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Why to use N-lauroylsarcosine for Hybridization?
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michel saremi
May 2, 2013, 11:46:18 AM5/2/13
to met...@magpie.bio.indiana.edu
Did you get an answer?
I am asking the same question.
In the protocols I have found, it has been used as a prehybridization step, along with formamide.
The molecule is pretty big, I suspect it is used to sandwich itself between the two strands of a DNA or RNA, while the formamide deionizes the the bases, stabilizing dehybridization, prior to Probe-hybridization, but I am guessing.
I look forward to hear your thoughts on the matter.
Best Regards
Michel Saremi
I am asking the same question.
In the protocols I have found, it has been used as a prehybridization step, along with formamide.
The molecule is pretty big, I suspect it is used to sandwich itself between the two strands of a DNA or RNA, while the formamide deionizes the the bases, stabilizing dehybridization, prior to Probe-hybridization, but I am guessing.
I look forward to hear your thoughts on the matter.
Best Regards
Michel Saremi
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