Description:
Research on microbial biofilms (Moderated)
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Query - Nucleic acid extraction from biofilms
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Hi, 1. What is the most appropriate method for growing biofilms if the objective is to obtain atleast 2ug of DNA or RNA ? 2. I choose to grow them in static mode over a glass slide 1" x 3" immersed in a culture broth, will this method work? 3. If this be the method after how many hours of incubation will i be able... more »
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CRA troubleshooting
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Dear all, Many of the questions on this list relate to Congo Red Agar, so I am hopeful that someone will relate to my experience with it. I plan on using CRA to demonstrate biofilm formation in staph grown under different conditions on agar. As mentioned in Freeman and other sources, colonies should be... more »
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confocal microscopy related query
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Dear Sir i am working on a project on quorum sensing and quenching of pathogenic microbes. Please help me with the protocol that i should follow to take a confocal scan of the biofilm. so far i have checked the effect of a plant exact on the biofilm formation of pseudomonas. the treatment showed reduction in biofilm when quantified... more »
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biofilm question
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Hello, I am a Biofilms mailing list membership, I want to know the Agar red Congo composition , I want to study the biofilm phenotypic expression in Satphylococcus aureus. Best regards Lic. Joan Peña Rodríguez Microbiology Laboratory, CENLAC National Center for Animal and Plant Health (CENSA) Po Box 10, San José de las Lajas, CP 32700... more »
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Request for information
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Hi All, I am new member to this forum. Let me introduce myself. I am Yogesh Joshi from University of Salerno,Italy. Currently I am doing research on single species biofilm (Kluyveromyces marxianus) and its application in fermentation. Anyone working on this subject or can anyone provide the email ids for the people who are working.... more »
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Hello
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Prem Saran mailing .. Is there a standard procedure for Growth of Biofilms Invitro conditions ?? Also on Biofilm assays . Thanx
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Removal of biofilms from tubes
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Dear all, I'm looking for the best way to safely remove biofilms from tubes. The tubes are made of PE (poly-urethane). I've seen several reports on sonication for the removal, but in those reports the material is always steel or iron. I'm wondering if the sonication procedure should change if the material is different.... more »
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pseudomonas aeruginosa biofilm formation in lab
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helo frnz, first of all, i wud lik to thank d moderator of ds grp fr havin a discussion forum on Biofilms.. I am new to this group.. i ve jus subscribed.. i am studyin in chennai.. intrstd to do proj in pseudomonas aeruginosa biofilms.. i ve onl basic knowledge abt wat biofilm is.. so pls help me in carryin out this proj.. i wanted to knw as how p.aeruginosa can... more »
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