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SDS PAGE
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Nadim
Dec 2, 2001, 10:49:02 PM12/2/01
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Hi
I have been having problems with running my SDS PAGE. Ive bee running an 8%
running with a 4% stacking yet recently for some baffling reason my marker
has not been seperating correctly. I am only seeing the top two (heaviest
two) bands appear immediately once the samples get to the running part. Then
only the heaviest two bands (120, and 90KD) are migrating throughout the
gel, it is almost as if the remaining bands of the marker are 'too small'
for the gel and dont appear! Ive made new Tris buffers and even though Im
using the same acrylamide others in the lab have used it with no problems.
Ive also made fresh running buffer. I checked my recipes and they are
correct, yet i still see the smae phenomenon. I am baffled.
I have been having problems with running my SDS PAGE. Ive bee running an 8%
running with a 4% stacking yet recently for some baffling reason my marker
has not been seperating correctly. I am only seeing the top two (heaviest
two) bands appear immediately once the samples get to the running part. Then
only the heaviest two bands (120, and 90KD) are migrating throughout the
gel, it is almost as if the remaining bands of the marker are 'too small'
for the gel and dont appear! Ive made new Tris buffers and even though Im
using the same acrylamide others in the lab have used it with no problems.
Ive also made fresh running buffer. I checked my recipes and they are
correct, yet i still see the smae phenomenon. I am baffled.
Tyson
Dec 3, 2001, 2:39:08 PM12/3/01
to
what about your samples? are they running ok? Is your BPB marker running
ok? If so than your protein marker must be the problem...try some other
markers or get a new one.
ok? If so than your protein marker must be the problem...try some other
markers or get a new one.
"Nadim" <nm...@columbia.edu> wrote in message
news:2001120303490...@ww02.jatek.com...
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