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Newsgroups: bionet.molbio.methds-reagnts
From: "Jayakumar, R" <R.Jayaku...@roswellpark.org>
Date: Tue, 7 Aug 2012 18:37:23 +0000
Local: Tues, Aug 7 2012 2:37 pm
Subject: RE: Overgrown (3day) LB plates ok to use?
If they were antibiotic resistance plates, they are probably useless and full of satellite colonies.
Jay -----Original Message-----
From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of David Liu Sent: Monday, August 06, 2012 7:39 PM To: meth...@magpie.bio.indiana.edu Subject: Overgrown (3day) LB plates ok to use? I left a replica LB Amp plate in the 37C over the weekend, and I'd like to know if they're still good to use. I've started liquid cultures but if they grow, can I use the minipreps without resequencing the whole plasmid? It's a replica plate, so I don't necessarily need single colonies, and satellite colonies are not an issue. I am primarily concerned about spontaneous mutations that may have accumulated. Any ideas? _______________________________________________
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