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Description: Information about the Arabidopsis project (Moderated)
 

Arabidopsis in teaching intro. biology 
  Dear Colleagues, I would like to know if any of you use Arabidopsis in introductory biology teaching courses? If so, would you be so kind as to share with me the experiment that you perform, the strains/mutants used, and a general idea in how long the experiment takes, and the source and cost of materials?... more »
By Eric D Brenner  - Nov 5 - 1 new of 1 message    

looking for pBI221 
  Dear colleagues, I am looking for an old plasmid, pBI221 (if I recall well, a pUC-based plasmid with a 35Spromoter-GUS-Nosterm construct in it). It used to be sold by Clontech, in the old days.If anyone has some left and could spare me an aliquot, I would be grateful. Regards Jean-luc
By Gallois  - Nov 4 - 1 new of 1 message    

Assistant Professor Position Available; University of Missouri 
  UNIVERSITY of MISSOURI Position Description: The Division of Plant Sciences, University of Missouri, invites applications for a tenure-track position in plant cell wall biology. The 12-month, assistant professor position is 60% research and 40% teaching. Responsibilities: The successful candidate will be expected to... more »
By Stacey, Gary  - Nov 3 - 1 new of 1 message    

Regarding your question on Agrobacterium transformation 
  Hi Ben, I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed.... more »
By shahzad anjam  - Nov 3 - 1 new of 1 message    

The timespan between Agrobacterium infection and active exgenous gene expression 
  I am wondering, after infected by Agrobacterium tumefaciens, how soon will the gene in the T-DNA fragment will start to be actively expressed in Arabidopsis. Are there any studies on this topic? Thanks. Ben
By Peng Wang  - Nov 1 - 1 new of 1 message    

LR Gateway reaction pENTR223.1-Sfi-ORF in to pK7WGF2- problem 
  My question is did any of you had a problem doing the LR reaction (gateway recombination) with the* *pENTR223.1 Sfi-ORF (from ABRC) and the* *pK7WGF2 (or any other Gateway Destination vector). If you had this problem how did you solve it? Thanks
By Yuval Kaye  - Oct 26 - 1 new of 1 message    

postdoc position: Arabidopsis-aphid interactions 
  A postdoctoral position is available to study interactions between Arabidopsis and Myzus persicae (green peach aphid) in Georg Jander’s lab at the Boyce Thompson Institute. The focus of this research is the functional analysis of aphid saliva, with particular emphasis placed on identifying salivary effectors that either facilitate insect feeding or... more »
By Georg Jander  - Oct 27 - 1 new of 1 message    

Job posting 
  To whom it corresponds, Find below the description of our job offer with kindly request to post it in the Arabidopsis Bionet: International PhD Program Tuebingen, Spring 2010 The Max Planck Institute for Developmental Biology and the Friedrich Miescher Laboratory in Tuebingen, Germany, invite applications for PhD positions in: Evolutionary Biology, Ecological Genomics, Developmental Genetics, Animal Development , Plant Development, Quantitative Genetics, Molecular & Cell Biology, Biochemistry, Structural Biology and Bioinformatics... more »
By Carolina Mueller  - Oct 27 - 1 new of 1 message    

Arabidopsis cDNA expression library for yeast needed 
  Hi all, We are looking for Arabidopsis cDNA expression library for yeast driven by GAL promoter (or any other inducible promoter), preferably one enriched with low abundant cDNAs . If anyone knows where I can find it or if you have one, please drop me a line. Any help will be appreciated. Thank you, udi... more »
By Ehud Katz  - Oct 23 - 1 new of 1 message    

fluorescent protein vectors 
  Hi, I'm looking for a pDONR P2R-P3 vector or a binary vector without promoter containing a C-terminal fluorescent tag other than YFP or GFP. Thanks Nuria
By Nuria Sanchez Coll  - Oct 24 - 1 new of 1 message    

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