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Re: Loading dye

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Barbara MacGregor

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Oct 26, 2009, 1:12:37 PM10/26/09
to met...@oat.bio.indiana.edu
Xylene cyanol definitely has a different color, though, at least as
I've used it - blue-green rather than blue.

But those are the only blue dyes I can think of, so that's probably
it...

Barbara

>
> Raymond Dalgleish <rw...@NOSPAMle.ac.uk> wrote:
>
>> Any ideas about what the blue dye might have been in this incorrect
>> loading dye?
>
> This sounds very much like Xylene cyanol. Our loading biuffer contains
> both dyes.
>
> Christian
>

Jayakumar, R

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Oct 26, 2009, 2:05:08 PM10/26/09
to Barbara MacGregor, met...@oat.bio.indiana.edu
Bromo phenol blue.. Frequently added with xylene cyanol in DNA loading dyes.

Jay

Barbara

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Christian Praetorius

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Oct 27, 2009, 5:30:20 AM10/27/09
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Barbara MacGregor <bmac...@unc.edu> wrote:

>Xylene cyanol definitely has a different color, though, at least as
>I've used it - blue-green rather than blue.

Thats right. But it migrates around 10k in a 1% gel.

Christian

--
X-no-Sig: yes

Jayakumar, R

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Oct 27, 2009, 5:16:56 PM10/27/09
to Christian Praetorius, met...@magpie.bio.indiana.edu

Hmm.. XC I think moves with the 4K band and above the blue dye front of BPB (the blue dye he is talking about) moves with a 300 bp band on a 1% gel
Jay

Barbara MacGregor <bmac...@unc.edu> wrote:

Christian

--
X-no-Sig: yes

Raymond Dalgleish

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Oct 28, 2009, 3:49:11 AM10/28/09
to
The issue is now solved. We ran a 1% agarose gel of the suspect loading
dye alongside size markers and other loading dyes of known provenance.

The dye which had been described as being bromophenol blue was in fact
very highly concentrated xylene cyanol, so it looked dark blue rather
than blue/green.

The person responsible will be re-educated.

Thanks for all the replies.

Raymond

WS

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Oct 28, 2009, 12:04:00 PM10/28/09
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> The person responsible will be re-educated.

Means hopefully: Will be sentenced to bake Black-Forest-Cake (under
surveillance) for the next lab meeting?

Cheers, Wo

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