Homemade FTA paper and kit???

[Peter Willingmann]

hello netters;
can anybody point me to a paper where the basis of FTA techniques from
Whatman is described in some more detail??
I would like to have a better understanding how this work and perhaps
to make a homemade kit.
thanks in advance
ciao
peter

[Peter Willingmann]

DK wrote:
thanks a lot... how stupid of me not to come to the same conclusion..

ciao
peter

>
> Nothing fancy there. It's just a thick filter paper with dried Tris,
> EDTA and SDS. You spot cell onto it, SDS lyses them, then
> the whole thing is dried. Quick wash with TE removes some
> DNA and most of the SDS/EDTA, then more DNA is eluted
> and used for transformation (when storing clones) or PCR.
> For cleaner things, phenol and/or isopropanol washes
> can be added before elution.
>
> It is described in the multitude of patents by inventor
> Leigh A. Burgoyne and Assignees either Flinders Technologies
> or Whatman. E.g., # 5496562:
>
> ~ 50 ul of 2% SDS, 10 mM EDTA, 60 mM tris spotted on
> 1 cm2 of Whatman 3MM and dried.
>
> FTA kits have some other filter paper, thicker than 3MM but
> it really does not matter. Also, the concentrations seem to
> be an overkill. Long ago, I soaked some 3 MM in just
> TE + 1% SDS, dried and spotted 5 ul of several overnight
> cultures. A year after, storing the paper in the drawer, all
> clones gave lots of transformants after electroporation.
>
> This is a good candidate for inclusion into lab generics Wiki
> found at http://methodsandreagents.pbwiki.com/
>
> DK

[vive...@gmail.com]

Great thread.

The patent mentions adding Uric Acid for ling term storage

<a href="http://www.google.com/patents?
id=w_UiAAAAEBAJ&zoom=4&dq=5496562&pg=PA4&ci=98,763,396,282&source=bookclip"><img
src="http://www.google.com/patents?
id=w_UiAAAAEBAJ&pg=PA4&img=1&zoom=4&hl=en&sig=ACfU3U0WYtwz0NPYQmf7XsyPDlZ8932rzA&ci=98,763,396,282&edge=1"
border="0" alt="tetracetic acid and iii an anionic detergent such as
sulphate and optionally iv uric acid or a urate salt By way of example
a particularly according to this aspect of the absorbent cellulose
based paper such a minimal loading per sq cm of a EDTA 0.5 micromols
146.1 mg b Tris 8 micromols 968.8 mg of c SDS 1 mg and optionally "/></
a><br/><a href="http://www.google.com/patents?
id=w_UiAAAAEBAJ&zoom=4&dq=5496562&pg=PA4&ci=98,763,396,282&source=bookclip">Solid
medium and method for DNA storage Leigh A. Burgoyne</a>

VK

On Jul 17, 8:13 am, d...@no.email.thankstospam.net (DK) wrote:

> In article <6e8pa2F5etb...@mid.dfncis.de>, Peter Willingmann <bt8a...@uni-hamburg.de> wrote:
> >hello netters;
> >can anybody point me to a paper where the basis of FTA techniques from
> >Whatman is described in some more detail??
> >I would like to have a better understanding how this work and perhaps
> >to make a homemade kit.
>