Help! OP50 Problems!!

[Jennifer Aiello]

Hi

I just read your question about the OP50. I must admit, unfortunately I
don't have an answer. However, I'm one of the college interns working
over at the Dolan DNA Learning Center of Cold Spring Harbor Labs and
I've been involved in our C.elegan project. I've noticed those little
colonies also. No matter how many times I take that one perfect colony
and streak it- I get those little colonies after a week or so.. I was
wondering if you had any suggestions from your post. I was thinking
about searching for other labs that had OP50 and perhaps ordering more.
If you've already tested other OP50's, then maybe that won't work. I
was actually searching for a lab that had OP50 when I stumbled upon
your post.

Thank you for your help

Jenn Aiello