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LR Gateway reaction pENTR223.1-Sfi-ORF in to pK7WGF2- problem
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Yuval Kaye
Oct 26, 2009, 5:44:06 AM10/26/09
to Arab...@magpie.bio.indiana.edu
My question is did any of you had a problem doing the LR reaction (gateway
recombination) with the* *pENTR223.1 Sfi-ORF (from ABRC) and the* *pK7WGF2
(or any other Gateway Destination vector). If you had this problem how did
you solve it?
Thanks
--
Yuval Kaye
Department of Plant & environmental Sciences
Institute of Life Sciences
The Hebrew University of Jerusalem
Givat Ram, Jerusalem, 91904, Israel
E-Mail:yuval...@mail.huji.ac.il <E-Mail%3Ayuva...@mail.huji.ac.il>
recombination) with the* *pENTR223.1 Sfi-ORF (from ABRC) and the* *pK7WGF2
(or any other Gateway Destination vector). If you had this problem how did
you solve it?
Thanks
--
Yuval Kaye
Department of Plant & environmental Sciences
Institute of Life Sciences
The Hebrew University of Jerusalem
Givat Ram, Jerusalem, 91904, Israel
E-Mail:yuval...@mail.huji.ac.il <E-Mail%3Ayuva...@mail.huji.ac.il>
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