[Arabidopsis] request for info on T-DNA orientation

[Abo-Ogiala, Atef]

Please I need answer for the same question:

I want to find the orientation of T-DNA insertion in some mutants of
Arabidopsis.

Best regards

Atef Abo-Ogiala

PhD student

Büsgen-Institut

Forstbotanik und Baumphysiologie

Georg-August Universität

Büsgenweg 2

37077 Goettingen

Germany

Tel: +49 (0)551 - 39 9362

Fax: +49 (0)551 - 39 22705

[Adrian]

On Jun 15, 7:40�am, "Abo-Ogiala, Atef" <Atef.Abo-Ogi...@forst.uni-

goettingen.de> wrote:
> Please I need answer for the same question:
>
> I want to find the orientation of T-DNA insertion in some mutants of
> Arabidopsis.
>
> Best regards
>
> Atef Abo-Ogiala
>
> PhD student
>

> B�sgen-Institut
>
> Forstbotanik und Baumphysiologie
>
> Georg-August Universit�t
>
> B�sgenweg 2

>
> 37077 Goettingen
>
> Germany
>
> Tel: �+49 (0)551 - 39 9362
>
> Fax: +49 (0)551 - 39 22705

Depending from which mutant bank you request the mutants is possible
to see the orientation of the insertion on the SIGnAL T-DNA Express
tool (http://signal.salk.edu/cgi-bin/tdnaexpress), just need the AGI
number of the interrupted gene and the code of the mutant. The page
will display the gene orientation on the genome and the orientation of
all T-DNA insertion mapped to that gene. If you have your own mutant
bank generated by T-DNA insertion, to find the T-DNA orientation you
should perform a TAIL-PCR using the T-DNA that you prefer.

I hope this help...