl pH7.0. But it didn't show any adsorption. Hydropathy plot showed the passenger proteins are hydrophile and also it didn't show any transmembrane domain. I would like to know why does not this protein show any adsorption?... more »
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