Description:
Information about the Arabidopsis project (Moderated)
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Deadline Approaching - CFP - APBC2010
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============================== ============================== === Deadline Approaching 20 July ! - Call for Papers - APBC2010 The Eighth Asia-Pacific Bioinformatics Conference (APBC2010) Bangalore, India, 18-21 January 2010 [link] ============================== ============================== ===... more »
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Job Opening: Plant Ontology Project Coordinator
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Deadline extended until July 31st. Also, if you are attending the following meetings please feel free to contact us to learn more about this position and the project: ASPB-Plant Biology 2009 : Contact Pankaj Jaiswal BSA Botany & Mycology 2009: Contact Aaron Liston Position Title: Research Associate (Senior Postdoc) / Research Associate (Postdoc)... more »
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Reply to "Could anyone help me on Arabidopsis genomic DNA" (Arab-gen Digest, Vol 50, Issue 15)
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Hi Chunmei, I have often had problems with PCR using genomic DNA as a template. For the most part, using the CTAB DNA preparation versus other faster, yet dirtier, preparations has helped tremendously. Since you are already using this preparation method, you might now want to try using additives that will help melt your DNA/primers, stabilize the polymerase, etc (for example add DMSO, glycerol, glycine betaine or BSA). BSA has helped more than any other additive for me. Also, increasing the buffer (complete with Mg2+) concentration can also help (up to 1.6 X final). Finally, have you tried varying the annealing temperature for your primer set? I suggest using a gradient machine to test a rangeof tmperatures. Please keep in mind that all PCR products are unique in their needs, and that the best conditions for your reaction must be determined empirically. Here is a link to a website that helped guide me toward possible PCR variations to try: [link].... more »
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Arab-gen Digest, Vol 50, Issue 15
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Dear Chuanmei Zhu DBBS(plant biology), Washington University, St.Louis, MO,USA. 63130 the genomic DNA of arabidopsis has the introns,UTRS,etc thats why you may not be able to amplify the expected amplicon, so its better to amplify from CDNA, if you have plant tissue prepare RNA , from that prepare CDNA using... more »
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Twp postdoctoral positions at the University of North Texas
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Two postdoctoral positions are available in the Department of Biological Sciences at the University of North Texas, which is located in Denton near Dallas (Texas). Position 1 (Requisition #090681): Postdoctoral position in Plant –Insect Interaction University of North Texas is seeking a full time postdoctoral research associate to study plant defense response. The research emphasis will be on studying Arabidopsis defense responses to the green peach aphid. We seek candidates with a Ph.D. in molecular biology, entomology or a related field. Applicants should have expertise in plant molecular biology. Candidates with expertise working with aphids are preferred. The successful candidate must be able to design and conduct independent experiments. Excellent oral and written communication skills and the ability to work well in a team-based/collaborative research atmosphere are essential. The Postdoctoral Research Associate will work under the direction of Dr. Jyoti Shah in the Department of Biological Sciences.... more »
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Could anyone help me on Arabidopsis genomic DNA cloning?
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Hi, I am a graduate student in Washington University. I have trouble in cloning a ~1.9 kb DNA fragment from Arabidopsis genomic DNA recently. The genomic DNA was prepared by CTAB method and was dissolved in H2O. For 25ul PCR reaction, I use DNA ~500ng, MgCl2 1.5mM, dNTP 160uM, Taq 2.5U, primer each... more »
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M.Sc. Position available: ABC transporters in plant secondary metabolism
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M.Sc. Position available: ABC transporters in plant secondary metabolism. A graduate student is sought for a(n) MSc/PhD project under the joint supervision of David Bird and Peter Facchini in the Department of Biological Sciences at the University of Calgary. The goal of this research program is to identify and characterize ABC transporters involved... more »
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Embryogenic culture
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Dear Sir/ madam I would like to post my query in this page . Thank you . Puna "Query" Dear arab-gen users, I am posting a query related to the problem of contamination in embryogenic cultures. If anybody has idea to overcome it, I highly appreciate any kind of your comments. I followed the method described in the paper by Mordhorst et al., 1998. As mentioned in the Mordhorst et al., 1998, seeds were... more »
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dexamethasone and microarrays
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Hi there Can anyone point me to Arabidopsis microarray experiments where the effect of dexamethasone on gene expression in *control* lines (preferably in roots) has been reported? This would have been done for comparison with transgenic lines expressing a Dex-inducible gene. Many thanks Brian... more »
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protein extraction from cambium
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Hello, Here is a question from one of my students. It's not about Arabidopsis, but I would imagine that some readers of thi list have ideas about extracting from very low protein concentration? QUOTE: I am in Finland right now working as a trainee for 4 months (well 2 months left to go). My project is looking at telomerase activity in... more »
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