Description:
Research and support on automated DNA sequencing (Moderated)
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Course: Next-Generation Sequencing as a Diagnostics Platform
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**Course Announcement**
Next-Generation Sequencing as a Diagnostics Platform
June 4, 2012
Loews Hotel, Philadelphia, PA
The focus of this short course will be on understanding the use of NGS in
clinical diagnosis, practical implementation of NGS in clinical laboratories and analysis of large data sets by using... more »
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Library prep inquiry
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Hi,
I have a question related to library prep of genomic DNA (*C.elegans*)
prior to whole genome sequencing using Illumina platform.
With previous samples, my lab had used fragmented DNA of size range 100-250
bp before sending it for sequencing. However, the Bioanalyzer results gave
us an unwanted DNA smear at 90 bp region which we presumed were... more »
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ABI 3730xl Sequencing
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Good Day, My name is Nobby Clark, owner of Carmet Scientific. I deal with pre-owned and surplus biotech and lab instrumentation. I am interested in purchasing ABI 3730XL's or ABI 3130XL's. If you have or know of anyone who has these units available, could you please refer them to me? Other items... more »
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PDF request
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Dear all, I'm trying to find the following paper: LM Smith, RL Brumley, Jr, EC Buxton, M Giddings, M Marchbanks, X Tong. Highspeed automated DNA sequencing in ultrathin slab gels. Method Enzymol 271:219–237, 1996 If anyone can send me a pdf, or point me to the right direction, I'd be grateful. Thank you very much.... more »
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Searching for LICOR4300
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Hi, we are currently seeking for a automated sequencer LICOR 4300 Please reply to this message if you have this machine for sale. We have to replace one of our units which definitely broken Best regards, Marco Maccaferri
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3730xl Sequencer
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Hello,My name is Nobby Clark, owner of Carmet Scientific. I deal with pre-owned and surplus biotech and lab instrumentation. I am interested in purchasing ABI 3730XL's or ABI 3130XL's. If you have or know of anyone who may have these units available, could you please... more »
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Reusing Centri-sep columns
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Hi, I saw your posting online about reusing Centri-sep columns. I was wondering how you much gel you use? Do you actually weigh out a specific mass? I've been looking online for the mass that Princeton uses but I cannot find it. Thank you! Kris Bickley Seattle Pacific University
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Genotyper and Windows 2000
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Dear Lynn Petukhova, I have just see your question about Genotyper software and Windows XP in a web forum. Could you say me how could you run Genotyper in Windows Xp system, please? Thank in advance, Miguel Angel Dr. Miguel Angel González Pérez Departamento de Biología Universidad de Las Palmas de Gran Canaria... more »
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quality scores using consed/phrap/phred
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Let me open with the standard disclaimor that I'm new to these packages and therefore apologize if i'm submitting an obvious question. I have received a set off .SFF files that I would like to process/align. Running consed gives me an .ace file with fragments and their starting points in the reference sequence. This is great.... more »
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