Description:
Research in Caenorhabditis elegans & related nematodes (Moderated)
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To Dear professor Leon Avery,
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Dear professor Leon Avery,
Thanks for your time for reading the letter. I really appreciate it. ^_^
I have read your paper ˇ°Dietary choice behavior in Caenorhabditis elegansˇ± published on J Exp Biol. Your discovery is a breakthrough in C.elegans dietary choice behavior research and has inspired us a lot.... more »
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Single worm reverse-transcriptase (RT) PCR
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Dear colleagues, I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-30}) can be checked by RT-PCR. This requires reverse transcriptase reaction on single worms. Single worm PCR lysis buffer doesn't seem amenable to reverse transcriptase reaction {?}... more »
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Microinjection frustration (Jane Whittingham)
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...Jane, I forwarded your message to our local microinjection expert (Jacob Varkey) and he suggested a different buffer than M9: "Even though it is not necessary, in some cases, I have asked students to use a recovery buffer. There are different formulations of it. One is given below. 3mM HEPES (pH7.2), 3 mM CaCl2, 66mM NaCl, 2.4mM KCl, 4% glucose, 0.1% salmon sperm DNA.... more »
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Microinjection frustration
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I am desparately in need of some help with c. elegans microinjection. I am new to the worm field and have been learning to microinject for about 3 months now and have had absolutely no success whatsoever. I have tried asking other people in the lab to help out and have tried troubleshooting myself from sources on the net or from books on microinjection but nothing has helped. I seem to be doing ok injecting the worms in the right place from what I can tell, my problem is the recovery of the worms. I use M9 buffer for recovery and they initially seem to recover really well, thrashing about in the M9, but once the M9 is absorbed into the plate and the worm adheres to the bacterial lawn, the head is still moving around a lot, but the back end of the worm seems almost paralysed and after a day or so the worm dies. So far the main advice I have had seems to be that there are two main reasons for the worm not recovering: the cuticle is irreparably damaged... more »
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Native protein extraction
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Hi Dr. Aurelio, Greetings. I have just seen your request for assistance in native protein extraction. Am in that dilemma too, so just wondering if you got a solution yet. I want to do enzyme assay in locusts. Thanks and kind regards, Peris
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pmyo2::mcherry line?
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Does anyone have an integrated strain expressing mCherry behind the myo-2 promoter? Our group is interested in using this strain to investigate the role of toxicant exposure in a motion tracking assay. Daniel Snyder Phone: (919) 541-2533 Research Assistant Fax: (919) 541-1460... more »
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Real Time PCR
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Dear colleagues, Is there a consensus on best/good housekeeping gene in C. elegans to use to normalise against in Real Time PCR? Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but find large differences in RNAs from two worm populations (in all replicates). Best regards, G. Joshua.... more »
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Beyond Recognizing Odors, Single Neuron Controls Reactions In Worm
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Babies will smile when they catch the scent of vanilla, but a whiff of rotting meat will send them into fits. From people to mice and flies to worms, animals of all kinds are born with likes and dislikes thanks to the evolutionary wisdom collected in their genes. But new research shows that some preferences are still surprisingly flexible at even... more »
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Advice regarding long-term liquid culture of C. elegans transgenics
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Dear Worm breeders, We have generated a large number of transgenic worms in an unc-119 background using microparticle bombardment. We need large numbers of these transgenics and have been growing them in liquid culture to reach the density required. A few months ago, these strains were growing really well with a quick generation time. More recently, the... more »
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