Description:
Discussion from Biotechniques. (Moderated)
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dear bionet.journals.letters.biotec hniques readers
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-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 It's getting a bit tiresome seeing one misguided American after another with their cutsie little yellow or red-white-blue ribbons on their outsized SUVs. Yeah, I guess it's the thing to do; maybe part of that whole soccer-mom culture. Unfortunately, the only thing they demonstrate is that the person... more »
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Here it is. The smoking gun.
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The memo that has "IMPEACH HIM" written all over it. The top-level government memo marked "SECRET AND STRICTLY PERSONAL", dated eight months before Bush sent us into Iraq, following a closed meeting with the President, reads, "Military action was now seen as inevitable. Bush wanted to remove Saddam through military action... more »
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Last rites declaration of Ioannes Paulus PP. II (Karol Wojtyla)
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"The unforgiveable sins this earth must confront and overcome are Nationalism, capitalism, and hoarding. The idea of every nation should be forgot, price should be struck from the commons, and princes should be seen for the devils they are. The sins include our church, secret societies, and other religions which make of... more »
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plain simple cheap fair
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Usha, rethink the Cool + the Shoe phil knight had a dream. he'd sell shoes. he'd sell dreams. he'd get rich. he'd use sweatshops if he had to. then along came a new shoe. plain. simple. cheap. fair. designed for only one thing: kicking phil's ass. the unswoosher $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$ $$$$$$$$$$$$$$$$$$$$$$$$$$$$$$ $$$$$$$$$$... more »
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Newsgroup Discontinued
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As previously posted, the BioTechniques newsgroup has been discontinued as of February 1, 1999. Please visit our web site at [link]. BioTechniques Editorial Office Eaton Publishing 154 E Central St Natick, MA 01760 USA Tel 508-655-8282 Fax 508-655-4356 bioedi...@BioTechniques.com... more »
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Newsgroup Discontinued
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The BioTechniques newsgroup will be discontinued on February 1, 1999. Please visit our website at [link] BioTechniques Editorial Office Eaton Publishing Co 154 E Central St Natick, MA 01760 USA Tel 508-655-8282 Fax 508-655-4356 bioedi...@biotechniques.com
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in vitro translation problems
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Would like to know info abt in vitro translation of memebrane bound proteins, eg human muscarinic receptor.. have problems with translation, protein incorporates radioactivity, but does not move into gel.. despite several attempts. is it due to agregation of the protein molecules since there is no membrane present??... more »
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PCR of GC-rich sequence
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Dear Biotechniques, I am currently working on constructing a clone but have ran into problems as most graduate students do. The region I am seeking to amplify is GC rich and the plasmid DNA could be forming tertiary complexes preventing PCR amplification. I have consulted the texts and tried increasing MgCl2 concentration as well... more »
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