questions with Wisc line primer

[Smith Chiang]

Hello At colleagues,



I am going to use a few Wisc lines ordered from ABRC for Arabidopsis genes.
could you please tell me the vector primer sequence so that I can use to
screen out homozygous lines usng PCR? With Iselectprimers in T-DNA Express
website, I can only get the LP and RP primers and donot know the
vector-specific primer for PCR screening.





thanks,


Smith

[Tom Leustek]

We have used a primer called p745 (5'-AACGTCCGCAATGTGTTATTAAGTTGTC-3')

I don't have the reference handy.


Thomas Leustek, Ph.D.
Professor
Department of Plant Biology and Pathology
Rutgers-The State University of New Jersey
School of Environmental and Biological Sciences
59 Dudley Road
New Brunswick, New Jersey 08901-8520
Tel: 1(848) 932-6296
Fax:1(732) 932-0312
Cell:1(908) 451-3266

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[Cesar Barragan]

Hello There,

In regards of T-DNA Wisconsin lines vector sequence and primers please refer to the links below.
https://mywebspace.wisc.edu/groups/Krysan/Web/2010/default.htm
http://www.plantphysiol.org/content/137/1/3.full

Cesar Barragan
The Salk Institute for Biological Studies
Genomic Analysis Laboratory
10010 North Torrey Pines Road
La Jolla, CA 92037
Tel 858-453-4100 ext 1108
barr...@salk.edu