SDS that won't re-dissolve after autoclaving

[Duncan Clark]

Hi Folks,

Anyone any idea why if one autoclaves 10% (w/v) SDS prepared in MilliQ
water for 25mins instead of 15mins, it will no longer go back into
solution even when diluted 100fold into warm water? Biorad is the source
a. If you know how to get it back into solution, please tell.

I've never come across this before. Most odd.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

[Joshua Silverstein]

It could be hydrolysis of SDS occurring during autoclaving, producing
sulfuric acid and decanol. If you can use a buffer, I highly suggest that..
The reaction that occurs is further catalyzed by the product, thus it only
gets faster and faster. Hope this helps.

Josh

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[Dr. Hiranya S. Roychowdhury]

Hydrolysis by autoclaving SDS I haven't heard of, but it is a common
occurrence that SDS precipitates following autoclaving. I would look at
the water you are using. The K-salt is insoluble and autoclave
environment may be introducing some potassium salt. Is it stll insoluble
after you let it sit at room temp?

> It could be hydrolysis of SDS occurring during autoclaving, producing
> sulfuric acid and decanol. If you can use a buffer, I highly suggest
> that..
> The reaction that occurs is further catalyzed by the product, thus it only
> gets faster and faster. Hope this helps.
>
> Josh
>
> On Fri, Dec 18, 2009 at 12:06 PM, Duncan Clark
> <blac...@abuse.plus.com>wrote:
>

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>> Methods mailing list
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> http://www.bio.net/biomail/listinfo/methods
>

--
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003

[Duncan Clark]

Historians believe that in newspost
<mailman.1.1261168...@net.bio.net> on Fri, 18 Dec 2009,
Joshua Silverstein <silverste...@gmail.com> penned the following
literary masterpiece:

>It could be hydrolysis of SDS occurring during autoclaving, producing
>sulfuric acid and decanol.

Looks like that is the problem.

>If you can use a buffer, I highly suggest that..

Can't really.

>The reaction that occurs is further catalyzed by the product, thus it only
>gets faster and faster. Hope this helps.

Must be the particular batch of Biorad SDS is very sensitive to this as
other supplies that we have seem fine.

Thanks to you and DK for your help.