Google Groups no longer supports new Usenet posts or subscriptions. Historical content remains viewable.
Dismiss
how to find Kd
1 view
Skip to first unread message
ruchi jain
Dec 18, 2009, 2:14:41 PM12/18/09
to met...@magpie.bio.indiana.edu
Hello,
I am trying to find the kd for my protein which binds GTP. I have done two
sets of experiments:
1) taken constant protein and increased unlabeled GTP and monitored the
tryptophan fluorescence quenching.
2) constant labeled (mant)-GTP and increased the protein and monitored the
fluorescence increase with increasing protein.
I am trying to find the kd for my protein which binds GTP. I have done two
sets of experiments:
1) taken constant protein and increased unlabeled GTP and monitored the
tryptophan fluorescence quenching.
2) constant labeled (mant)-GTP and increased the protein and monitored the
fluorescence increase with increasing protein.
I want to know if someone can help me and tell the equations (and the
formulas) to feed these values in , in order to calculate the kd.
Thanks
Ruchi
0 new messages