Description:
Research on Chlamydomonas and other green algae (Moderated)
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Postdoc: ArizonaU.Multicellularity
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Postdoctoral Position: Steps to multicellularity in volvocine green algae
A postdoctoral position is available in the laboratory of Dr. Richard E.
Michod ([link]) in the Department of Ecology
and Evolutionary Biology at the University of Arizona (Tucson, AZ;
[link]). We are looking for a motivated, enthusiastic... more »
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Fluorescence-labeling of zygotes
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Does anyone know specific techniques/markers/proteins for
fluorescently labeling and distinguishing vegetative cells from
zygotes for C. reinhardttii? I would like to be able to quantify the
two from a liquid culture.
Thanks,
Hanna Koch
PhD Student
Max Planck Institute for Evolutionary Biology... more »
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Motility difference between CC-1009 & CC-1010?
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I am using strains CC- wt 1009(-) & 1010(+) for a sexual reproduction
experiment and have noticed a two-fold difference in growth and
motility between the 2 strains; with 1010(+) growing faster and having
a much higher proportion of motile individuals.
The strains have been treated with identical conditions, were plated... more »
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Crude Thylakoid Prep
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Does anyone have a protocol you recommend for high through-put, crude
thylakoid preps? Preferably something an undergraduate could do in a few
hours?
Thanks in advance for any suggestions!
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Volvocine algae technician position in Tucson
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A Laboratory Technician position is available in the laboratory of Dr. Richard E. Michod ([link]) in the Department of Ecology and Evolutionary Biology at the University of Arizona, Tucson ([link]<[link]>.arizona.edu<[link]>). We are looking for a motivated, enthusiastic and independent individual with a biological science background (molecular / cell biology is preferable). Responsibilities will include:... more »
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problem preparing Hutners trace elements...
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Hi Adriana,
We have always prepared the Hutner's trace element solution according to the described recipe. An important point was to keep the temperature to 70 degrees C or higher when adjusting the pH to 6.5-6.8 with KOH. We never used bubbling air to accelerate the formation of the precipitate and we stored... more »
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Fw: [Chlamydomonas] problem preparing Hutners trace elements...
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I prepared the solution according to the recipe that you did but I didn't use filtered air system. -when I solved EDTA in water added FeSO_4. 7 H_2 O after that and added this mixture to the boiling solution. I did it in this way and then cover it (with aluminum foil) for avoidance of light, after 2 weeks now it's Purple. I had some rust too and it is normal as... more »
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problem preparing Hutners trace elements...
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Hi everybody,
I've been trying to prepare the Hutner's trace element solution and I've been having some problems, therefore I'm asking for your help.
I prepared the solution according to the recipe provided at the chlamy.org site which is the following one:
For 1 liter final mix, dissolve each compound in the volume of water... more »
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Freeze drying duration time?
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Hi Chlamy-digest
I hope your well.
Like many i use freeze drying as a way to obtain a dry cell weight for my
algal samples prior to further analysis. Recently people have question me
about how long the sample needs to be dried for. According to the
literature it suggest anything from 2 hours to 48 hours.... more »
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sulphur deprivation technique without argon purge
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dear colleagues
please share with me yours effective sulphur deprivation techniques
(Argon free methods are desirable).
In our lab we deprivate a culture accordingly to Melis and live it in
Erlenmeier flasks for 2 days (for exhaustion of all sulphur
leavings) . Then we remove it in hermetic glassware for hydrogen... more »
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